| Project/Area Number |
11557113
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B).
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
AOE Tomohiko Chiba University, Graduate School of Medicine, Associate Professor, 大学院・医学研究科, 助教授 (90311612)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KOSEKI Haruhiko Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (40225446)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2000: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1999: ¥6,800,000 (Direct Cost: ¥6,800,000)
|
|---|
| Keywords | stress protein / molecular chaperone / HSP27 / VP22 / heat shock protein / 酸化ストレス / 細胞保護 / 分泌 |
|---|
| Research Abstract |
Extracellular signalling is usually transduced to cells through binding of signalling molecules to the receptors on the cell surface or internalization of them by endocytosis machinery. Recently some proteins have been found to penetrate directly from extracellular space to cytoplasm and nucleus, and function there. VP22, a herpes simplex virus I protein, is such a protein that can penetrate to cytoplasm and nucleus. Interestingly, a chimeric protein with VP22 was also demonstrated to preserve such an ability. In this study, we made chimeric proteins with VP22 and HSP27, a chaperone that has a cytoprotective ability against cellular stresses, to explore a new drug delivery system. One chimeric VP22-HSP27 was an unstable protein that is degraded immediately after synthesis, the other VP22-HSP27 was more stable and we have detected its expression by immunofluorescence and western blot. The cells expressing this protein , unexpectedly, revealed sensitive to cellular stresses, which suggested the chimeric VP22-HSP27 had a dominant-negative character against endogenous HSP27. Further efforts making chimeric proteins with VP22 and HSP27, or other useful proteins will be explored.
|
