• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

ISOLATION OF ENDOTHELIAL PROGENITOR CELL FROM HUMAN UMBILICAL CORD BLOOD

Research Project

Project/Area Number 11557058
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field Circulatory organs internal medicine
Research InstitutionKURUME UNIVERSITY

Principal Investigator

MUROHARA Toyoaki  THE CARDIOVASCULAR RESEARCH INSTITUTE, KURUME UNIVERSITY, ASSISTANT PROFESSOR, 循環器病研究所, 講師 (90299503)

Co-Investigator(Kenkyū-buntansha) KATOH Atsushi  DEPARTMENT OF INTERNAL MEDICINE III, KURUME UNIVERSITY SCHOOL OF MEDICINE ASSISTANT PROFESSOR, 医学部, 助手 (70279165)
IKEDA Hisao  DEPARTMENT OF INTERNAL MEDICINE III, KURUME UNIVERSITY, SCHOOL OF MEDICINE ASSOCIATE PROFESSOR, 医学部, 助教授 (50168134)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2000: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1999: ¥6,800,000 (Direct Cost: ¥6,800,000)
KeywordsENDOTHELIAL PROGENITOR CELLS / ISCHEMIC HEART DISEASE / VASCULAR REGENERATION / PERIPHERAL ARTERY OCCLUSIVE DISEASE / VASCULOGENESIS / ANGIOGENESIS / ENDOTHELIAL CELL / COLLATERAL VESSEL / 再生医学 / 幹細胞 / 動脈硬化 / 臍帯血 / 成人末梢血
Research Abstract

Endothelial precursor cells (EPCs) have been identified in adult peripheral blood. We examined whether EPCs could be isolated from Umbilical cord blood, a rich source for hematopoietic progenitors, and whether in vivo transplantation of EPCs could modulate postnatal neovascularization. Numerous cell clusters, spindle-shaped and attaching (AT) cells, and cord-like structures developed from culture of cord blood mononuclear cells (MNCs). Fluorescence-trace experiments revealed that cell clusters, AT cells and cord-like structures predominantly derived from CD34-positive MNCs (MNC^<CD34+>). Greater numbers of AT cells and cell clusters developed from cord blood-MNCs than from an equal amount of adult peripheral blood-MNCs. AT cells incorporated acetylated-LDL, released nitric oxide, and expressed KDR, VE-cadherin, CD31, and von Willebrand factor but not CD45. Locally transplanted AT cells survived and participated in capillary networks in the ischemic tissues of immunodeficient nude rats in vivo. AT cells thus had multiple endothelial phenotypes and were defined as a major population of EPCs. Furthermore, laser Doppler and immunohistochemical analyses revealed that EPC transplantation quantitatively augmented neovascularization and blood flow in the ischemic hindlimb. In conclusion, umbilical cord blood is a precious source for isolating EPCs, and transplantation of cord blood-derived EPCs would be a novel strategy to modulate postnatal neovascularization.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Murohara T. et al.: "Transplanted cord blood-derived endothelial precursor cells augment postnatal neovascularization."J Clin Invest.. 105. 1527-1536 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duan J,Murohara T. et al.: "Hypercholesterolemia inhibits angiogenesis in response to hindlimb ischemia : nitric oxide-dependent mechanism."Circulation. 102. III-370-III-376 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duan J,Murohara T, et al.: "Hyperhomocysteinemia impairs angiogenesis in response to hindlimb ishemia."Arterioscler Thromb Vase Biol.. 20. 2579-2585 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ikeda H,Takajo Y,Murohara T. et al.: "Platelet-derived nitric oxide and coronary risk factors."Hypertension. 35. 904-907 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shintani S,Murohara T, et al.: "Augmentation of postnatal neovascularization with autologous bone marrow transplantation."Circulation. 103. 897-903 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Urano H,Murohara T, et al.: "Enhanced external counterpulsation improves exercise tolerance, reduces exercise-induced myocardial ischemia and improves left ventricular diastolic filling in patients with coronary artery disease."J Am Coll Cardiol.. 37. 93-99 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ikeda H, Takajo Y, Murohara T, Ichiki K, Adachi H, Haramaki N, Katoh A, Imaizumi T.: "Platelet-derived nitric oxide and coronary risk factors"Hypertension. 35. 904-907 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Murohara T, Ikeda H, Duan J, Shintani S, Sasaki Ki, Eguchi H, Onitsuka I, Matsui K, Imaizumi T.: "Transplanted cord blood-derived endothelial precursor cells augment postnatal neovascularization."J Clin Invest. 105. 1527-1536 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duan J, Murohara T, Ikeda H, Katoh A, Shintani S, Sasaki K, Kawata H, Yamamoto N, Imaizumi T.: "Hypercholesterolemia inhibits angiogenesis in response to hindlimb ischemia : nitric oxide-dependent mechanism."Circulation. 102 (Suppl III). III-370-III376 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Duan J, Murohara T, Ikeda H, Sasaki Ki, Shintani S, Akita T, Shimada T, Imaizumi T.: "Hyperhomocysteinemia impairs angiogenesis in response to hindlimb ischemia."Arterioscler Thromb Vasc Biol. 20. 2579-2585 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Urano H, Ikeda H, Ueno T, Matsumoto T, Murohara T, Imaizumi T.: "Enhanced external counterpulsation improves exercise tolerance, reduces exercise-induced myocardial ischemia and improves left ventricular diastolic filling in patients with coronary artery disease."J Am Coll Cardiol. 37. 93-99 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shintani S, Murohara T, Ikeda H, Ueno T, Sasaki Ki, Duan J, Imaizumi T.: "Augmentation of postnatal neovascularization with autologous bone marrow transplantation."Circulation. 103. 897-903 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takajo Y, Ikeda H, Haramaki N, Murohara T, Imaizumi T.: "Augmented oxidative stress of platelets in chronic smokers. Mechanisms of impaired platelet-derived nitric oxide bioactivity and augmented platelet aggregability."J Am Coll Cardiol. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Murohara T, Asahara T.: "Nitric oxide and angiogenesis in cardiovascular disease."Antioxi. Redox Signal. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Rivard A,Murohara T et al.: "Age-dependent impairment of angiogenesis."Circulation. 99. 111-120 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Eguchi H,Ikeda H,Murohara T,et al.: "Endothelial injuries of coronary arteries distal to thrombotic sites: Role of adhesive interaciton between endothlial P-selectin and leukocyte sialyl Lewis^X."Circ.Res.. 84. 525-535 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ikeda H,Ueyama T,Murohara T,et al.: "Adhesive interaction between P-selectin and sialyl lewisX plays an important role in recurrent coronary arterial thrombosis in dogs."Arterioscler.Thromb.Vasc.Biol.. 19. 1083-1090 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Murohara T,et al.: "Role of endothelial nitric oxide synthase in endothelial cell migration."Arterioscler.Thromb.Vasc.Biol.. 19. 1156-1161 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Murohara T,et al.: "Effects of atrial and brain natriuretic peptides on lysophosphatidylcholine-mediated endothelial dysfunction."J.Cardiovasc.Pharmacol.. 34. 870-878 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Murohara,T.,et al.: "Rescue of hypercholesterolemia-indused impairment of angiogenesis by dietary L-arginine."Circulation. 100. I-319 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 室原豊明、池田久雄、今泉 勉: "医学のあゆみ"医歯薬出版株式会社. (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 新谷 理、室原豊明: "医学のあゆみ"医歯薬出版株式会社. (2000)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi