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EXAMINATION OF OLETF-DERIVED Nidd QTLs BY CONSTRUCTION OF A SERIES OF CONGENIC STRAINS

Research Project

Project/Area Number 11480249
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionThe University of Tokushima

Principal Investigator

MATSUMOTO Kozo  The University of Tokushima, School of Medicine, Associate Professor, 医学部, 助教授 (00002246)

Co-Investigator(Kenkyū-buntansha) KOSE Hiroyuki  The University of Tokushima, School of Medicine, Research Associate, 医学部, 助手 (90314856)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2001: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2000: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1999: ¥6,700,000 (Direct Cost: ¥6,700,000)
Keywordstype 2 diabetes / obesity / OLETF / rat / QTL / congenic strain / Obesity / genome-wide / OLETF rat / loci / Congenic / F344 / Diabetes / Rat / Genetics
Research Abstract

The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is one of the well-characterized animal models for the study of type II diabetes. Our previous QTL mapping identified 14 loci as candidates for the hyperglycemia in the OLETF rat. Here we generated a series of congenic animals by introgressing into normoglycemic F344 background all 14 OLETF-derived diabetic loci. Subsequent OGTT assay revealed that congenic strains for Nidd1, Nidd2, Nidd3 and Nidd10 showed significantly higher levels of blood glucose in comparison with parental host strain, F344. Furthermore, simultaneously made heterozygote animals for Nidd1, 2 and 10 were normoglycemia, indicating that these loci are recessively inherited. The other nine congenic strains were apparently normoglycemic presumably because the effect of a single locus is too small to be detected by the method employed here. These strains are currently raised on high-fat diet to see if dietary manipulation may help manifest genetic contribution of each locus more clearly. Furthermore, reverse congenic strains whereby F344-derived QTLs are introgressed into OLETF background are also under construction. We believe that these congenic strains should provide useful agents for decomposing complex diabetic traits and for subsequent positional cloning.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Yamada T.: "dentification of epistatic interactions involved in non-insulin-dependent diabetes mellitus in the Otsuka Long-Evans Tokushima Fatty rat"Exp.Anim.. 50. 115-123 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minami T.: "Role of Atp7b gene in spontaneous and N-diethylnitrosamine-induced carcinogenesis in a new congenic strain, WKAH.C-Atp7b rats"Jap. J. Cancer Res.. 92. 841-847 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawano H.: "Histological changes in monoaminergic neurons of Long-Evans Cinnamon rats"Brain Res.. 915. 25-31 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Moralejo D.J.: "Gepetic verification of the role of CCK-AR in pancreatic proliferation and blood glucose and insulin regulation using a congenic rat carrying CCK-AR null allele"Res. Com in Mol Patho Pharm.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada,T.: "Identification of epistatic interactions involved in non insulin-dependent diabetes mellitus in the Otsuka Long Evans Tokushima Fatty rat"Exp. Anim.. 50. 115-123 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minami,T.: "Role of Atp7b gene in spontaneous and N-diethylnitosamine-induced carcinogenesis in a new congnic strain, WKAH.C-Atp7b rats"Jap. J. Cancer. 92. 841-847 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawano,H.: "Histological changes in monoaminergic neurons of Long-Evans Cinnamon rats"Brain Res.. 915. 25-31 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Moralejo,D.J.: "Genetic verification of the role of CCK-AR in pancreatic proliferation and blood glucose and insulin regulation using a congenic rat carrying CCK-AR null allele"Res. Com in Mol Patho Pharm. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamad T.: "dentification of epistatic interactions involved in non-insulin-dependent diabetes mellitus in the Otsuka Long-Evans Tokushima Fatty rat"Exp. Anim.. 50. 115-123 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Minami T.: "Role of Atp7b gene in spontaneous and N-diethylnitrosamine-induced carcinogenesis in a new congenic strain, WKAH.C-Atp7b rats"Jap. J. Cancer Res.. 92. 841-847 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kawano H.: "Histological changes in monoaminergic neurons of Long-Evans Cinnamon rats"Brain Res.. 915. 25-31 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Moralejo D.J.: "Genetic verification of the role of CCK-AR in pancreatic proliferation and blood glucose and insulin regulation using a congenic rat carrying CCK-AR null allele"Res. Com in Mol Patho Pharm.. (In press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Ogino et al.,and Matsumoto: "Genetic evidence for obesity loci involved in the regulation of the body fat distribution in obese type 2 diabetes rat, OLETF."GENOMICS. 70. 19 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamada et al.,and Matsumoto: "Identification of epistatic interactions involved in non-insulin-dependent diabetes mellitus in the Otsuka Long-Evans Tokushima"Exp.Animal. (In press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Narita et al.,and Matsumoto: "Dissociation of Epistatic Effects Involved in Fasting and Postprandial Hyperglycemia"Res.Comm.Mol.Pahtol.Pharmacol.. 107. 79 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugiura,et al.,and Matsumoto,K: "Identification of novel non-insulin-dependent diabetes mellitus susceptibility loci in the Otsuka Long-Evans Tokushima Fatty rat by MQM-mapping method"Mammalian Genome. 10. 1126-1131 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wei,k,et al.and Matsumoto,K: "An efficient multiplex PCR suitable for large scale typing in linkage mapping"J.Vet.Med.Sci. 61. 849-851 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wei,S.,et al.and Matsumoto,K: "Mapping and characterization of quantitative trait loci for non-insulin-dependent diabetes mellitus with an improved genetic map in the Otsuka Long-Evans Tokushima Fatty rat"Mammalian Genome. 10. 249-258 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ogino,T.,et al and Matsumoto,K: "Identification of possible quantitative trait loci responsible for hyperglycemia after 70% pancreatectomy using a spontaneously diabetogenic rat"Genetical Res.. 73. 29-36 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamada,T.,et al.Matumoto,K.(分担): "Obesity and NIDDM : Lesson form the OLETF rat"ELSEVIER. 248(13〜26) (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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