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Establishment of standard experimental strains through pure-line selection in major weeds in Japan

Research Project

Project/Area Number 11556010
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 植物保護
Research InstitutionOsaka Prefecture University

Principal Investigator

YAMAGUCHI Hirofumi  Graduate School of Agriculture and Biological Sciences, Professor, 農学生命科学研究科, 教授 (20112542)

Co-Investigator(Kenkyū-buntansha) UMEMOTO Shinya  Graduate School of Agriculture, Kyoto University, Assistant, 農学研究科, 助手 (60213500)
OKI Yoko  Faculty of Environmental Science and Technology, Okayama University, Professor, 環境理工学部, 教授 (30127550)
SAGO Ryuichi  School of Agliculture, Ibaraki University, Associate Professor, 農学部, 助教授 (30261746)
TAKESHITA Takafumi  Research Institute of Plant Regulator Association, Director, 所長
ISHIKAWA Shizuko  National Agriculture Research Center for Hokkaido Region, Chief Researcher, 主任研究官
Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥11,800,000 (Direct Cost: ¥11,800,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥6,500,000 (Direct Cost: ¥6,500,000)
Keywordsweeds / standard experimental strains / selection breeding / herbicide tolerance / microsatellites
Research Abstract

For establishing the scientific foundation to principles on breeding the standard experimental strains in Japanese major weeds, 1267 accessions of gramineus species from different local area were collected with voucher herbarium specimens. Inbreeding by panicle-bagging and bulk maintaining of seeds were conducted with DNA polymorphism evaluation. Bred accessions and previously bred strains were performed in physio-ecological evaluation. Programs were scheduled all the processes from seed collection through breeding and selection of representative strains to seed production of established strain. Practices of all the process were thoroughly evaluated. In Echinochloa weed, as a model taxon, molecular genetic skills were applied for species identification and tagging of experimental strains. Molecular discrimination practice was established by the PCR-RFLP in Echinochloa crus-gslli and E. otyzicola. Eight mJcrosatellites were established using one accession of Echinochba. Since four of th … More em were polymorphic in Echinochloa weeds, their applicability was tested by evaluation of inbred strains. As a result 44 accessions of third inbred generation were selected as mother strains of standard experimental strains of which identities and uniqueness were proved by microsatellite variations. Flowering habit, precocity, seed production ability and genetic variation for chemical tolerance were evaluated in established strains in several species of arable (Rumex obtsusifolius, Rorripa islandica), paddy (Echinochloa crus-galii and E. otyzicola), subtropical (Digitaria, Echinochloa, Poa, Paspalum) and aquatic (Eichhornia crassipes) weeds. In general ecophysiological teaits were much variable among strains, but RAPD variation was narrow and AFLP diversity showed geographical gradient. In particular Japanese populations of Echinochloa showed no herbicide-tolerance. The selected strains including standard strains will be provided freely for academic scientific research upon request as informed at an international conference. Less

Report

(5 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Umemoto, S.: "Immigration mode of Eclipta into Japan"Scientific Report of College of Agriculture, Osaka Prefecture University. 51. 25-31 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Umemoto, S.: "Is Poa crassinervis Honda endemic to Japan"Proceeding of 17 th APWSS Conference, Beijing. I(B). 610-613 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 大江真道: "ノビエにみられる分げつ型の特徴について"近畿作物育種研究. 45. 39-41 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuda, K.: "Molecular identification of Echinochloa oryzicola and E. crus-galli using a PCR-RFLP technique"Weed Biology and Management. 2・1. 11-17 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamaguchi, H.: "Establishment of standard experimental strain tagging by microsatellites in weedy Echinochloa"Proc. 19th APWSS Conf.. I. 221-226 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Michishita, Y.: "Unique forms of weeds and millets in East Asian annual Echinochloa"Proc. 19th APWSS Conf.. I. 215-220 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 藪野友三郎: "ヒエという植物"全国農業教育協会. 203 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Umemoto, S.: "Immigration mode of Eclipta in Japan"Scientific Report of College of Agriculture, Osaka Prefecture University. 51. 25-31 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Umemoto, S.: "Is Poa crassinervis Honda endemic to Japan"Proceeding of 17 th APWSS Conference, Beijing. I(B). 610-613 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohe, M.: "A note on stem branching system in barnyard grasses"Kinki Jour. Crop Sci. Breed. 45. 39-41 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuda, K.: "Molecular identifiration of Echinochloa oryzicola and E. crus-galli using a PCR-RFLP technique."Weed Biology and Management. 2(1). 11-17 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamaguchi, H.: "Establishment of standard experimental strain tagging by microsatellites in weedy Echinochloa."Proc. 19th APWSS Conf.. I. 221-226 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Michishita, Y.: "Unique forms of weeds and millets in East Asian annual Echinochloa."Proc. 19th APWSS Conf.. I. 215-220 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yabuno, T.: "Natural history of barnyard grass"Zenkoku Nougyou Kyouiku Kyoukai Publ.. 203

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamaguchi H.: "Natural History of Millets"Hokkaido University Press. In press

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamaguchi, H.: "Establishment of standard experimental strain tagging by micro satellites in weedy Echinochloa"Proc. 19th Asian Pacific Weed Science Conference. I. 221-226 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Michishita, Y.: "Unique forms of weeds and millets in East Asian annual Echinochloa"Proc. 19th Asian Pacific Weed Science Conference. I. 215-220 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakayama, Y.: "Natural hybridization in wild soybean (Glycine max ssp. soja) by pollen flow from cultivated soybean (Glycine max ssp. max) in a designed population"Weed Biology and Management. 2(1). 25-30 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 石川 枝津子: "畑地雑草の生理・生態の変異の評価"育種作物学会北海道談話会会報. 42. 137-138 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 伊東 操子: "日本のゴルフ場におけるツルスズメノカタビラの侵入実態"雑草研究. 47・2. 82-88 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 石神真智子: "栽培および野生コブナグサにおける出穂と形態的変異"雑草研究. 46・3. 194-200 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasuda, K.: "Molecular identification of Echincochloa oryzicola and E.crus-galli using a PCR-RFLP technique"Weed Biology and Management. 2(1)(印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] 石川枝津子: "畑作、雑草の生理・生態の変異の評価"日本育種学会・日本作物学会北海道談話会誌. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] 山口裕文: "栽培植物の自然史"北海道大学図書刊行会. 241 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 大江真道: "ノビエにみられる分げつ型の特徴について"近畿作物育種研究. 45. 39-41 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mimura,M.: "RAPD variation of wild, weedy and cultivated azuki beans in Asia"Genetic Reseuices and Crop Evolution. 47. 603-610 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 藪野友三郎(監修): "増補改訂 ヒエの博物学"全国農村教育協会(印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] 山口裕文: "雑穀の自然史"北海道大学図書刊行会(印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Umemoto, S.: "Immigration mode of Eclipta into Japan"Scientific Report Colloge Agriculture, Osaka Prefecture university. 51. 25-31 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Umemoto, S.: "Is Poa crassiner vis Honda endemic to Japan?"Proc. 17th APWSS Conf.. I(B). 610-613 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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