Elucidation of the mechanism for posttranslational activation of RubisCO in prokaryotes and its application

• Project/Area Number: 11556013
• Research Category: Grant-in-Aid for Scientific Research (B).
• Allocation Type: Single-year Grants
• Section: 展開研究
• Research Field: 応用微生物学・応用生物化学
• Research Institution: THE UNIVERSITY OF TOKYO
• Principal Investigator: IGARASHI Yasuo Graduate school of agricultural and life sciences, THE UNIVERSITY OF TOKYO Professor, 大学院・農学生命科学研究科, 教授 (90114363)
• Co-Investigator(Kenkyū-buntansha): ISHII Masaharu Graduate school of agricultural and life sciences, THE UNIVERSITY OF TOKYO Associate Professor, 大学院・農学生命科学研究科, 助教授 (30193262)
• Project Period (FY): 1999 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Budget Amount: ¥13,000,000 (Direct Cost: ¥13,000,000); Fiscal Year 2000: ¥3,200,000 (Direct Cost: ¥3,200,000); Fiscal Year 1999: ¥9,800,000 (Direct Cost: ¥9,800,000)
• Keywords: RubisCO

Research Abstract

In order to clarify the mechanism of the posttranslational activation of RubisCO in prokaryotes, we used a thermophilic hydrogen bacterium, Hydrogenophilus thermoluteolus. And the following results were obtained.
(1) Gene product of cbbQ and/or cbbO.which locates downstream of the structural genes for RubisCO, is involved in the RubisCO activation.
(2) Through analysis of CD spectra, activation of RubisCO is accompanied by strautural change of the protein.
(3) CBBQ catalyzes ATP hydrolysis. The reaction required Mg^<2+> and was inhibited by ADP.The characteristics are similar to those known for RubisCO activase. In contrast that RubisCO activase hydrolyzes only ATP, CBBQ hydrolyzed not only ATP but also UTP, GTP, CTP, and TTP.
(4) Analysis of gene cluter for Calvin-Benson cycle was completed. The gene arrangement was clarified as follows ; cbbLSQOYA-pyk-cbbRFPTGK.

Research Products

• [Publications] Nobihiro R.Hayashi ら: "Structure of Ribulose 1,5- bisphosphate Carboxylase/ Oxygenase Gene Cluster from a Thermophilic Hydrogen- Oxidizing Bacterium, Hydrogenophilus thermoluteolus, and Phylogeny of the Fructose 1,6- Bisphosphate Aldolase Encoded by cbbA in the Cluster"Bioscience Biotechnology and Biochemistry. 64・1. 61-71 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nobuhiro R.Hayashi et al.: "Structure of Ribulose 1,5-bisphosphate Carboxylase/Oxygenase Gene Cluster from A Thermophilic Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus. and Phylogeny of the Fructose 1,6-Bisphosphate Aldolase Encode by cbbA in the Cluster"Bioscience Biotechnology and Biochemistry. 64-1. 61-71 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nobuhiro R.Hayashi ら: "Structure of Ribulose 1,5-bisphosphate Carboxylase/Oxygenase Gene Cluster from a Thermophilic Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus, and Phylogeny of the Fructose 1,6-Bisphosphate Aldolase Encoded by cbbA in the Cluster"Bioscience Biotechnology and Biochemistry. 64・1. 61-71 (2000)