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Single-Microparticle Based Analyses under Solution Flow Conditions

Research Project

Project/Area Number 11554032
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 分離・精製・検出法
Research InstitutionThe University of Tokyo (2000-2001)
Hokkaido University (1999)

Principal Investigator

KIM Haeng-boo  The University of Tokyo, School of Engineering, Assc. Prof., 工学部・附属総合試験所, 助教授 (40186367)

Co-Investigator(Kenkyū-buntansha) HISAMOTO Hideaki  The University of Tokyo, School of Engineering, Lecturer, 大学院・工学系研究科, 講師 (00286642)
KITAMORI Takehiko  The University of Tokyo, School of Engineering, Prof., 大学院・工学系研究科, 教授 (60214821)
セルゲイ クラユシュキン  (株)東京インスツルメンツ, レーザー計測開発課, 係長(研究職)
SATOH Kiichi  The University of Tokyo, School of Engineering, Res.Assc., 大学院・工学系研究科, 助手 (50321906)
KRAYUSHKIN Sergey V.  Tokyo Instruments, Inc., Assistant Manager
石坂 昌司  北海道大学, 大学院・理学研究科, 助手 (80311520)
八尾 浩史  北海道大学, 大学院・理学研究科, 助手 (20261282)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2001: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2000: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥6,300,000 (Direct Cost: ¥6,300,000)
Keywordsmicrospectroscopy / laser-trapping / flow cell / extraction / solid phase concentration / microparticle / dye staining of living cell / イオン交換樹脂
Research Abstract

Microspectroscopy and microelectrochemical systems combined with a fluid manifold were developed to manipulate : and analyze single microparticles such as polymer beads, oil droplets, living cells, and so forth. By using a flow cell set on a microscope stage, an aqueous solution containing microparticles is introduced to the cell. With a single particle being fixed by laser-trapping for polymer beads, on the top end of a microcapillary for oil-droplets, or on the wall by adhesion for living-cells, other non-fixed particles are pumped out completely by flow of sufficient amount of water to the cell. These procedures can hold exclusively the single particle in the cell. By using an appropriate fluid manifold, furthermore, an arbitrary reagent can be introduced to the cell, so that a temporal profile of chemical/physical responses of the particle would be monitored by microspectroscopic and/or microelectrochemical method(s) without interference by other particles. Three different chemical/biochemical processes were studied under solution-flow conditions :
1) Ion-exchange dynamics of a single ion-exchange-resin bead by laser trapping-absorption microspectroscopy.
2) Liquid/liquid extraction dynamics of a single oil-droplet by microinjection - microspectroscopy.
3) Dye-staining dynamics of a single living-cell (Saccharomyces cerevisiae)
The ion-exchange, the extraction, or the staining rate and efficiency were discussed in terms of the solution flow rate and the particle diameter.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Osamu Kogi: "Auto-Oscillated Vibration of Single Micrometer-Sized Oil Droplets in Aqueous Surfactant Solution"Langmuir. 24. 7456-7458 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yuki Tanaka: "Acceleration of an Enzymatic Reaction in a Microchip"Anal. Sci.. 17. 809-810 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osamu Kogi: "Microinjection-Microspectroscopy of Single Oil Droplets in Water : An Application to Liquid/Liquid Extraction under Solution-Flow Conditions"Anal. Chim. Acta. 418. 129-135 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Haeng-Boo Kim: "A Spatially-Resolved Fluorescence Spectroscopic Study on Liquid/Liquid Extraction Processes in Polymer Microchannels"Anal. Sci.. 16. 871-876 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Noboru Kitamura: "Space-and Time-Resolved Spectroscopic Analyses in Micrometer Dimension"Trends in Anal. Chem.. 18. 675-684 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Haeng-Boo Kim: "Single-Microparticle Measurements : Laser Trapping-Absorption Microspectroscopy under Solution-Flow Conditions"Anal. Chem.. 71. 4338-4343 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] O. Kogi: "Auto-Oscillated Vibration of Single Micrometer-Sized Oil Droplets in Aqueous Surfactant Solution"Langmuir. 24(17). 7456-7458 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Tanaka: "Acceleration of an Enzymatic Reaction in a Microchip"Anal. Sci.. 17(7). 809-810 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Habuchi: "Water Structure in Ion -Exchange Resin Particles : Solvation Dynamics of Nile Blue A"Anal. Chem.. 73(2). 366-372 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Ueno: "Fabrication and Characteristic Responses of Integrated Microelectrodes in Polymer hannel Chip"Chem. Lett.. (8). 858-859 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] O. Kogi: "Microinjection-Microspectroscopy of Single Oil Droplets in Water : An Application to Liquid/Liquid Extraction under Solution-Flow Conditions"Anal. Chim. Acta. 418. 129-135 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.-B. Kim: "A Spatially -Resolved fluorescence Spectroscopic Study on Liquid/Liquid Extraction Processes in Polymer Microchannels"Anal. Sci.. 16(8). 871-876 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Habuchi: "Chemical Size -Efefcts on Fluorescence Lifetime of Rhodamine 6G in Ethylene Glycol/Water Microdroplets Dispersed in Polydimethylsiloxane Matrix."J. Photochem. Photobiol. A : Chem.. 133(3). 189-196 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N. Kitamura: "Space- and Time -Resolved Spectroscopic Analysis in Micrometer Dimension"Trends in Anal. Chem.. 18(11). 675-684 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Ishizaka: "Excitation Energy Transfer from Sulforhodamine 101 to Acid Blue 1 at a Liquid/Liquid Interface : Structural Dimension of the Interface"Anal. Chem.. 71(16). 3382-3389 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.-B. Kim: "Dynamic Fluorescence Spectroscopic Study on the Microstrucrtures in Ion -Exchange Resion Particles."Anal. Chem.. 71(4). 842-848 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.-B. Kim: "Single-Microparticle Measurements : Laser Trapping-Absorption Microspectroscopy under Solution-Flow Conditions"Anal. Chem.. 71(19). 4338-4343 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osamu Kogi: "Auto-Oscillated Vibration of a Micrometer-Sized Oil-Droplet in Aqueous Surfactant Solution"Langmuir. 24(17). 7456-7458 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Satoshi Habuchi: "Water Structures in Ion-Exchange Resin Particles : Solvation Dynamics of Nile Blue A"Anal. Chem.. 73(2). 366-372 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 金 幸夫: "化学反応場としてのマイクロ化学チップ-マイクロ化学プラントへの技術と展望-"機械振興. 34(4). 42-49 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 金 幸夫: "集積化ミクロ化学システム"マテリアルインテグレーション. 15(2). 3-8 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Haeng-Boo.Kim: "Microinjection-Microspectroscopy of Single Oil Droplets in Water : An Application to Liquid/Liquid Extraction under Solution-Flow Conditions."Anal.Chim.Acta.. 418・2. 129-135 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kousei Ueno: "Fabrication and Characteristic Responses of Integrated Microelectrodes in Polymer Channel Chip."Chem.Lett.. ・8. 858-859 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Satoshi Habuchi: "Chemical Size Effects on Fluorescence Lifetime of Rhodamine 6G in Ethylene Glycol/Water Microdroplets Dispersed in Plydimethylsiloxane Matrix"J.Photochem.Photobiol.A : Chem.. 133・3. 189-196 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kiichi Sato: "Integration of an Immunosorbent Assay System : Analysis of Secretory Human Immunoglobulin A on Polystyrene Beads in a Microchip"Anal.Chem.. 72・6. 1144-1147 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.-B. Kim: "Dynamic Fluorescence Spectroscopic Study on the Microstructures in Ion-Exchange Resin Particles"Analytical Chemistry. 71(4). 842-848 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] N. Kitamura: "Space- and Time-Resolved Spectroscopic Analyses in Micrometer Dimension"Trends in Analytical Chemistry. 18(11). 675-684 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.-B. Kim: "Single-Microparticle Measurements : Laser Traping-Absorption Microspectroscopy under Solution-Flow Conditions"Analytical Chemistry. 71(19). 4338-4343 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S. Habuchi: "Chemical Size-Effects on Fluorescence Lifetime of Rhodamine 6G in Ethylene Glycopl/Water Microdroplets Dispersed in Polydimethylsiloxane Matrix"Journal of Photochemistry and Photobiology A : Chemistry. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] N. Kitamura: "Spectroscopic Analysis of Inhomogeneous Structures in Single Microparticles. in "Mesoscopic Chemistry""Blackwell Science Ltd., IUPAC Monograph on the 21st Century Chemistry. 14 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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