| Co-Investigator(Kenkyū-buntansha) |
MIYOSHI Norio Fukui Med.Univ., Assistant, 医学科, 助手 (40209961)
KOSHIDA Kiyoshi Kanazawa Univ., Lecturer, 医学部, 講師 (70186667)
UCHIBAYASHI Tadao Kanazawa Univ., Assoc.Prof., 医学部, 助教授 (90151894)
UDAGAWA Tsuyoshi IHI, Investigator, 開発部, 課長
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2000: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Research Abstract |
We investigated the photodynamic macrotic diagnosis (PDMD) of a cancer by a new semiconductor laser (iLS-LD400 model, 405 nm, 5 mW) using the photosensitizer protoporphyrin-IX (Pp-IX) generated in vivo from 5-aminolevulittic acid=5-ALA which has an affinity for the squamous cell carcinoma (SCC) tumor transplanted on the back of C3H/He mouse. In the result, it was found the specific affinity of the Pp-IX in the SCC tumor from the spectroscopic data that the Pp-IX fluorescent intensity in the tissue was enhanced 2 times by the ultrasound irradiation (47 kHz, 50 W, 10 min).
Furthermore, it was also investigated the combination therapy of photodynamic (PDT) and sonodynamic (SDT)(47 kHz, 50 W, 10 sec) treatments of a melanoma cultivated cell (HMF) using a sonosensitizer ATX-70, gallium porphyrin derivative, and the new laser iLS-LD400 (405 nm, 5 mW, 10 min). In the results, its was found that the necrotic cell death was enhanced by the combination therapy, especially in the case of the first treatment of the photoirradiation before the sonolysis, the enhancing effect was better than the other case.
In conclusion, it will be established to do the macrotic diagnosis the bladder tumor incorporated 5-ALA in the bladder model using a new semiconductor laser (iLS-LD400 model, IHI) and to treat the epithelium transitional cell carcinoma by the laser light.
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