| Project/Area Number |
11555224
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
工業分析化学
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| Research Institution | IBARAKI UNIVERSITY |
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Principal Investigator |
IGARASHI Shukuro IBARAKI Univ.Fac.of Engineering Assistant Professor, 工学部, 助教授 (70150258)
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| Co-Investigator(Kenkyū-buntansha) |
OHTO Tokio Fuji Electric Corporate Research and Development, Ltd.Group Manager of Enviro.Tech.Lab., 化学・バイオ計測器グループ, 研究マネージャー(研究職)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2000: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1999: ¥8,100,000 (Direct Cost: ¥8,100,000)
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| Keywords | Down sizing / Concentration method / Homogeneous liquid-liquid extraction method / Fluorosurfactant / Microdroplet on filter-paper / Endocrine disrupting chemicals / Spectrofluorimetry / X-ray fluorescence spectrometry / 蛍光X線分析法 / アミノ酸 / 点滴ろ紙法 / 蛍光光度法 / 金属-ポルフィリン錯体 / HPLC / MS法 |
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| Research Abstract |
The aim of this study is that fuses together with the minuteness of sample solution and the analytical apparatus which directs toward "Down Sizing". The homogeneous liquid-liquid extraction was studied in detail as a high-speed and high-powered concentration method, in order to create the total down sizing analytical system.
The produced fruits during the term of two years are as follows :
1.Development of some chromatographies following homogeneous liquid-liquid extraction.
(1)HPLC analytical system : New homogeneous liquid-liquid extraction was developed using pH dependent phase separation phenomenon in water/pyridine/ethyl chloroacetate ternary component solvent system. In this method, the simultaneous determination of metal ions was performed by spectrophotometric detection-HPLC with water-soluble porphyrin.
(2)GC/MS analytical system : Above homogeneous liquid-liquid extraction was also applied as pretreatment of GC/MS.As results, twelve kinds of chlorophenol compounds which are doubted as endocrine disrupting chemicals, were simultaneously and rapidly determined.
2.Development of some spectrometries following a microdroplet on filter-paper and a homo-geneous liquid-liquid extraction with perfluorosurfactant.
(1)Flurescence spectrometry : The pH dependent phase separation using perfluorosurfactant was studied as preconcentration method. Among 22 kinds of amino acid, tryptophan was selectively determined by the fluorometry.
(2)X-ray fluorescence spectrometry : Fifteen kinds of element containing high valence metals were simultaneously determined. Moreover, new concept which uses together three kinds of chelating reagents in the same process, was proposed.
In conclusion, the satisfactory results were obtained in this project according to the original plan. In the near future, this useful method will be applied to wide range of practical sample.
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