| Project/Area Number |
11556046
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
生物環境
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
FUJIWARA Kazuhiro Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (30211535)
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| Co-Investigator(Kenkyū-buntansha) |
YANO Akira Shimane Univ. Faculty of Life and Environ. Science, Associate Professor, 生物資源科学部, 助教授 (70292670)
KANEKO Keiko Graduate School of Agric. and Life Sciences, Research Assistant, 大学院・農学生命科学研究科, 助手 (50332599)
IBARAKI Yasuomi Yamaguchi Uni., Faculty of Agriculture, Associate Professor, 農学部, 助教授 (50242160)
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| Project Period (FY) |
1999 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1999: ¥6,600,000 (Direct Cost: ¥6,600,000)
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| Keywords | active oxygen / cartenoid / chemiluminescence / electrolyzed-reduced water / electrolyzed water / ESR / light quality / photoinhibition / 青色光 / キサントフィル / β-カロテン / 活性酵素 / 光質(光波長組成) / 過酸化水素 / luminol / 陰極水 / サラダナ / 電気分解 / 水 / 機能水 / 光合成量子収率 / 電子スピン共鳴法 |
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| Research Abstract |
The objective of this project was to develop an irrigation water treatment methods and/or crop management methods which could reduce photoinhibition of crop photosynthesis. Shirahata et al. (1997) reported that electrolyzed-reduced water had a power to scavenge active oxygen. Based on this report and an established knowledge that photoinhibition was caused by active oxygen, we made a working hypothesis : photoinhibition of crops could be reduced by giving them electrolyzed-reduced water. To verify this working hypothesis, we applied electrolyzed-reduced water to protoplasts from sweetpotato and determined the degree of photoinhibition by chlorophyll fluorescence (Mini-PAM) and also measured production rate of O2^- by luminescence from lucigenin. The results obtained did not support the working hypothesis. A measurement of O2^- scavenging power of electrolyzed-reduced water by Cytochrome C method did not support the hypothesis, either. Measurement of O^2- by ESR resulted in higher O2^- content in electrolyzed-reduced water compared to pure water.
High cartenoid content in leaves is known to have a high ability of suppressing active oxygen production. Therefore, we investigated the possibility of reducing photoinhibition by controlling light quality (spectrum) applied to crops. We compared cartenoid contents in leaves of Komatsuna (Brassica campestris L) grown under blue, red and white fluorescence lamps. The results indicate that red light increased cartenoid content, compared to the other lights.
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