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Screening of bio-active substances by using a peculiar phenotype of yeast mutant

Research Project

Project/Area Number 11556017
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 応用微生物学・応用生物化学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

MIYAKAWA Tokichi  Hiroshima University, Graduate School of Advances Sciences of Matter., Professor, 大学院・先端物質科学研究科, 教授 (10116676)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hidetoshi  Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., 生物科学研究所, 主任研究員
HIRATA Dai  Hiroshima University, Graduate School of Advances Sciences of Matter., Associate Professor, 大学院・先端物質科学研究科, 助教授 (30243603)
Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2000: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥4,400,000 (Direct Cost: ¥4,400,000)
KeywordsCa2+ signal / Calcineurin / Drug screening / MAP kinase / 生理活性物質 / 出芽酵母 / カルシニューリンニューリン / MAPキナーゼ / 細胞周期 / カルシウム / 免疫抑制剤
Research Abstract

An inappropriate activation of a signaling pathway in yeast often has a deleterious physiological effect and causes various defects, including growth defects. In a certain genetic background (Δzdsl) of Saccharomyces cerevisiae, the cell-cycle progression in G2 is specifically blocked in the medium with CaCl_2 by the hyperactivation of the Ca^<2+>-signaling pathways. Here, we developed a novel drug screening procedure designed to detect the active compounds that specifically attenuate the Ca^<2+>-signaling activity on the basis of the ability to abrogate the growth defect of the cells suffering from the hyperactivated Ca^<2+> signal. Using known calcineurin inhibitors as model compounds, we have established the screening conditions for the drugs that suppress the Ca^<2+>-induced growth inhibition. An indicator strain with an increased drug sensitivity was constructed with a syr1/erg3 null mutation.

Report

(5 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (49 results)

All Other

All Publications (49 results)

  • [Publications] Yuji Miyamoto et al.: "Identification of Saccharomyces cerevisiae isoeucyl tRNA synthetase as target of G_1-specific inhibitor reveromycin A"J. Biol. Chem.. 277. 28810-28814 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Dai Hirata et al.: "Fission yeast Mor2/Cps12, a protein similar to Drosophila Furry, is essential for cell morophogenesis and its mutation induces Weel-dependent G_2 delay"EMBO J.. 21. 4863-4874 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Daisuke Morita et al.: "Rpf2p, an evolutionarily conserved protein, interacts with ribosomal protein L11 and is essential for the processing of 27 SB pre-rRNA to 25 S rRNA"J. Biol. Chem.. 277. 28780-28786 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koji Kakugawa et al.: "Cloning, characterization, and expression of cDNA encoding a lipase from Kurtzumanomyces sp. I-11"Biosci. Biotechnol. Biochem.. 66. 1328-1336 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koji Kakugawa et al.: "Purification and characterization of a lipase from the glycolipid-producing yeast Kurtzumanomyces sp. I-11"Biosci. Biotechnol. Biochem.. 66. 978-985 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koji Kakugawa et al.: "Isolation of yeast Kurtzumanomyces sp. I-11, novel producer of mannosyterythritol lipid"Biosci. Biotechnol. Biochem.. 66. 188-191 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 下向敦範 ほか: "清酒酵母の研究(分担執筆)"清酒酵母研究会. 8 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Miyamoto et al.: "Identification of Saccharomyces cerevisiae isoeucyl tRNA synthetase as target of G_1-specific inhibitor reveromycin A"J. Biol. Chem.. 277. 28810-28814 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] D. Hirata et al.: "Fission yeast Mor2/Cps12, a protein similar to Drosophila Furry, is essential for cell morophogenesh and its mutation induces Wee1-dependent G_2 delay"EMBO J.. 21. 4863-4874 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] D. Morita et al.: "Rpf2p, an evolutionary conserved protein, interacts with ribosomal protein L11 and is essential for the processing of 27 SB pre-rRNAto 25 S rRNA"J. Biol. Chem.. 277. 28780-28786 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K. Kakugawa et al.: "Cloning, characterization, and expression of cDNA encoding a lipase from Kurtzumanomyces sp. I-11"Biosci. Biotechnot. Biochem.. 66. 1328-1336 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K. Kakugawa et al.: "Purification and characterization of a lipase from the glycolipid-producing yeast Kurtzumanomyces sp, I-11"Biosci. Blotechnol. Biochem.. 66. 978-985 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K. Kakugawa et al.: "Isolation of yeast Kurtzumanomyces sp. I-11, novel producer of mannosylerythritol lipid"Biosci. Blotechnol. Biochem.. 66. 188-191 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaki Mizunuma et al.: "GSK-3 kinase Mck1 and calcineurin coordinately mediates Hsl1 down-regulation by Ca^<2+> in budding yeast."EMBO J.. 20. 1074-1085 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] X. Zhang et al.: "Crosstalk between transcriptipnal regulators in multidrug resistance in Saccharomyces cerevisiae"J. Biol. Chem.. 276. 8812-8819 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Osamu Tanabe et al.: "Fission yeast homologues of the B subunit of protein phosphatase 2A: multiple roles in mitotic cell division and functional interaction with calcineurin."Gene Cells. 6. 455-473 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tomonori Hosotani et al.: "PKC1, a protein kinase C homologue of Saccharomyces cerevisiae participates in microtubule function through the yeast EB1 homologue BIM1."Gene Cells. 6. 775-778 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Atsunori Shitamukai et al.: "A positive screening for drugs that specifically inhibit the Ca^<2+>-signaling activity on the basis of the growth promoting effect on a yeast mutant with a peculiar phenotype"Biosci. Biotechnol. Biochem.. 64. 1942-1946 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masako Suda et al.: "Regulation of Wee1 kinase in response to protein synthesis inhibition"FEBS Letters. 486. 305-309 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akiko Tsuno et al.: "RRS1, a conserved essential gene, encodes a novel regulatory protein required for ribosome biogenesis in Saccharomyces cerevisiae."Mol. Cell. Biol.. 20. 2066-2074 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Rota Tsujii et al.: "Ebp2, yeast homologue of a human protein that interacts with Epstein-Barr virus nuclear antigen 1, is required for pre-rRNA processing and ribosomal subunit assembly"Genes to Cells. 5. 543-553 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Zhifeng Cui et al.: "Functional analysis of the promoter of the yeast SNQ2 gene encording a multidrug resistance transporter thai confers the resistance to 4-Nitroquinoline N-Oxide,"Biosci, Biotechnol. Biochem.. 63. 162-167 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ileana Cornelia Farcasanu et al.: "Involvement of thioredoxin peroxidase typeII (Ahp1p) of Saccharomyces cerevisiae in Mn^<2+> homeostasis."Biosci. Biotechnol. Biochem.. 63. 1871-1881 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masako Suda et al.: "Overproduction of elongation factor 1a, an essential translational component, causes aberrant cell morphology by affecting the control of growth polarity in fission yeast"Gene Cells. 4. 517-527 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masashi Yukawa et al.: "Nps1/Sth1p, a component of an essential Saccharomyces cerevisiae, is required for the maximal expression of early meiotic genes."Gene Cells. 4. 99-110 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yuji Miyamoto et al.: "Identification of Saccharomyces cerevisiae isoeucyl tRNA synthetase as target of G-1 -specific inhibitor reveromycin A"J. Biol Chem.. 277. 28810-28814 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Dai Hirata et al.: "Fission yeast Mor2/Cps12, a protein similar to Drosophila Furry, is essential for cell morophogenesis and its mutation induces Wee1-dependent G_2 delay"EMBOJ.. 21. 4863-4874 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Daisuke Morita et al.: "Rpf2p, an evolutionarily conserved protein, interacts with ribosomal protein L11 and is essential for the processing of 27 SB pre-rRNA to 25 S rRNA"J. Biol. Chem.. 277. 28780-28786 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Koji Kakugawa et al.: "Cloning, characterization, and expression of cDNA encoding a lipase from Kurtzumanomyces sp. I-11"Biosci. Biotechnol. Biochem. 66. 1328-1336 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Koji Kakugawa et al.: "Purification and characterization of a lipase from the glycolipid-producing yeast Kurtzurnanomyces sp. I-11"Biosci. Biotecimol. Biochem.. 66. 978-985 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Koji Kakugawa et al.: "Isolation of yeast Kurtzumanomyces sp. I-11, novel producer of mannosylerythritol lipid"Biosci. Biotechnol. Biochem.. 66. 188-191 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 下向敦範 ほか: "清酒酵母の研究(分担執筆)"清酒酵母研究会. 8 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] X.Zhang et al.: "Crosstalk between transcriptional regulators in multidrug resistance in Sacccharomyces cerevisae"J. Biol. Chem.. 276. 8812-8819 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Osamu Tanabe et al.: "Fission yeast homologues of the B subunit of protein phosphatase 2A : multiple roles in mitotic cell division and functional interaction with calcineurin"Gene Cells. 6. 455-473 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomoki Hosotani et al.: "PKC1, a protein kinase C homologue of Saccharomyces cerevisiase, participates in microtubule function through the yeast EB1 homologue BIM1"Gene Cells. 6. 775-788 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Eiko Tsuchiya et al.: "Borrelidin inhibits a cyclin-dependent kinase (CDK), Cdc28/Cln2, of Saccharomyces cerevisiae"J. Antibiotics. 54. 84-90 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masaki Mizunuma et al.: "GSK-3 Kinase Mck1 calcineurin coorsinately mediate Hsl1 down-regulation by ca^<2+> in budding yeast"ENBO・J. 20. 1074-1085 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Shitamukai: "A positive screening for drugs that specifically inhibits the Ca^<2+> signaling acitivity on the basis of the growth promoting effect on a yeast mutant with a peculiar phenotype "Biosci.Biotechnol.Biocehm.. 64. 1942-1946 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.Tsuno: "RRS1 a conserved essential gene, encodes a novel regulatory protein required for ribosome biogenesis in Saccharomyces serevisiae"Mol.Cell.Biol.. 20. 2066-2074 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Suda: "Regulation of Weel kinase in response to protein synthesis inhibition"FEBS Letters. 486. 305-309 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Mizunuma: "GSK-3 kinase Mck1 and calcineurin coordinately mediate Hsl1 down regulation by Ca^<2+> in budding yeast"EMBO J.. 20(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Zhang,X.: "Crosstalk between transcriptional regulations of multidrug resistance in yeast"J.Biol.Chem.. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 水沼正樹: "Ca^<2+>シグナルによる細胞周期制御機構"実験医学. 18. 840-845 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 宮川都吉: "バイオサイエンスとバイオインダストリー"真核微生物の多剤耐性. 17-20 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Z. Cui: "Functional analysis of the yeast SNQ2 gene encoding a yeast multidrug resistancc transporter that corifers resistance to 4NQO"Biosci, Biotechnol. Biochem.. 63(1). 162-167 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] I, C, Farcasanu: "Involvement of Huoredoxine peroxidase typeII (Ahp1) of S. cerevisial in Mn^<2+> homeostasis"Biosci. Biotechnol. Biochem.. 63(11). 1871-1881 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Suda: "Overproduction of EFId, an esseutial translational couponeut, causes abbenant cell morpho logy in yeast"Genes to Cells. 4. 517-527 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Yukawa: "Nps1/sth1, a componeut of chromatin-remodeling complex of S. cerenisial, is required for meiotic genes"Genes to Cells. 4. 99-110 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 平田大: "新規の細胞周期チェックポイント制御とMAPオナーゼ"実験医学. 17. 124-129 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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