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Development of a new photo-aging model system: A quick evaluation method for photo-damage utilizing elastin-promoter/green fluorescence protein (GFP) reporter gene transfected dermal fibroblasts

Research Project

Project/Area Number 11557062
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Dermatology
Research InstitutionHirosaki University

Principal Investigator

KON Atsushi  Hirosaki University School of Medicine, Dermatology, Assistant Professor, 医学部・附属病院, 講師 (60271798)

Co-Investigator(Kenkyū-buntansha) TAMAI Katsuto  Hirosaki University School of Medicine, Dermatology, Associate Professor, 医学部, 助教授 (20236730)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsphoto-aging / elastin promoter / solr elaslosis / ultraviolet / fibroblast / sunscreen
Research Abstract

For the purpose of prevention of photo-aging such as deep wrinkle formation, a great number of sunscreens against broad-band spectrum of solar ultraviolet (UV) has been produced. However, in vivo evaluation of sunscreens for anti-photoaging requires long time. In the present experiments, a new evaluation system have been developed. Cultured mouse fibroblasts (3T3) were transfected with human-elastin promoter linked to green fluorescence protein (GFP) reporter gene. After exposure to UVB, green fluorescence intensity of GFP was observed. In addition, after injection of the cells into the mouse skin, fluorescence intensity was quantified using spectrofluorometer with fiber optics (the SPEX FluoroMax-3 of Jobin Yvon Inc.). The cultured cells expressed green fluorescence in UVB-dose dependent manner. The fluorescence of injected cells was measurable from skin surface. These results indicate that quantitative measurement of fluorescence intensity of injected cells to the mice skin might be useful for early evaluation of solar elastosis formation and protective effect of sunscreens.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Hashimoto I, Kon A, Tamai K, Uitto J: "Diagnostic dilemma of "sporadic" cases of dystrophic epidermolysis bullosa : a new dominant or mitis recessive mutation?"Exp Deimatol. 8. 140-142 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tamai K, Murai T, Mayama M, Kon A, Nomura K, Sawamura D, Hanada K, Hashimoto I, Shimizu H, Masunaga T, Nishikawa T, Mitsuhashi Y, Ishida-Yamamoto A, Ikeda S, Ogawa H, McGrath JA, Pulkkinen L, Uino J: "Recurrent COL7AI mutations in Japanese patients with dystophic epidermolysis bullosa : Positional effects of premature termination codon mutations on clinical severity"J Invest Deimatol. 112. 991-993 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sawamura D, Ina S, Itai K, Meng X, Kon A, Tamai K, Hanada K, Hashimoto I: "In vivo gene introduction into keratinocytes using jet injection"Gene Ther. 6. 1785-1787 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tamai K, Murai T, Mayama M, Kon A, Sawamura D, Nomura K, Hanada K, Hashimoto I, Uitto J: "Recent seudies on DNA-based diagnosis for Japanese case of dystrophic epidermolysis bullosa"Molecular Medicine : Novel Findings of Gene Diagnosis, Regulation of Gene Expression, and Gene Therapy. 53-61 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kon A, Vindevoghel L, Hashimoto I, Uitto J, Mauviel A: "TGF-β/Smad and TNF-a /NF-KB upregulate type VII collagen gene expression additively at the transcriptional level"Molecular Medicine: Novel Findings of Gene Diagnosis, Regulation of Gene Expression, and Gene Therapy. 175-183 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sawamura D, Xianmin M, Ina s, Kon A, Tamai K, Ohe Y, Hashimoto I: "Expression vector with DNA of bovine papilloma virus 1 for keratinocyte gene therapy"J Dermatol Sci. 23. 111-116 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hashimoto I., Kon A., Tamai K., Uitto J.: "Diagnostic dilemma of "sporadic" cases of dystrophic epidermolysis bullosa: a new dominant or mitis recessive mutation?"Exp. Dermatol.. 8. 140-142 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tamai K., Murai T., Mayama M., Kon A., Nomura K., Sawamura D., Hanada K. Hashimoto I., Shimizu H., Masunaga T., Nishikawa T., Mitsuhashi Y., Ishida-Yamamoto A., Ikeda S., Ogawa H., McGrath JA., Pulkkinen .L, Uitto J.: "Recurrent COL7A1 mutations in Japanese patients with dystrophic epidermolysis bullosa-Positional effects of premature termination codon mutations on clinical severity"J. Invest. Dermatol.. 112. 991-993 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sawamura D., Ina S., Itai K., Meng X., Kon A., Tamai K., Hanada K., Hashimoto I.: "In vivo gene introduction into keratinocytes using jet injection"Gene Ther.. 6. 1785-1787 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murai T., Mayama M., Kon A., Sawamura D., Nomura K., Hanada K., Hashirnoto I., Uitto J.: "Recent studies on DNA-based diagnosis for Japanese case of dystrophic epidermolysis bullosa"Molecular Medicine: Novel Findings of Gene Diagnosis, Regulation of Gene Expression, and Gene Therapy. 53-61 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kon A., Vindevoghel L., Hashimoto I., Uitto J., Mauviel A.: "TGF-/β/Smad and TNF- α/NF-κB upregulate type VII collagen gene expression additively at the transcriptional level"Molecular Medicine: Novel Findings o Gene Diagnosis, Regulation of Gene Expression, and Gene Therapy. 175-183 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sawamura D., Xianmin M., Ina S., Kon A., Tamai K., Ohe Y., Hashimoto I.: "Expression vector with DNA of bovine papilloma virus 1 for keratinocyte gene therapy"J. Dermatol. Sci.. 23. 111-116 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kon A., Sawamura D., Itai K., Tamai K., Hashimoto I.: "Gene therapy of dystrophic epidermolysis bullosa by introduction of type VII collagen gone to keratinocytes"New direction for cellular and organ transplantation. 175-183 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary

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Published: 1999-04-01   Modified: 2016-04-21  

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