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ISOLATION AND STRUCTURE ANALYSIS OF SOLUBLE TRANSFERIN RECEPTOR FOR INTERNATIONAL ASSAY SYSTEM

Research Project

Project/Area Number 11557071
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Hematology
Research InstitutionASAHIKAWA MEDICAL COLLEGE

Principal Investigator

KOHGO Yutaka  ASAHIKAWA MEDICAL COLLEGE, DEPARTMENT OF MEDICINE, PROFESSOR, 医学部, 教授 (10133183)

Co-Investigator(Kenkyū-buntansha) UMEDA Mamoru  NISSUI PHARMACEUTICAL CO. LTD. CHIEF INVESTIGATOR, 研究本部, 主任研究員
TORIMOTO Yoshihiro  ASAHIKAWA MEDICAL COLLEGE, DEPARTMENT OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (00281882)
YAJIMA Hirofumi  SCIENCE UNIVERSITY OF TOKYO, DEPARTMENT OF APPLIED CHEMISTRY, ASSOCIATE PROFESSOR, 理学部, 助教授 (10147506)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsSOLUBLE TRANSFERR1N RECEPTOR / TRANSFERRIN / ANTI-TRANSFERR1N RECEPTOR ANTIBODY / HEMATOLOGIC DISEASE / LIVER DISEASE / RHEUMATOID ARTHRITIS / HFE / 可溶性トランスフェリン受容 / トランスフェリン受容
Research Abstract

The concentration of soluble transferrin receptors (sTfR) is widely recognized as a good serum marker reflecting the total erythropoirsis and intracellular iron levels of erythron. While, several ELISA kits to quantify serum sTfR have been developed, the values of scram sTfR are different among these EIJSA kite. These differences are mainly caused by the standard of sTfR and the mAbs to be used. Therefore, it is important to clarify the molecular form of native sTfR complex in serum for standardization of the sTfR assay. We elucidated the structure of purified sTfR complex obtained from human serum without a detergent and dissociation procedure by means of affinity chromolography using monoclonal anti-TfR antibody. The sTfR complex was considered to be composed of two sTfR and one transferrin (Tf) and the ratio of sTfR and Tf in the complex would change depend on the serum iron concentration and iron saturation of Tf. We then developed three monoclonal antibodies against different epitopes of TfR and investigated the antibody-reactivity against sTfR complex derived from hematopoietic disease (iron deficiency anemia: IDA) and non-hematopoielic disease (chronic hepatitis: CH and rheumatoid arthritis: RA) using the two sets of ELISA. The antigenesity of sTfR in CH and RA was different from that in IDA. Because the sTfR increased in CH and RA was derived from non-erythroid cell, sTfR structure was suggested to be different between erythroid and non-erythroid cells. These results suggest thatthe sTfR derived from erythroid cell has a specific structure and the establishment of sTfR assay system against this structure will be useful to evaluate the true erythropoiesis and iro status of erythron.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Ikuta K: "Overexpression of hemochromatosis p rotein, HFE, altcrs transferrin recycling process in human hepatoma cells"Biochim Biophys Acta. 1496・2-3. 221-231 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 鳥本悦宏: "トランスフェリン受容体と赤血球造血"臨床血液. 41. 554-558 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yajima H: "Molecular Modeling of Human Serum Transferrin for Rationalizing the Change in the Physicochemical Properties Induced by Iron Binding. Impication of the Mechanism of Biriding to Its Receotor"J. Protein Chem. 19. 215-223 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yajima H: "Small-Angle X-Ray Scattering Study of the Structure of Serum-Soluble Transferrin Receptor"PF Activity Rep.. 17. 304 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yajima H: "Small-Angle Neutron Scattering Study of the Structure of Serum-Soluble Transferrin Receptor with Contrast Variation Method"Activity Report on Neutron Scattering Research. 7. 264-265 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 高後 裕: "赤血球造血に関わる鉄代謝の腸管-肝臓-骨髄axis調節の分子機構"臨床血液. 42. 397-402 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kato J: "Ratio of transferrin (Tf) to Tf-receptor complex in circulation differs depending on Tfiron saturation"Clin Chem 48:2002. 48. 181-183 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikuta K. et al.: "Overxpfession of hemochromatosisis protein. HFE, alters transferrin recycling process in human hepatoma cells"Biohim. Biophys. Acta. 1496(2-3). 221-230 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Torimoto Y. et al.: "Transferrin receptor and etythropoiesis"Rinsho Keteueki. 41. 554-558 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yajima H. et al.: "Molecular Modeling of Human Serum Transferrin for Rationalizing the Change in the Physicochemical Properties induced by Iron Binding. Implication of the Mechanism of Binding to Its Receptor"J. Protein Chem.. 19. 215-223 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yajima H. et al: "Smail-Angle X-Ray Scattering Study of the Structure of Serum-Soluble Transfer Receptor"PF Activity Rep.. 17. 304-304 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yajima H. et al.: "Small-Angle Neutron Scattering Study of the Structure of Serum-Soluble Traasferrin Receptor with Contrast Variation Method"Activity Report on Neutron Scattering Research. 7. 264-265 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kohgo J. et al.: "Disorders of Iron Mctabolism involving Erythropoiesis - Molecular Mechanism of Gut-Liver-Bone Marrow Axis"Rinsho Ketsueki. 42. 397-402 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kato J. et al.: "Raatio of transferrin (Tf) to Tf-receptor complex in circulation differs depending on Tf iron saturation"Clin. Chem.. 48. 181-183 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikuta K, Fujimoto Y, Suzuki Y, Tanaka K, Saito H, Ohhira M, Sasaki K, Kohgo Y: "Overexpression of hemochromatosis protein, HFE, alters transferrin recycling process in human hepatoma cells"Biochim Biophys Acta. 1496・2-3. 221-231 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 鳥本悦宏, 高後 裕: "トランスフェリン受容体と赤血球造血"臨床血液. 41. 554-558 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yajima H, Sakaziri T, Kikuchi T, Morita M, Ishii T: "Molecular Modeling of Human Serum Transferrin for Rationalizing the Change in the Physicochemical Properties Induced by Iron Binding. Impication of the Mechanism of Binding to Its Receptor"J. Protein Chem.. 19. 215-223 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yajima H, Tanaka H, Sakajiri T, Ishii T, Umeda M, Kohgo Y: "Small-Angle X-Ray Scattering Study of the Structure of Serum-Soluble Transferrin Receptor"PF Activity Rep.. 17. 304 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yajima H, Tanaka H, Sakajiri T, Ishii T, Umeda M, Kohgo Y: "Small-Angle Neutron Scattering Study of the Structure of Serum-Soluble Transferrin Receptor with Contrast Variation Method"Activity Report on Neutron Scattering Research. 7. 264-265 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 高後 裕, 斉藤浩之: "赤血球造血に関わる鉄代謝の腸管・肝臓・骨髄axis調節の分子機構"臨床血液. 42. 397-402 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kato J, Kobune M, Kohgo Y, Fujikawa K, Takimoto R, Torimoto Y, Ito T, Bessho M, Hotta T, Hikawa A, Fujii T, Punnonen K, Niitsu Y: "Ratio of transferrin (Tf) to Tf-receptor complex in circulation differs depending on Tf iron saturation"Clin Chem 48 : . 2002. 48. 181-183 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ikuta K,Fujimoto Y,Suzuki Y,Tanaka K,Saito H,Ohhira M,Sasaki K,Kohgo Y.: "Overexpression of hemochromatosis protein, HFE, alters transferrin recycling process in human hepatoma cells."Biochim Biophys Acta. 1496・2-3. 221-231 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 鳥本悦宏,高後裕: "トランスフェリン受容体と赤血球造血"臨床血液. 41. 554-558 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Katsuya Ikuta, et al.: "Overexpression of hemochromatosis protein, HFE, alters transgerrin recycling process in human hepatoma cells"Biochim Biophys Acta. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Noriyasu Taya, et al.: "Fas-mediated apoptosis of peripheral blood mononuclear cells in patients with hepatitis C"Br J Haematol. (in press).

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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