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Genetic alterations associated with malignancy of benign brain tumors.

Research Project

Project/Area Number 11557105
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field Cerebral neurosurgery
Research InstitutionYamaguchi University

Principal Investigator

NISHIZAKI Takafumi  Yamaguchi University, School of Medicine, Associate Professor, 医学部, 助教授 (10237688)

Co-Investigator(Kenkyū-buntansha) SASAKI Kohsuke  Yamaguchi University, School of Medicine, Professor, 医学部, 教授 (80116722)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordscytogenetics / CGH / LSC / SKY / meningioma / pituitary adenoma / schwannoma / genetic subgroups / spectral karyotyping / pilocyticastrocytoma / 悪性化
Research Abstract

Purpose : Little is known about genetic subgroups or genetic alterations during malignant progression in benign brain tumors. Since it is accepted that malignant tumors evolve by the accumulation of genetic aberrations during tumor progression, the investigation of genetic changes should provide some clues as to the mechanisms underlying malignant progression of benign brain tumors. In this study, we described the cytogenetic alterations detected by comparative genomic hybridization (CGH) analysis in benign brain tumors, and depicted a plausible genetic pathway for their progression. Methods : Using CGH and laser scanning cytometic (LSC) analyses, we investigated cytogenetic alterations and DNA ploidy patterns for meningiomas, pituitary adenomas, schwannomas and other brain tumors. In order to elucidate the relationship between chromosome structural disorders and DNA copy number aberrations in tumor cells, we applied the techniques of spectral karyotyping (SKY) for human glioma cell li … More nes. Results : The average number of alteration sites in each patient with typical meningioma was significantly less than those with atypical or anaplastic meningioma. The CGH findings suggest that losses of 1p, 2p, 6q, chromosome 10 and 14q, and gain of 20q are genetic changes implicated in the malignant progression of meningioma. The mean number of sites of copy gain was significantly higher in functioning adenomas than in non-functioning tumors. The DNA aneuploid adenomas showed cytogenetic changes more frequent than DNA diploid ones. Typical schwannomas displayed only a few genetic changes. Primary CNS lymphomas seem to have common genetic pathway with non-CNS ones. SKY, CGH and fluorescence in situ hybridization using yeast artificial chromosome probes for human glioma cell lines shed light on the close relationship between DNA amplification and chromosomal translocation. Conclusion : The data obtained from the further CGH analysis will allow a more definite genetic subgroups or progression pathways for benign brain tumors. Less

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Nishizaki T, et al: "Genetic alterations in pediatric medulloblastomas detected by comparative genomic hybridization."Pediatr Neurosurg. 31. 27-32 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 西崎隆文: "CGHによる脳腫瘍の細胞遺伝学的解析(ミニレビュー)"山口医学. 48. 195-203 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Harada K, et al.: "Cytogenetic alterations in pituitary adenomas detected by comparative genomic hybridization."Cancer Genet Cytogen. 112. 38-41 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ozaki S, et al.: "Comparative genomic hybridization analysis of genetic alterations associated with malignant progression of meningioma."Journal of Neuro-Oncology. 41. 167-174 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Harada K et al.: "Pediatric acoustic schwannoma showing rapid regrowth with high proliferative activity : Case report"Child's Nervous System. 16. 134-137 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kubota H, et al.: "Identification of recurrent chromosomal rearrangements and the unique relationship between low-level amplification and translocation in glioblastoma"Genes, Chromosomes Cancer. (In press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 小賀厚徳 他: "応用サイトメトリー,Comparative genomic hybridization(CGH)脳腫瘍"医学書院(天神美夫 監修). 4 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 小賀厚徳 他: "応用サイトメトリー,Comparative genomic hybridization(CGH)乳癌"医学書院(天神美夫 監修). 4 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takafumi Nishizaki, et al.: "Genetic alterations in pediatric medulloblastomas detected by comparative genomic hybridization."31. 27-32 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takafumi Nishizaki: "Journal Investigation of genetic alterations in brain tumors by CGH."Yamaguchi Medical. 48. 195-203 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsumi Harada, et al.: "Cytogenetic alterations in pituitary adenomas detected by comparative genomic hybridization."Cancer Genet Cytogen. 112. 38-41 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Satoshi Ozaki, et al.: "Comparative genomic hybridization analysis of genetic alterations associated with malignant progression. of meningioma"J Neuro-Oncol. 41. 167-174 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kei Harada, et al.: "Pediatric acoustic schwannoma showing rapid regrowth with high proliferative activity : Case report"Child's Nervous System. 16. 134-137 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hisashi Kubota, , et al.: "Identification of recurrent chromosomal rearrangements and the unique relationship between low-level amplification and translocation in glioblastoma."Genes, Chromosomes Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Atsunori Oga, et al.: "Comparative genomic hybridization (CGH). Brain tumor"Application of Cytometry. 303-306(4) (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Atsunori Oga, et al.: "Comparative genomic hybridization (CGH). Breast cancer"Application of Cytometry. 312-315(4) (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishizaki T, et al.: "Genetic alterations in pediatric medulloblastomas detected by comparative genomic hybridization."Pediatr Neurosurg. 31. 27-32 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 西崎隆文: "CGHによる脳腫瘍の細胞遺伝学的解析(ミニレビュー)"山口医学. 48. 195-203 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Harada K, Et al.: "Cytogenetic alterations in pituitary adenomas detected by comparative genomic hybridization."Cancer Genet Cytogen. 112. 38-41 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ozaki S, et al.: "Comparative genomic hybridization analysis of genetic alterations associated with malignant progression of meningioma."Journal of Neuro-Oncology. 41. 167-174 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Harada K et al.: "Pediatric acoustic schwannoma showing rapid regrowth with high proliferative activity : Case report"Child's Nervous System. 16. 134-137 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Harada K, et al.: "Cytogenetic Alterations in Primary Central Nervous System Lymphomas Detected by Comparative Genomic Hybridization and Laser Scanning Cytometry"Cancer Genet Cytogen. (In press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小賀厚徳: "応用サイトメトリー,Comparative genomic hybridization(CGH)脳腫瘍"医学書院(天神美夫監修). 4 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小賀厚徳: "応用サイトメトリー,Comparative genomic hybridization(CGH)乳癌"医学書院(天神美夫監修). 4 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Harada K, et al.: "Pediatric acoustic schwannoma showing rapid regrowth with high proliferative activity."Child's Nervous System. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 西崎隆文: "CGHによる脳腫瘍の細胞遺伝学的解析(ミニレビュー)"山口医学. 48. 195-203 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Harada K, et al.: "Cytogenetic alterations in pituitary adenomas detected by comparative genomic hybridization."Cancer Genet Cytogen. 112. 38-41 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ozaki S, et al.: "Comparative genomic hybridization analysis of genetic alterations associated with malignant progression of meningioma."J NeuroOncol. 41. 167-174 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kusano N, et al.: "Genetic aberrations detected by comparative genomic hybridization in hepatocellular carcinomas: their relationship to clinicopathological features."Hepatology. 29. 1858-1862 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miyazaki M, et al.: "The relationship of DNA ploidy to chromosomal instaility in primary human colorectal cancers."Cancer Res. 59. 5283-5285 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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