| Project/Area Number |
11555227
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
工業分析化学
|
|---|
| Research Institution | Hachinohe National College of Technology (2000-2001)
Ariake National College of Technology (1999) |
|---|
Principal Investigator |
ASANO Yasukazu Department of chemical & Biological Engineering, Hachinohe National College of Technology, Professor, 物質工学科, 教授 (80311108)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAHEI Hisao Material Technology Dept. Device Development dept, NTT advanced Technology Corp., General Manager, 主幹部長
IMATO Toshihiko Graduate School of Engineering, Kyushu University, Professor, 大学院・工学研究院, 教授 (50117066)
MASADOME Takashi Department of chemical & Biological Engineering, Hachinohe National College of Technology, Associate Professor, 物質工学科, 助教授 (30190341)
|
|---|
| Project Period (FY) |
1999 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2000: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥6,000,000 (Direct Cost: ¥6,000,000)
|
|---|
| Keywords | endocrine disruptors / environmental pollution / surface-plasmon resonance phenomenon (SPR)-based chemical sensors / field analysis / PVC film alternative to matching oil |
|---|
| Research Abstract |
A simple analytical instrument based on surface plasmon resonance(SPR) for the the rapid screening toxic organic substance in environment samples has been needed for field use.
First, since conventional matching oil, which is used as the light interface between a prism and an Au coated cover glass, is unsuitable for field use, we developed a new light interface membrane based on a plasticized PVC. The membrane performance was best in the ratio of 0.5 di-2-ethylhexyl phthalate to 0.5 g tritol phosphate in 0.2g PVC at 80ーC
Second, in order to enhance the sensitivity of SPR signal, the efficient immobilization of anti-IgG to Au surface was investigated. The large SPR signal was obtained in the immuno-reaction between IgG and anti-IgG using 10-Carboxyl 1-decanethiol as the treatment agent in the test with some kinds of thiols.
Consequently, the good calibration curve was obtained in the range 0.6〜3.O× 10-3 mM 2, 4-di-chlorophenol, which was similar to dioxin.
From these experimental results, it became clear that SPR chemical sensor for the toxic low molecule organic substance like environment hormones could be constructed by development of the much more efficient immobilization-technique of antibody on Au surface.
|
