| Project/Area Number |
11557072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Hematology
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| Research Institution | Tohoku University |
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Principal Investigator |
HATTORI Toshio Tohoku University, Internal Medicine, Professor, 大学院・医学系研究科, 教授 (30172935)
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| Co-Investigator(Kenkyū-buntansha) |
OHNO Isao Tohoku University, Hospital, Research Associate, 医学部・附属病院, 助手 (00250762)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2001: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2000: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1999: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | gene therapy / letivirus / vector / macrophages / infectious / レンチウィルス / 偽ウィルス / 平滑筋細胞 / レンチウィルスベクタ / VSV-G / 43Ti |
|---|
| Research Abstract |
Infectious diseases are a major cause of death in developing countries. Diseases such as ATL, AIDS and tuberculosis are still intractable in our country. In these disorders, macrophages play important roles for developing pathological changes. We have attempted to infect lentivirus vectors to infect human smooth muscle cells derived from the pulmonary artery. The lentivirus vectors are pseudotype viruses made of envelopes from X4, R5, X4R5 and VSV. The viruses could infect to corresponding target cells but could not infect to macrophages. To improve the infection to target cells, we have analyzed the conformational changes of gp41. These analyzes showed that human monoclonal antibody reacts with fusogenic structure of gp41, indicating that the structures are immunogenic. We also found that V3 loop peptide could enhance infection of the original viruses. Above studies would be applicable to the development of vectors for macrophages.
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