| Project/Area Number |
11556030
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
林産学
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| Research Institution | TOKYO UNIVERSITY OF AGRICULTURE & TECHNOLOGY |
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Principal Investigator |
KATAYAMA Yoshihiro TOKYO UNIVERSITY OF AGRICULUTURE & TECHNOLOGY, GRADUATE SCHOOL OF BIO-APPLICATIONS & SYSTEM ENGINEERING, SYSTEM ENGINEERING, 大学院・生物システム応用科学研究科, 助教授 (10214339)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIKAWA Seiji COSMO OIL CO., LTD., RESEARCH & DEVELOPMENT CENTER, MANAGER SPECIALTY CHEMICAL GROUP, 中央研究所, (研究職)グループ長
SHIGEHARA Kiyotaka TOKYO UNIVERSITY OF AGRICULUTURE & TECHNOLOGY, GRADUATE SCHOOL OF BIO-APPLICATIONS & SYSTEM ENGINEERING, SYSTEM ENGINEERING, 工学部, 教授 (60170867)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2000: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | liignin / biotransformation / Sptiingomoas paucimobilis SYK-6. / genelJc engineering / 2-Pyrone-4,6-dicarboxylicacid / bio-reactor / polyamidetype polymer / interfacialpolymerizatiou reaction / 2-ピロン4, 6-ジカルボン酸 / バイオリアクター / 2-ピロン4,6-ジカルボン酸 |
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| Research Abstract |
This project has been investigating the biotransformation of lignin based on the catabolism of ligninolytic bacterium Sphing omonas paucimobilis SYK-6. 2-Pyroae-4 , 6-dicarboxylicacid (PDC) is a major and characteristic intermediate of protocatecuate meta cleavage pathway of Sphinomonas paucimobilis SYK-6 in lignin degradation. We have preparedthe new recombinant plasmid vector which was based on the Escherichia coli.-Pseudomonas shutU.5 vector PKT-230 and composed of Lig A, B genes (protocatechuate 4,4-dioxygenase gene), the modified VanA, B gene (Vanillate, Syringate demethylase gene) and Lig C gene (PDC synthase). In this project research, we have succeeded in producing PDC in the high yield (ca.90%) from the low-molecular weight lignin by the transformant of Pseodoaiowsputicla PpYllOO having the new reconbinantplasmid vector.
Since PDC has the unique molecular structure and the polymer made froin PDC is expectedto have the new function as optical material, we prepared new polyamide type polymer material by the inteifacial polymerizationreaction with hexamethylenediamine.
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