| Project/Area Number |
11556065
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Institute of Molecular and Cellular Biosciences, The University of Tokyo |
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Principal Investigator |
KATO Shigeaki Institute of Molecular and Cellular Biosciences, The University of Tokyo Professor, 分子細胞生物学研究所, 教授 (60204468)
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| Co-Investigator(Kenkyū-buntansha) |
SHIKAMA Hisataka Metabolic Deseases Research, Pharmacology Laboratories, Yamanouchi Pharamaceutical Co., Ltd. Manager, 薬理研究所・薬理第三研究室, 室長(研究職)
YANAGISAWA Junn Assistant Professor, 分子細胞生物学研究所, 助手 (50301114)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2000: ¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1999: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | sex steroid recepter / cofactors / in vitro interaction / transactivation / hormone-like compound / yeast two-hybrid assay / receptor cofactor / cDNA screening / ホルモン関連化合物 / ステロイドホルモンレセプター / 共役転写因子群 / 転写促進領域 / ERα・ERβ / AR / クロマチン構造 |
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| Research Abstract |
Steroid hormones exert their actons through cognate nuclear receptors, which act ligand-inducible transcriptional factors. For this ligand-induced transactivation, ligand-bound nuclear receptors, nuclear receptors depend on ligand binding, but it is induced only by agonist, but not antgonist. In the present study, we searched novel coactivators for nuclear receptors for estrogen, androgen mineralcorticoid, vitamin D by biochemical approaches and yeast two-hybrid system, and found several candidate cDNAs. Especially, we found that p68/p72 acts as a co-activator specific for ERα. Moreover, we developed an assay system based on the interactions of coactivators with nuclear receptors in yeast to evaluate agonistic/antagonistid activity of related compounds.
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