| Project/Area Number |
11480259
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kagoshima University |
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Principal Investigator |
AKASHI Mitsuru Kagoshima University, Faculty of Engineering, Professor, 工学部, 教授 (20145460)
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| Co-Investigator(Kenkyū-buntansha) |
SERIZAWA Takeshi Kagoshima University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (30284904)
BABA Masanori Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (70181039)
岸田 晶夫 国立循環器病センター研究所, 生体工学部, 部長 (60224929)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥11,200,000 (Direct Cost: ¥11,200,000)
Fiscal Year 2001: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | polymeric nanospheres / self-assembling / biopolymers / peptides as medicine / carbohydrates / phospholipids / lectin / HIV vaccine / エイズワクチン / 高分子微粒子 / 糖鎖 / タンパク質 / レクチン / ポリスチレン / ポリスチレンナノスフェア / 糖鎖固定化 / グリボトリオース固定化 / ベロ毒素補足 / レクチン固定化 / HIV-1捕捉 |
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| Research Abstract |
Free radical dispersion copolymerization of hydrophobic monomers with hydrophilic macromonomers in a polar solvent without any emulsifier gives monodispersed polymeric nanospheres, and the nanosphere formation mechanism based on self-assembling of multiphase polymers suggests that hydrophilic macromonomer chains cover the surface of the nanospheres to reduce core-corona polymeric nanospheres. Using the macromonomer method, a variety of core-corona polymeric nanospheres can be prepared and their surface can be easily modified by macromonomer chains.
In this study, biopolymers or biomolecules such as (1) peptides as medicine, (2) carbohydrates for the recognition of proteins, (3) phospholipids analog for giving a bioinspired polymer surface, and (4) lectin and HTV-1 for capturing HIV-1 and HIV-1 vaccine, respectively, were immobilized on the corona of polymeric nanospheres. We concluded that the optimum hybridization of self-assembling polymeric nanospheres and biopolymers is a key of highly functional particle preparation and important for design of biomedical materials.
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