| Project/Area Number |
11557073
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TANI Kenzaburo IInst of Med Sci Univ of Tokyo, Associate Professor, 医科学研究所, 助教授 (00183864)
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| Co-Investigator(Kenkyū-buntansha) |
HAMADA Hiromi Tsukuba University Assistant Professor, 医学専門学群, 講師 (60261799)
TANIOKA Yoshikuni The Central for Experimental Animals, Chief of Animal Experimentation Center, 実験動物センター, センター長(研究職) (10072406)
ASANO Shigetaka Inst of Med Sci Univ of Tokyo, Professor, 医科学研究所, 教授 (50134614)
ISEKI Tohru IInst of Med Sci Univ of Tokyo, Research Associate, 医科学研究所, 助手 (10232365)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2000: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1999: ¥8,100,000 (Direct Cost: ¥8,100,000)
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| Keywords | common marmoset / human hematopoiesis model / leukemia model / xenograft / CD34 positive cell / cord blood cells / chimera hematopoiesis / gene transfer / 臍帯血細胞 / 胎児移植 / CD34 / フローサイトメトリー |
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| Research Abstract |
For the purpose of constructing preclinical monkey models with normal and leukemic human hematopoiesis, we have introduced small monkey of common marmoset. The followings are the progress in this year.
1) Construction of normal and leukemic human hematopoiesis in marmoset :
We transplanted CD34 positive human umbilical cord blood cells intraperitoneally to marmoset fetus under the guide of ultrasonograph. We have so far transplanted the CD34 positive human cord blood cells to 16 fetuses in 8 mother marmosets. We have also transplanted human leukemia cells of BV173, IMS-BC1 or Meg-01 to 14 fetuses in 7 mother marmosets. In the beginning abortions followed by the transplantations were experienced but the techniques have been improved using highly sensitive ultrasonography. Now we are following up carefully the in vivo constitution of human/marmoset chimera hematopoiesis in newborn marmosets.
2) Characterization of common marmoset lymphocytes : We have isolated marmoset lymphocyte fractions which were positive for human CD4, CD8 or CD56 monoclonal antibodies. The lymphocytes positive for human CD4 or CD8 antibody were considered also to be functional in marmosets. CD56 positive lymphocytes should furthermore be characterized to elucidate its characters.
We have so far demonstrated the similar characters of hematopoietic cells and lymphocytes between human and common marmosets. These findings support the possibility of constructing marmoset with human/marmoset hematopoietic chimera. Such marmosets would be very beneficial to study the safety and efficacy of newly developed gene transfer vectors as well as biological modifiers at preclinical levels. Although we have not successfully constructed such chimera marmosets yet, we have developed several new strategies which would make the xenografting more safely and efficiently. Further experiment is required to construct marmoset preclinical model system to study normal and leukemic human hematopoiesis.
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