| Project/Area Number |
11557159
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Surgical dentistry
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
IIZUKA Tadahiko Kyoto Univ., Faculty of Medicine, Professor, 医学研究科, 教授 (80026921)
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| Co-Investigator(Kenkyū-buntansha) |
KUSUMOTO Takashi Kyoto Univ., Faculty of Medicine, Assistant Professor, 医学研究科, 助手 (10301251)
YOKOE Yoshihiko Kyoto Univ., Faculty of Medicine, Assistant Professor, 医学研究科, 助手 (30211650)
WAKATSUKI Yoshio Kyoto Univ., Faculty of Medicine, Assistant Professor, 医学研究科, 講師 (40220826)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Helicobacter pylori / oral cancer / oral lichen planus / Pradication / Helicobacter pylori / oral lichen planus / PCR / CagA / VacA / 唾液 / 経口免疫 / マウス / Peyer's Patch |
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| Research Abstract |
Considering a suspected link between Helicobacter pylori infection and human oral cancer, we had cloned a new H.pylori gene for membrane protein 1 (HP-MP1) at first. Because HP-MP1 induces release of inflammatory cytokines and tumor necrosis factor-alpha acts as both initiator and tumor promoter, we studied the possible involvment of HP-MP1 in carcinogenesis of H.pylori. Two cell lines, BALB/3T3 cells as control and v-Ha-ras-transfected BALB/3T3 cells as putative initiated cells, were each transfected with HP-MP1, urease B genes, or vector alone. All of the Bhas/mpl clones showed strong expression of tumor necrosis factor-alpha gene and produced tumors in 100 % of nude mice. Two Bhas/ure clones showed weak tumorigenicity ; the other Bhas and BALB clones showed none. Results indicate strong carcinogenic activity of HP-MP1 in cooperation with viral Ras protein and weak activity of urease B.
Secondly, we often see the changes Oral lichen planus(OLP) for cancer. We found that 50 % of OLP patients infected with H.pylori. So, we analyzed the effect the eradication therapy for H.pylori. 5 OLP patients who had H.pylori infection treated with the eradication therapy. 3 of 5 patients were effective for 3 months, but 2 of 3 patients complaned pain 6 months later. Only one patient was effective for 12months. 2 of 5 patients were not effective at all. Thus, it is considerd that there is ho relationship between H.pylori infection and OLP.
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