| Project/Area Number |
11555228
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
工業物理化学
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
OYAMA Noboru Tokyo University of Agriculture and Technology, Faculty of Technology, Professor, 工学部, 教授 (40134845)
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| Co-Investigator(Kenkyū-buntansha) |
NAMBO Yuichiro Eisai Corporation, Development of Clinical Diagnostics, Manager, 診断薬部, 課長(研究職)
HABA Masanori Meidensha Corporation, Advanced Technology Center, Research Leader, 総合研究所, 研究部長
HATOZAKI Osamu Tokyo University of Agriculture and Technology, Faculty of Technology, Research Associate, 工学部, 助手 (40313291)
難波 祐三郎 エーザイ株式会社, 診断薬部, 課長(研究職)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,700,000 (Direct Cost: ¥13,700,000)
Fiscal Year 2000: ¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1999: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | quartz crystal microbalance / electrochemiluminescence / multichannel sensors / magnetic microbeads / highly sensitive biosensors / immunoassay / clinical medicine / 臨床医学 / マルチアレー |
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| Research Abstract |
The subject of this research supported by Grant-in-Aid for Scientific Research (B)(2) is to develop highly sensitive multichannel quartz crystal microbalance sensors in combination with electrochemiluminescence.
Principles and methods for scanning electrode quartz crystal analysis were established, in which a microelectrode was scanned over a quartz crystal, the opposite side of which was coated with a normal electrode. It was found that qualitative mapping of the mass distribution as well as distributions of viscoelastic changes in surface layers on the quartz substrate was possible in the lateral resolution of 1mm for a 9MHz quartz crystal.
Further development based on the scanning electrode quartz crystal microbalance was carried out to fabricate a multichannel quartz crystal microbalance (MQCM), by arranging arrays of quartz crystal resonators on a single quartz wafer. Several types of MQCM with 4-channels were made and characterized. As a result, it is confirmed that there were no interference among resonators on an MQCM and thus each resonator is possible to function as an independent detection device. Simultaneous measurements of multi-component were successfully conducted using MQCM.
Development of highly emissive electrochemiluminescence (ECL) systems was another subject of this research. Effects of parameters such as TPA concentration, pH, etc., were examined for an ECL system based on Ru(bpy)3^<2+> and tripropylamine (TPA). It was found that addition of NaN3 enhanced the intensity of the luminescence from the complex. Furthermore, surfactants also affected the ECL responses of the Ru(bpy)3^<2+>-TPA system. ECL responses of a Ru complex derivative immobilized on a magnetic microbead surface was also studied. The intensity of ECL from magnetic microbeads was strongly dependent on aggregation of the microbeads.
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