| Project/Area Number |
11557103
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
HIDA Kazutoshi Hokkaido Univ., Grad. Schoolof Med., Lecturer, 医学部・附属病院, 講師 (10238305)
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| Co-Investigator(Kenkyū-buntansha) |
岩崎 喜信 北海道大学, 大学院・医学研究科, 教授 (00113522)
SEKI Toshitaka Hokkaido Univ. Hosp., Dept. Neurosurg., Research Fellow, 医学部・附属病院, 医員
TADA Mitsuhiro Hokkaido Univ., Institute for Genet. Med., Asso. Prof., 遺伝子病制御研究所, 助教授 (10241316)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2001: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2000: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1999: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | Spinal Cord / Injury / Apoptosis / Bcl-2 / Bax / Transgenic Mice / Knockout Mice |
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| Research Abstract |
In this research project, we investigated roles of neuronal cell apoptosis (programmed cell death) secondary to the primary injury in aggravation of traumatic lesion in the spinal cord. For this purpose, we used transgenic/knockout mice of Bcl-2 family genes which are important in regulation of cellular apoptosis. We first established a mouse model of spinal cord injury using a pneumatic impact device and its neurological evaluating system. Applying the model system on Bcl-2 transgenic mice, we obtained the following results:
1) Post traumatic neurologic recovery showed a trend to be better in Bcl-2 transgenic mice than in wild-type mice
2) Lesion volume assessed by histological quantative evaluation showed a statistically significant difference between wild-type mice (5.1 ± 0.8 x 10^<-2> ml) and Bcl-2 transgenic mice (2.9±0.5x10^<-2> ml).
These results indicate that extension of traumatic lesion after a spinal cord injury is largely affected by the-anti- and pro-apoptotic effects of Bcl-2 family proteins. We therefore suggest that Bcl-2 family proteins can be important therapeutic targets in prevention of secondary damage in spinal cord injury.
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