| Project/Area Number |
11557016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Human pathology
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| Research Institution | SAGA MEDICAL SCHOOL (2000-2001)
University of Tsukuba (1999) |
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Principal Investigator |
WATANABE Teruo SAGA MEDICAL SCHOOL, VICE PRESIDENT, 副学長 (40037396)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Kazuo YAMANOUCHI TSUKUBA RESEARCH INSTITUTE, PRESIDENT, 筑波研究所, 薬理研究所長
FAN Jianglin UNIVERSITY OF TSUKUBA, INSTITUTE OF BASIC MEDICAL SCIENCES, LECTURER, 基礎医学系, 講師 (60272192)
KIMOTO Masao SAGA MED SCHOOL, IMMUNOLOY, PROFESSOR, 医学部, 教授 (40153225)
井出 良浩 筑波大学, 基礎医学系, 講師 (70258606)
長崎 比呂央 明治大学, 農学部, 助教授 (50318664)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2000: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1999: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | clone / animal cloning / hypercholesterolemia / atherosclerosis / animal models / transgenic rabbit / transgene / lipoproteins / 遺伝子操作 / ウサギ / 体細胞クローン / 細胞核融合 |
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| Research Abstract |
Transgenic rabbits are widely used as both bioreactors for the production of therapeutic proteins and human disease models in many biomedical fields. In attempt to generate transgenic rabbits by nuclear transfer technique, we performed the initial trial in making clone rabbits using somatic cells from transgenic rabbits and herewith we have optimized several factors that may be involved in the success of nuclear transfer procedures such as culture media, fusion and activation conditions and other related factors. Both electric fusion and piezo methods were used in this study. Although the generation of clone rabbits using somatic cells is still in immature state, it seems that compared to other species (goat, sheep, cow, mouse and pig), rabbit cloning using somatic cell nuclear transfer is rather complicated and a lot of procedures need to be refined before we can succeed in making cloned rabbits. In the second part of the study, we investigated transgenic rabbits expressing human apo(a) in terms of Lp(a) atherogenicity after cholesterol diet feeding. We found that transgenic rabbits developed greater atherosclerosis in aorta and coronary arteries than did control rabbits.
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