• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study for preservation of dentin using local gene deliveryof dentin-specific genes

Research Project

Project/Area Number 11557143
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Conservative dentistry
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

TAKASHIBA Shogo  Okayama University Graduate School of Medicine and Dentistry, Department of Pathophysiology - Periodontal Science, Associate Professor, 大学院・医歯学総合研究科, 助教授 (50226768)

Co-Investigator(Kenkyū-buntansha) MYOKAI Fumio  Okayama University Graduate School of Medicine and Dentistry, Department of Pathophysiology - Periodontal Science, Instractor, 大学院・医歯学総合研究科, 助手 (50263588)
ARAI Hideo  Okayama University Graduate School of Medicine and Dentistry, Department of Pathophysiology - Periodontal Science, Assistant Professor, 歯学部・附属病院, 講師 (70222718)
MURAYAMA Yoji  Okayama University Graduate School of Medicine and Dentistry, Department of Pathophysiology - Periodontal Science, Professor, 大学院・医歯学総合研究科, 教授 (50029972)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥8,700,000 (Direct Cost: ¥8,700,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1999: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsDental pulp / Reparative dentin / Gene transcription / Local Gene Delivery / Tissue regeneration / 遺伝子サブトラクション
Research Abstract

Genes related to dentin regeneration were isolated by susbtractive hybridization from rat molar dental pulp after dentin cavity preparation. There were 16 induced and 7 reduced genes found after size selection (more than 250 bp). Induced genes included cytochrome c, cathepsin B, 3 EST genes (unknown function), and 1 novel gene. Reduced genes included ribosomal protein, laminin-γ2, type 1 collagen-α2, and 2 novels genes. Although in situ hybridization analysis of these genes was performed in order to elucidate their expression sites, no clear results have not been obtained. However, Northem blot analysis revealed that 1 EST gene showed most different expression signals compared before and after cavity preparation. Full length cDNA of this gene was cloned and nucleotide sequence analysis and homology search were performed. It is 3.8-kb length and has 83% homology to human FIP-2 (Adinovirus 14..7 KDa - interacting protein) in some part. Furthermore, this gene has longer putative coding region than that of FIP-2, and posess coild-coild domain including zinc finger domain and leuicin zipper domain. On the other hand, local gene delivery to dental pulp was performed using plasmid vector and andenovirus-associate vector using reporter gene. No significant difference of transfection efficiency was found between these vectors because cells on only the surface of dental pulp were transfected. Primitive problems of this study are 1) selection of genes for transfection and 2) efficiency of local gene delivery. The latter problem would be solved by modification or development of vectors for high transfection efficiency and repeated use. The former problem would be solved by identification of genes as this study. However, additional problem will arise where each gene should be transfected.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Oyama M, 他: "Novel genes expressed in injured dental pulp"Journal of Dental Research. 80巻・(Special Issue). 796-796 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sonoyama, W, 他: "Immunohistochemical localization of connective tissue growth factor during reparative dentinogenesis"Journal of Dental Research. 80巻・(Special Issue). 59-59 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sonoyama, W, 他: "Effects of proinflammatory factors on CTGF expression in odontoblast-like cells"Journal of Dental Research. 81巻・(Special Issue). A-155-A-155 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oyama M, 他: "Isolation of genes expressed in rat injured dental pulp"Journal of Dental Research. 79巻・(Special Issue). 176-176 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oyama M, et al: "Isolation of genes expressed in rat injured dental pulp."Journal of Dental Research. 79(Special Issue). 176 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oyama M, et al: "Novel genes expressed in injured dental pulp."Journal of Dental Research. 80(Special Issue). 796 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sonoyama, W, et al: "Immunohistochemical localization of connective tissue growth factor during reparative dentinogenesis."Journal of Dental Research. 80(Special Issue). 59 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sonoyama, W, et al: "Effects of proinflammatory factors on CTGF expression in odontoblast-like cells."Journal of Dental Research. 81(Special Issue A). A-155 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oyama M, 他: "Novel genes expressed in injured dental pulp"Journal of Dental Research. 80巻・(Special Issue). 796-796 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sonoyama, W, 他: "Immunohistochemical localization of connective tissue growth factor during reparatibe dentinogenesis"Journal of Dental Research. 80巻・(Special Issue). 59-59 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sonoyama W, 他: "Effects of proinflammatory factors on CTGF expression in odontoblast-like cells"Journal of Dental Research. 81巻・(Special Issue)(予定). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Oyama M, 他: "Isolation of Genes Expressed in Rat Injured Dental Pulp"Journal of Dental Reserch. 79卷(Special Issue). 176-176 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Oyama M, 他: "Novel Genes Expressed in Injured Dental Pulp"Journal of Dental Reserch. 80卷(Special Issue)(予定). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M. Oyama et al.: "Isolation of genes expressed in rat injured dental pulp"Journal of Dental Research. 79(Special Issue)(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 高柴正悟: "歯髄疾患の診断 新しい治療へのブレークスルー 歯髄保存をめざす歯髄疾患診断の将来"日本歯科保存学雑誌. 42(秋期特別号). 17-17 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 高柴正悟: "歯髄組織の創傷治癒時に特徴的に発現する遺伝子"日本歯科保存学雑誌. 42(秋期特別号). 17-17 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 尾山正高: "歯髄細胞への外来遺伝子の導入に関する実験的研究"日本歯科保存学雑誌. 42(秋期特別号). 18-18 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi