| Project/Area Number |
11557058
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | KURUME UNIVERSITY |
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Principal Investigator |
MUROHARA Toyoaki THE CARDIOVASCULAR RESEARCH INSTITUTE, KURUME UNIVERSITY, ASSISTANT PROFESSOR, 循環器病研究所, 講師 (90299503)
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| Co-Investigator(Kenkyū-buntansha) |
KATOH Atsushi DEPARTMENT OF INTERNAL MEDICINE III, KURUME UNIVERSITY SCHOOL OF MEDICINE ASSISTANT PROFESSOR, 医学部, 助手 (70279165)
IKEDA Hisao DEPARTMENT OF INTERNAL MEDICINE III, KURUME UNIVERSITY, SCHOOL OF MEDICINE ASSOCIATE PROFESSOR, 医学部, 助教授 (50168134)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2000: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1999: ¥6,800,000 (Direct Cost: ¥6,800,000)
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| Keywords | ENDOTHELIAL PROGENITOR CELLS / ISCHEMIC HEART DISEASE / VASCULAR REGENERATION / PERIPHERAL ARTERY OCCLUSIVE DISEASE / VASCULOGENESIS / ANGIOGENESIS / ENDOTHELIAL CELL / COLLATERAL VESSEL / 再生医学 / 幹細胞 / 動脈硬化 / 臍帯血 / 成人末梢血 |
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| Research Abstract |
Endothelial precursor cells (EPCs) have been identified in adult peripheral blood. We examined whether EPCs could be isolated from Umbilical cord blood, a rich source for hematopoietic progenitors, and whether in vivo transplantation of EPCs could modulate postnatal neovascularization. Numerous cell clusters, spindle-shaped and attaching (AT) cells, and cord-like structures developed from culture of cord blood mononuclear cells (MNCs). Fluorescence-trace experiments revealed that cell clusters, AT cells and cord-like structures predominantly derived from CD34-positive MNCs (MNC^<CD34+>). Greater numbers of AT cells and cell clusters developed from cord blood-MNCs than from an equal amount of adult peripheral blood-MNCs. AT cells incorporated acetylated-LDL, released nitric oxide, and expressed KDR, VE-cadherin, CD31, and von Willebrand factor but not CD45. Locally transplanted AT cells survived and participated in capillary networks in the ischemic tissues of immunodeficient nude rats in vivo. AT cells thus had multiple endothelial phenotypes and were defined as a major population of EPCs. Furthermore, laser Doppler and immunohistochemical analyses revealed that EPC transplantation quantitatively augmented neovascularization and blood flow in the ischemic hindlimb. In conclusion, umbilical cord blood is a precious source for isolating EPCs, and transplantation of cord blood-derived EPCs would be a novel strategy to modulate postnatal neovascularization.
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