| Project/Area Number |
11555211
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
反応・分離工学
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| Research Institution | KOCHI NATIONAL COLLEGE OF TECHNOLOGY |
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Principal Investigator |
NAGAYAMA Kazuhito KOCHI NATIONAL COLLEGE OF TECHNOLOGY, DEPARTMENT OF MATERIALS SCIENCE AND ENGINEERING, RESEARCH ASSOCIATE, 物質工学科, 助手 (00270364)
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| Co-Investigator(Kenkyū-buntansha) |
NAOE Kazumitsu NARANATIONAL COLLEGE OF TECHNOLOGY DEPARTMENT OF CHEMICAL ENGINEERING, LECTURER, 物質化学工学科, 講師 (00259912)
IMAI Masanao NIHON UNIVERSITY, DEPARTMENT OF FOOD SCIENCE AND TECHNOLOGY COLLEGE OF BIORESOURCE SCIENCES, ASSOCIATE PROFESSOR, 生物資源科学部・食品科学工学科, 助教授 (80193655)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1999: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Microemulsion / Reverse micelle / Organogel / Lecithin / Sugar ester / Polyglycerol ester / Esterification / Lipase |
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| Research Abstract |
The reactivity and productivity of fatty acid esterification catalyzed by lipases were investigated in the biocompatible nano-scale assemblages, i.e. microemulsion (reverse micelle) and microemulsion-based organogel systems.
1. Microemulsion system
In the microemulsion systems formed by sugar ester and polyglycerol ester as biocompatible amphiphiles, the maximal reaction rate of lipases (Mucor miehei and Rhizopus delemar) was determined as a function of the amphiphile and water concentrations. The catalytic activity was evaluated in terms of the kinetic parameters. The lipases were recovered by contacting the microemulsion phase with a stripping aqueous phase. The findings show that these systems are an effective reaction media following the recovery of lipase.
2. Microemulsion-based organogel system
In the lecithin microemulsion-based organogels formed by the addition of gelatin, the maximal reaction rate of immobilized lipases (Candida rugosa, Mucor javanicus, Rhizopus arrhizus and Rhizopus delemar) was determined as a function of the water, gelatin and lecithin concentrations. The reactivity was estimated as a function of the radius of gel particle by calculating the effectiveness factor. In the batch and continuous reactions, the immobilized lipase activity was well preserved over a long period, and a high level conversion was attained. The findings show that this system is an effective immobilized media for fatty acid esterification catalyzed by lipase.
3. Microstructure and properties of microemulsions
The microstructure and properties of the microemulsions were investigated by measuring electric conductivity. The interaction between the microemulsions markedly changed by the operational temperature. The interaction differed with the solubilization of electrolytes and enzymes into the microemulsion water phase and the species of organic solvent.
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