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Development of the culture methods of marine ostracodes for the environmental indicator species and paleobiological research

Research Project

Project/Area Number 11554020
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Stratigraphy/Paleontology
Research InstitutionShizuoka University

Principal Investigator

IKEYA Noriyuki  Shizuoka University, Faculty of Science, Professor, 理学部, 教授 (50022223)

Co-Investigator(Kenkyū-buntansha) HORIUCHI Jun  Seikan Inspection Center (K.K.), Ecology Section, Chief Researcher, 生態研究部門, 研究員
TSUKAGOSHI Akira  Shizuoka University, Faculty of Science, Associate Professor, 理学部, 助教授 (90212050)
SUZUKI Yoshimi  Shizuoka University, Faculty of Science, Professor, 理学部, 教授 (30252159)
ISHIKAWA Yoshiro  Institute for Environmental Sciences, Department of Environmental Simulation, Chief Researcher, 環境シミュレーション部, 研究員
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥10,900,000 (Direct Cost: ¥10,900,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2000: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1999: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordslife history / coral reef / ostracodes / laboratory culturing / organic carbon cycle / trace element / environmental indicator species / littoral environment / Mother Culture / Mono Culture / 介形虫 / 環境条件 / Mother Cultuer / Mono Cultuer
Research Abstract

Two kinds of culturing system were planned in order to obtain a continuous supply of experimental organisms for paleobiological research. Firstly, a mother culture system having a near natural marine environment was tried. This culture, system had multiple species living together. The second system involved culturing of single species isolated in small petri dishes within the incubator.
In the mother culture system, a rocky shore environment was experimented, and microhabitat of ostracodes were observed. Contrary to the general belief that certain ostracodes species lives in the calcareaous algae, the present results show that different species have different habitats. Some species live on the leaves, some others live surrounding the root of the weeds whereas other species live in the sediments. However, there are certain problems related with this culture system. As the number of individuals in a species reduces during the experiment, frequent addition of individuals from the natural habitat is essential. If it is possible to maintain a healthy growing sea weeds in the culture system, the number of individuals can be kept constant. The possible other factors include ultraviolet irradation and fast circulation of sea water.
In the monoculture system, the Sr/Ca and Mg/Ca ratios were analyzed for the species of Xestoleberis hanaii. The results show that Sr/Ca ratio is independent of sea water temperature, where as the Mg/Ca ratio is dependent on sea water temperature. Especially, the ontogenetical observation of the chemical parameters prove that Mg is concentrated in organic layer within the carapace. This indicates that Mg has an important role in calcification process of the carapace. In this system, the problem of bacteria can be reduced by replacing not only the sea water but also the petri dish itself. Further, the quantity of food should be very less for this system and hence easy to culture.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] 鈴木 款: "海洋による二酸化炭素の吸収:プランクトンの役割"Plankton. Soc. Japan. 48. 22-23 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] L.Wang: "Isolation of nano-coololdal organic matter from coastal Boral bay seawater using porous galss filter as cross-flow filtration membrane"Geoscience Reports of Shizuoka University. 28. 76-82 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 鈴木 款: "生物によるCO_2固定"日本エネルギー学会誌. 80. 818-827 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 大西由香: "海水中における有機物分解過程:植物プランクトンを用いた分解実験"日本海水学会誌. v.56,no.1. 17-25 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fuji, M.: "Decomposition processs of phytoplankton in sea water 1. Kinetic analysis on the effect of organic matter concentration"Journal of Oceanography. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Dennis A.: "Dissolved Organic Carbon Export with North Pacific Intermediate water Formation"Global biogeochemical Cycle. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中尾有利子: "古生物の科学3 古生物の生活史(池谷仙之・棚部一成編)"朝倉書店. 282(239-245) (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, Y.: "Oceanic sink for atmospheric CO^2 : Role of phytoplankton"Bull. Plankton Soc. Japan. 48. 22-23 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] L. Wang: "Isolation of nano-cooloidal organic matter from coastal Boral bay seawater using porous galss filter as cross-flow filtration membrane"Geoscience Reports of Shizuoka University. 28. 76-82 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, Y.: "CO^2 sequestration by biological system of land and ocean"Journal of the Japan Institute of Energy. 80. 818-827 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Onishi, Y.: "Decomposition process of organic matter in sea water using phytoplankton : Effect of the difference in initial concentration of organic matter"Bull. Soc. Sea water Sci. Jpn.. v.56, no. 1. 17-25 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fuji, M.: "Decomposition processs of phytoplankton in sea water 1. Kinetic analysis on the effect of organic matter concentration"Journal of Oceanography. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Dennis A.: "Dissolved Organic Carbon Export with North Pacific Intermediate water Formation"Global biogeochemical. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 鈴木 款, 大西由香, 藤井 実: "海洋による二酸化炭素の吸収:プランクトンの役割"Plankton.Soc.Japan. 48. 22-23 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] L.Wang, Y.Suzuki, H.Ito: "Isolation of nano-cooloidal organic matter from coastal Boral bay seawater using porous galss filter as cross-flow filtration membrane"Geoscience Reports of Shizuoka University. 28. 76-82 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 鈴木 款: "生物によるCO_2固定"日本エネルギー学会誌. 80. 818-827 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 大西由香, 藤井 実, 村重慎一郎, 湯沢 篤, 宮坂 均, 鈴木 款: "海水中における有機物分解過程:植物プランクトンを用いた分解実験"日本海水学会誌. v.56,no.1. 17-25 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fuji, M., S.Murashige, Y.Onishi, Y.Suzuki: "Decomposition process of phytoplankton in sea water 1. Kinetic analysis on the effect of organic matter concentration"Journal of Oceanography. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Dennis A.Hansell, Yoshimi Suzuki, Steven Emerson, Craig Carlson: "Dissolved Organic Carbon Export with North Pacific Intermediate water Formation"Global biogeochemical Cycle. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中尾 有利子, 塚越 哲: "古生物の科学3 古生物の生活史(池谷仙之・棚部一成編)"朝倉書店. 282(239-245) (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ikeya,N.and Kato,M.: "The life history and culturing of Xestolberis hanaii (Crustacea,Ostracoda)"Hydrobiologia. v.419. 149-159 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tsukagoshi,A.and Parker,A.R.: "Trunk segimentation of some podocopine line ages in Ostracoda"Hydrobiologia. v.419. 15-30 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Abe,K.and Horiuchi,J.: "Reproductive strategy of an isopod Onisocryptus ovalis,parasitizing a bioluminescent myodocope ostracod Vargula hilgendorfii"Hydrobiologia. v.419. 191-197 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 黒沢勝彦,鈴木款: "サンゴ礁における窒素・リン循環の複合モデル"静岡大学地球科学研究報告. 26. 61-69 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 須勝雅夫,居波永治,鈴木款,皆川昌幸: "海水中溶存二酸化炭素の連続計測のための光ファイバーセンサーの開発"日本海水学会誌. 54. 21-29 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 鈴木款,大西由香,藤井実: "海洋による二酸化炭素の吸収:プランクトンの役割"Plankton.Soc.Japan,. 48. 22-23 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 中尾有利子,塚越哲: "古生物の科学3 古生物の生活史(池谷仙之,棚部一成 編)"朝倉書店. 282(239-245) (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ikeya,N.and Kato,M.: "The life history and culturing of Xestoleberis hanaii (Crustacea,…)"Hydrobiologia. (in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsukagoshi,A.and Parker,A.R.: "Trunk segimentation of some podocopine line ages in Ostracoda"Hydrobiologia. in press (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Abe,K.and Horiuchi,J.: "Reproductive strategy of an isopod Onisocryptus ovalis,....."Hydrobiologia. (in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 黒沢勝彦、鈴木 款: "サンゴ礁における窒素、リン循環の複合モデル"静岡大学地球科学研究報告. 26. 61-69 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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