| Project/Area Number |
11556055
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied animal science
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
KUNIEDA Tetsuo OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF NATURAL SCIENCE AND TECHNOLOGY, PROFESSOR, 大学院・自然科学研究科, 教授 (80178011)
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| Co-Investigator(Kenkyū-buntansha) |
MORITOMO Yasuo KYUSYU TOKAI UNIVERSITY, FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (70148972)
OIKAWA Takuro OKAYAMA UNIVERSITY, FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (90160407)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 2000: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1999: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | CHONDRODYSPLASA / JAPANESE BROWN CATTLE / COMPARATIVE MAPPING / HEREDITARY DISEASE / DNA DIAGNOSTIC SYSTEM / LINKAGE MAPPING / RH MAPPING |
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| Research Abstract |
The aim of the present study is to identify the gene responsible for bovine chondrodysplatic dwarfism and establish the DNA diagnosis system for the disease. A hereditary chondrodysplastic dwarfism has been reported in the particular population of Japanese Brown cattle. Affected animal shows an insufficiency of endochondral ossification at long bones of the limbs and no other associated changes. An autosomal recessive trait of inheritance has been revealed by investigating pedigrees of the affected animals.
In the present study, we mapped the locus responsible for the disease by linkage analysis using microsatellite markers and a single paternal half-sib family (n=82) of affected animals obtained from commercial herds. The linkage analysis revealed a significant linkage between the locus, referred as bcd, and marker loci on bovine Chromosome 6. The bcd locus was mapped between microsatellite markers BP7 and BM9257. A comparison of genetic maps between bovine and human indicates that the majority of bovine chromosome 6 is homologous to the segment of human chromosome 4 between 4p16.3 to 4q25. The segment involves several candidate genes including FGFR3 gene which is responsible for human achondroplasia. To determine exact relation between bovine chromosome 6 and human chromosome 4, we performed radiation hybrid mapping using the bovine hamster radiation hybrid panel. Consequently, a comprehensive radiation hybrid map of bovine chromosome 6 consisting with 33 functional genes and 32 microsatellite markers was constructed. A comparison between the radiation hybrid map of bovine chromosome 6 and human chromosome 4 revealed the region of human chromosome 4 corresponding to the region of bovine chromosome 6 in which the bcd locus is localized.
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