| Project/Area Number |
11480262
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kansai University |
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Principal Investigator |
OHBA Kenkichi Kansai University, Faculty of Engineering, Professor, 工学部, 教授 (30029186)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Takashi Kansai University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (50239414)
BANDO Kiyoshi Kansai University, Faculty of Engineering, Professor, 工学部, 教授 (70156545)
URAGAMI Tadashi Kansai University, Faculty of Engineering, Professor, 工学部, 教授 (80067701)
SAKURAI Atsushi Kansai University, Faculty of Engineering, Lecturer, 工学部, 専任講師 (50162334)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2001: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1999: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | Model erythrocyte / Tiny polymeric gel particle / Elasto-plastic deformation / Coefficient of surface extension / Hertz's conact theory / Deformability / Model human blood / Artifical erythrocyte / ミクロンサイズ粒子 / 高分子ゲル粒子 / 粘弾性粒子 / 塑性変形 / 応力緩和 / 粒子のヤング率 / 粒子の変形能 / 柔軟弾塑性粒子 / ずり応力-ずり速度図 / カッソン流体 / 高分子ゲル粒子分散液 / 見かけ粘度 |
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| Research Abstract |
We have developed a tiny particle of natural polymeric gel as a model of human red blood cell (RBC), and have made a high concentration suspension of these particles in water and physiological salt solution as a realistic model of human blood. The model RBC (MRBC) was made of alginic acid calcium gel, and was of a spherical shape and very soft and elastic. Its average diameter was about 10 μm, which was very similar to human RBC, although its shape was not so-called biconcave. It was shown that the relationships between shear rate dγ/dt and shear stress τ for the human blood with 45% hematocrit (volumetric concentration) of the RBC at the temperature of 37℃ and for the model blood with 45% hematocrit of the MRBC at the temperature of 15℃ agreed with each other very well on the Casson's diagram, i.e. the square root of dγ/dt versus √τ. The coefficients of viscosity calculated from the above diagram in the case of several different concentrations and temperatures showed clear shear thinning, and agreed very well with that of the human blood. The motion of each MRBC in the various range of shear rate was observed using a rheoscope system consisted of an inverted microscope, a cone-plate viscometer, and a high-speed video camera. As a result, in the region of low shear rate less than about 100/sec, MRBC was seen to form aggregates, which was a very similar phenomenon as real RBC forms a rouleaux when shear rate is lower than about 100/sec. The elasticity of the MRBC was also visualized and measured using a micromanipulator. The relationship between the deformation of the spherical MRBC and the force applied on it obeyed the Hertz's contact theory, and the total Young's modulus of the MRBC was 0.075MPa for ν= 0.5 and 0.lOMPa for ν= O, where ν is the Poisson's ratio. These numerical values were almost same as those of gelatin.
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