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Analysis of molecular mechanism of premalignant lesion of the stomach using genomic instability and new mucin gene.

Research Project

Project/Area Number 11557043
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field Gastroenterology
Research InstitutionSapporo Medical University

Principal Investigator

IMAI Kohzoh  Sapporo Medical University, School of Medicine First Department of Internal Medicine, Professor, 医学部, 教授 (60117603)

Co-Investigator(Kenkyū-buntansha) HINODA Yuji  Yamaguchi University, School of Medicine Department of Clinical Laboratory Medicine, Professor, 医学部, 教授 (10165128)
ITOH Fumio  Sapporo Medical University, School of Medicine First Department of Internal Medicine, Assistant Professor, 医学部, 講師 (90223180)
Project Period (FY) 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 2000: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1999: ¥5,400,000 (Direct Cost: ¥5,400,000)
Keywordsgastric pre-cancerous lesions / genomic instability / DNA repair genes / mucin genes / methylation / メチレ-ション
Research Abstract

1) High frequency of the genomic instability (microsatellite instability, MSI) was found in well-differentiated adenocarcinoma of the stomach. Most of the intestinal metaplasia lesion which was accompanied with well-differentiated adenocarcinoma showed positivity for MSI.Moveover, hypermethylation of the promotor lesion of hMLH1 gene was observed in MSI-positive adenocarcinoma. Further study will be needed to analyze the association between MSI status and hypermethylation of the DNA repair genes and other related genes.
2) New mucin gene A3D4 was expressed in the lesion of intestinal metaplasia of the adenocarcinoma of the stomach. This product was negative for "normal" intestinal metaplasia lesion.
3) Methylation has systemically been investigated in the lesion of the intestinal metaplasia and many genes were found to show abnormal pattern of methylation, suggesting the important role of methylation to the premalignant status of the stomach cancer.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Yamamoto H,Imai K. et al.: "Somatic mutation of the β2-microglubulin gene associated with untavorable prognosis in gastrointestinal cancer of the microsatellite mutator pbenotype"Gastroenterology. 120(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamamoto H,Imai K, et al.: "Genetic and clinical features of human pancreatic ductal adenocarcinomas with widespread microsatellite instability."Cancer Res. 61(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takekawa M,Imai K. et al.: "p53-inducible Wip1 phosphatase mediates a negative feedback regulation of p38 MAPK-p53 signaling in response to UV rediateion."EMBO J. 19. 6517-6526 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki H,Imai K. et al.: "Inactivation of the 14-3-3 sigma gene is associated with 5' CpG island hypermethylation in human cancers."Cancer Res. 60. 4353-4357 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shirakawa K,Imai K. et al.: "Gene targeting Flt-1 and Tie 2 inhibits tumor growth and metastases on WIBC-9, a human inflammatory cancer xenograft."Cancer Res. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naishiro Y.Imai K. et al.: "Restroration of epithelial cell polarity in a colorectal cancer cell line by suppression of β-catenin/T cell factor 4-mediated gene transactivation."Cancer Res. 61(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamamoto H, Itoh F, Imai K, et al.: "Somatic mutation of the β2-microglubulin gene associated with unfavorable prognosis in gastrointestinal cancer of the microsatellite mutator phenotype."Gastroenterology. 120 : (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamamoto H, Itoh F, Imai K, et al.: "Genetic and clinical features of human pancreatic ductal adenocarcinomas with widespread microsatellite instability."Cancer Res. 61 : (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takekawa H, Itoh F, Imai K, et al.: "p53-inducible Wip1 phosphatase mediates a negative feedback regulation of p38 MAPK-p53 signaling in response to UV rediation."EMBO J. 19. 6517-6526 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki H, Itoh F, Imai K, et al.: "Inactivation of the 14-3-3 sigma gene is associated with 5' CpG island hypermethylation in human cancers."Cancer Res. 60. 4353-4357 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shirakawa K, Imai K, et al.: "gene targeting Flt-1 and Tie 2 inhibits tumor growth and metastases on WIBC-9, a human inflammatory cancer xenograft."Cancer Res. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naishiro Y, Imai K, et al.: "Restroration of epithelial cell polarity in a colorectal cancer cell line by suppression of β-catenin/T cell factor 4-mediated gene transactivation."Cancer Res. 61 : (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamamoto H,Imai K. et al.: "Somatic mutation of the β2-microglubulin gene associated with untavorable prognosis in gastrointestinal cancer of the microsatellite mutator phenotype"Gastroenterology. 120(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamamoto H,Imai K. et al.: "Genetic and clinical features of human pancreatic ductal adenocarcinomas with widespread microsatellite instability."Cancer Res. 61(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takekawa M,Imai K. et al.: "p53-inducible Wip1 phosphatase mediates a negative feedback regulation of p38 MAPK-p53 signaling in response to UV rediateion."EMBO J. 19. 6517-6526 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Suzuki H,Imai K. et al.: "Inactivation of the 14-3-3 sigma gene is associated with 5' CpG island hypermethylation in human cancers."Cancer Res. 60. 4353-4357 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shirakawa K,Imai K. et al.: "Gene targeting Flt-1 and Tie 2 inhibits tumor growth and metastases on WIBC-9, a human inflammatory cancer xenograft."Cancer Res. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Naishiro Y,Imai K. et al.: "Restroration of epithelial cell polarity in a colorectal cancer cell line by suppression of β-catenin/T cellfactor 4-mediated gene transactivation."Cancer Res. 61(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamamoto,H.,Imai K.et al.: "Gastric cancers of the microsatellite mutator phenotype display characteristic genetic and clinical features."Gastroenterology. 116. 1348-1357 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Naishiro,Y.,Imai K.et al.: "BAG-1 accelerates cell motility of human gastric cancer cells."Oncogene. 18. 3244-3251 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamamoto,H.,Imai K.et al.: "Overexpression of COX-2 protein is less freguent in gastric cancers with microsatellite instability."Int.J.Cancer. 84. 400-403 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toyota,M.,Imai,K.et al.: "Aberrant methylation in gastric cancer associated with a CpG island mathylator phenotype."Oncogene. 59. 5438-5442 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Okuda,H.,Imai,K.et al.: "Protein kinase Cα promotes apoptotic cell death in gastric cancer cells depending upon loss of anchorage."Oncogene. 18. 5604-5610 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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