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Mechanisms of bitter taste reception

Research Project

Project/Area Number 11480247
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 神経・脳内生理学
Research InstitutionKeio University

Principal Investigator

KANEKO Akimichi  Keio University, School of Medicine, Professor, 医学部, 教授 (00051491)

Co-Investigator(Kenkyū-buntansha) HIRASAWA Hajime  Keio University, School of Medicine, Research Associate, 医学部, 助手 (00338021)
MIYOSHI Shun-ichiro  Keio University, School of Medicine, Research Associate, 医学部, 助手 (10296577)
KANEDA Makoto  Keio University, School of Medicine, Associate Professor, 医学部, 助教授 (30214480)
AOKI Kaori  Keio University, School of Medicine, Research Associate, 医学部, 助手 (00276213)
TSUNENARI Takashi  Keio University, School of Medicine, Research Associate, 医学部, 助手 (30286439)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2000: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1999: ¥8,000,000 (Direct Cost: ¥8,000,000)
Keywordstoungue / taste receptor cell / bitter sensation / Calcium ion / quinine / patch clamp / noise analysis / power spectrum distribution / 苦み / 単一チャネル記録 / チャネル電導度 / カエル / 苦味 / Na電流 / K電流 / 電気的結合 / 茸状乳頭 / 味壺
Research Abstract

Mechanisms of taste transduction in gustatory receptor cells are not well described. One reason is that most of the past studies have been carried out on isolated taste receptor cells, in which identification of cell types and the cell location are unidentifiable. In the present study we developed a technique to making a slice preparation of the bullfrog fungi form papilla. Voltage-gated ionic current and the response to quinine were studied on the four types of morphologically identified taste cells by whole-cell patch clamp recording with Lucifer yellow-filled pipette. Dye-coupled type la cells (mucous cells) did not show voltage-activated currents. Type Ib cells (wing cells), type II cells (rod cells) and type III cells had voltage-gated sodium (I_<Na>) and potassium currents (I_K) and generated action potentials. The amplitude of Nawas significantly larger in type Ib and II cells than in type III cells. Type Ib and II cells responded to quinine but Type III cells did not.
We also studied the cation current from the frog taste receptor cell activated by quinine. From the variance/mean ratio of the quinine-activated current, the single-channel conductance was estimated to be 12 pS in zero extracellular Ca^<2+>. In the presence of 1.8 mM Ca^<2+>, this conductance decreased to 5 pS. The dependence of the current on quinine concentration had a K_<1/2> of 0.48 mM in the absence of extracellular Ca^<2+>, which increased to 2.8 mM in 1.8 mM external Ca^<2+>. The spectral power density distribution of the quinine-activated current could be described by the sum of two Lorentzian functions, with corner frequencies not substantially different in the absence and presence of 1.8 mM external Ca^<2+>. The above results support the notion that the major component of the response of frog taste receptor cells to quinine comes from an ion channel directly activated by quinine.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (71 results)

All Other

All Publications (71 results)

  • [Publications] Tsunenari T: "Activation by bitter substances of a cationic channel in membrane patches excised from the bullfrog taste receptor cell"J Physiol. 519. 397-404 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko A: "Retinal mechanism of the lateral inhibition and directional selectivity : An old question asked again now"FAOPS Newsletter. 8. 3-5 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aoki K: "Role of Polysialic acid on outgrowth of rat olfactory receptor neurons"Mech Dev. 85. 103-110 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko A: "A novel mechanism of bitter taste transduction"Korean J Physiol Pharmacol. 3. 455-459 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 倉橋 隆: "「香り」の受け方が体内ホルモンの変動によって影響を受ける:アドレナリンを一例にその分子機構と生理学的意味"蛋白質核酵素. 44. 2591-2595 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 竹内裕子: "ウシガエル味蕾を構成する細胞の形態と電気生理学的特徴"日本味と匂学会誌. 6. 523-524 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe S-I: "Tetrodotoxin-sensitive persistent current boosts the depolarization of retinal amacrine cells in goldfish"Neurosci Lett. 278. 97-100 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneda M: "Modulation by Zn2+ of GABA responses in bipolar cells of the mouse retina"Visual Neurosci. 17. 1-9 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe S-I: "GABA-mediatea inhibition between amacrine cells in the goldfish retina"J Neurophysiol. 84. 1826-1834 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 金子章道: "網膜から脳への信号伝達"O plus E. 22. 440-446 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akagi T: "Differential subcellular localization of zinc in the rat retina"J Histochem Cytochem. 49. 87-96 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsunenari T: "Effect of extracellular Ca2+ on the quinine-activated current of bullfrogtaste receptor cells"J Physiol. 530. 235-241 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko A: "Our recent studies on sensory transduction : from vision to taste"Keio J Med. 50. 13-19 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuganezawa H: "A new member of the HCO3-transporter superfamily is an apical anion exchanger of β-intercalated cells in the kidney"J Biol Chem. 276. 8180-8189 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koizumi A: "Persistent Na+ current and Ca2+ current boost grade depolarization of rat retinal amacrine cells in culture"J Neurophysiol. 86. 1006-1016 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Satoh H: "Intracellular chloride concentration is higher in rod bipolar cells than in cone bipolar cells of the mouse retina"Nuerosci Lett. 310. 161-164 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 金田 誠: "網膜神経伝達物質としてみた亜鉛"慶應医学. 78. 171-176 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeuchi H: "Physiology of morphologically identified cells of the bullfrog fungiform papilla"NeuroReport. 12. 2957-2962 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirasawa H: "Analysis of spontaneous EPSCs in retinal horizontal cells in carp"Neurosci Res. 40. 75-86 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirasawa H: "Blocking AMPA receptor desensitization prolongs spontaneous EPSC decay times and depolarizes H1 horizontal cells in carp Retinal slices"Neurosci Res. 40. 217-225 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirasawa H: "A metabotropic glutamate receptor regulates transmitter release from cone presynaptic terminals in carp retinal slices"J Gen Physiol. 119. 55-68 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada Y: "Propagation or action potentials from the soma to the individual dendrites of the cultured rat amacrine cells is independently regulated by local GABA input"J Neurophysiol. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko A: "The Retinal Basis of Vision"Elsevier Science, Amsterdam. 290 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 金子章道: "基礎生化学実験法第3巻"東京化学同人. 229 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsunenari T.: "Activation by bitter substances of a cationic channel in membrane patches excised from the bullfrog taste receptor cell"J. Physiol.. 519. 397-404 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko A.: "The Retinal Basis of Vision Ed Toyoda J"Elsevier Science, Amsterdam. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko A.: "Retinal mechanism of the lateral inhibition and directional selectivity: An old question asked again now"FAOPS Newsletter. 8. 3-5 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aoki K.: "Role of Polysialic acid on outgrowth of rat olfactory receptor neurons"Mech Dev. 85. 103-110 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko A.: "A novel mechanism of bitter taste transduction"Korean J Physiol Pharmacol. 3. 455-459 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kurahashi T.: "Endogenous hormone affects the odorant contrast by modulating signal encoding in olfactory receptor cells: its molecular mechanisms and physiological significance, a case of adrenaline"Tanpakushitu-Kakusan-Koso. 44. 2591-2595 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeuchi H.: "Morphological and Electrophysiological properties of cells consisting the taste bud of the bullfrog"Nihon Aji-to-Nioi-Gakkai Zasshi. 6. 523-524 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe S.-I.: "Tetrodotoxin-sensitive persistent current boosts the dopolarization of retinal amacrine cells in goldfish"Neurosci Lett. 278. 97-100 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneda M: "Modulation by Zn^<2+> of GABA responses in bipolar cells of the mouse retina"Visual Neurosci. 17. 1-9 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe S.-I.: "GABA-mediated inhibition between amacrine cells in theroldfish retina"J Neurophysiol. 84. 1826-1834 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko A.: "Signal transmission from the retina to the brain"O plus E. 22. 440-446 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akagi T.: "Differential subcellular localization of zinc in the rat retina"J Histochem Cytochem. 49 (1). 87-96 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsunenari T.: "Effect of extracellular Ca^<2+> on the quinine-activated current of bullfrog taste receptor cells"J Physiol. 530. 235-241 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko A.: "Our recent studies on sensory transduction: from vision to taste"Keio J Med. 50 (1). 13-19 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuganezawa H.: "A new member of the HCO3-transporter superfamily is anapical anion exchanger of β-intercalated cells in the kidney"J Biol Chem. 276. 8180-8189 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko A.: "Electrophysiological measurement of channel and pump activities"Kiso Seikagaku Jikkenhou Vol. 3, Tokyo Kagaku Doujin. 37-47 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koizumi A.: "Persistent Na^+ current and Ca^<2+> current boost graded depolarization of rat retinal amacrine cells in culture"J Neurophysiol. 86. 1006-1016 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Satoh H.: "Intracellular chloride concentration is higher in rod bipolar cells than in cone bipolar cells of the mouse retina"Neurosci Lett. 310. 161-164 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneda M.: "Zinc as a neurotransmitter in the retina"Keio Igaku. 78. 171-176 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeuchi H.: "Physiology of morphologically identified cells of the bullfrog fungiform papilla"Neuro Report. 12. 2957-2962 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirasawa H.: "Analysis of spontaneous EPSCs in retinal horizontal cells in carp"Neurosci Res. 40. 75-86 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirasawa H.: "Blocking AMPA receptor desensitization prolongs spontaneous EPSC decay times and depolarizes H1 horizontal cells in carp retinal slices"Neurosci Res. 40. 217-225 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirasawa H.: "A metabotropic glutamate receptor regulates transmitter release from cone presynaptic terminals in carp retinal slices"J Gen Physiol. 119. 55-68 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada Y.: "Propagation of action potentials from the soma to theindividual dendrites of the cultured rat amacrine cells is independently regulated by local GABA input"J Neurophysiol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsunenari, T: "Effect of extracellular Ca^<2+> on the quinine-activated current of bullfrog taste receptor cells"Journal of Physiology. 530. 235-241 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuganezawa, H: "A new member of the HCO_3 transporter superfamily is an apical anion exchanger ofβ-intercalated cells in the kidney"Journal of Biological Chemistry. 276. 8180-8189 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Koizumi, A: "Persistent Na^+ current and Ca^<2+> current boost graded depolarization of rat retinal amacrine cells in culture"Journal of Neurophysiology. 86. 1006-1016 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Satoh, H: "Intracellular chloride concentration is higher in rod bipolar cells than in cone bipolar cells of the mouse retina"Neuroscience Letters. 310. 161-164 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeuchi, H: "Physiology of morphologically identified cells of the bullfrog fungiform papilla"NeuroReport. 12. 2957-2962 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamada, Y: "Propagation of action potentials from the soma to the individual dendrites of cultured rat amacrine cells is independently regulated by local GABA input"Journal of Neurophysiology. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] 金子 章道: "基礎生化学実験法第3巻"東京化学同人. 229 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Watanabe S-I: "GABA-mediated inhibition between amacrine cells in the goldfish retina."J Neurophysiol. 84. 1826-1834 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akagi T: "Differential subcellular localization of zinc in the rat retina."J Histochem Cytochem. 49・1. 87-96 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tsunenari T: "Effect of extracellular Ca^<2+> on the quinine-activated current of bullfrog taste receptor cells."J Physiol. 530. 235-24* (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kaneko A: "Our recent studies on sensory transduction : From vision to taste."Keio J Med. 50・1. 13-19 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tsuganezawa H: "A new member of the HCO3-transporter superfamily is an apical anion exchanger of β-intercalated cells in the kidney."J Biol Chem. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 金子章道: "網膜から脳への信号伝達"O plus E. 22. 440-446 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 金子章道: "チャネル、ポンプ機能の電気生理学的測定法基礎生化学実験法第3巻II 機能・動態解析法(日本生化学会編)"東京化学同人(印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Tsunenari T: "Activation by bitter substances of a cationic channel in membrane patches excised from the bullfrog taste receptor cell"J Physiol. 519. 397-404 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kaneko A: "Retinal mechanism of the lateral inhibition and directional selectivity:An old question asked again now"FAOPS Newsletter. 8. 3-5 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Watanabe S-I: "Tetrodotoxin-sensitive persistent current boosts the depolarization of retinal amacrine cells in goldfish"Neurosci Lett. 278. 97-100 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kaneko A: "A novel mechanism of bitter taste transduction"Korean J Physiol Pharmacol. 3. 455-459 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kaneda M: "Modulation by Zn^<2+> of GABA responses in bipolar cells of the mouse retina"Visual Neurosci. 17. 1-9 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 倉橋 隆: "「香り」の受け方が体内ホルモンの変動によって影響を受ける:アドレナリンを一例にその分子機構と生理学的意味"蛋白質核酸酵素. 44. 2591-2595 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Aoki K: "Role of Polysialic acid on outgrowth of rat olfactory receptor neurons"Mech Dev. 85. 103-110 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 竹内裕子: "ウシガエルの味蕾を構成する細胞の形態と電気生理学的特徴"日本味と匂学会誌. 6. 523-524 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kaneko A: "The Retinal Basis of Vision"Elsevier Science,Amsterdam. 290 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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