Establishment of a study model for the recovery of oral functions in patients suffering from somatosensory disfunction
| Project/Area Number |
11557164
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
SHIRAKAWA Tetsuo Hokkaido Univ., Dent.Hospital, Lec., 歯学部・附属病院, 講師 (00187527)
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| Co-Investigator(Kenkyū-buntansha) |
HONMA Sato Hokkaido Univ., Grad.School of Med., Asso.Prof., 大学院・医学研究科, 助教授 (20142713)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2000: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥9,900,000 (Direct Cost: ¥9,900,000)
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| Keywords | Multi-electrode dish / Circadian rhythm / Suprachiasmatic nucleus / Neural network / Nucleus of the solitary tract / Reticular formation / Hypoglossal nucleus / Trigeminal nucleus / 分散培養 / 静置器官培養 / 神経幹細胞 |
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| Research Abstract |
The present study was carried out to establish a study model for the recovery of oral functions in patients suffering from somatosensory disfunction.
We used dissociated cell culture of rat suprachiasmatic nucleus (SCN) neurons because the function of the SCN as a circadian pacemaker has been investigated enthusiastically in both in vivo and in vitro. By using a multi-electrode dish, we measured circadian firing rhythms in individual SCN neurons for more than 2 weeks continuously. Cross-correlation analysis of spontaneous action potentials revealed that a neuron pair was functionally connected by synapses when their circadian rhythms were synchronized. No correlation was found between the paired neurons whose circadian rhythms were not synchronized. Desynchronization of the firing rhythms and the disappearance of the cross-correlation after a chronic blockade of neural communication by tetrodotoxin demonstrate a causal relationship between synaptic transmission and rhythm synchrony.
Feeding-associated activation of neurons in the medulla oblongata was examined in both developing and adult rats, using the c-fos gene-encoded protein (Fos) as a marker. Neonates were isolated from mothers for 12h on postnatal day 3,7 and 14, and either breast-fed intensively or further isolated for another 3h, and sacrificed. Adult rats were fasted for 24 hours and either given food and water before sacrifice or sacrificed without food intake. In the breast-fed pups, Fos-immunoreactive (FI) neurons were predominantly localized in the nucleus of the solitary tract (NST) and dorsal medullary reticular formation (dRF). Satiated adult rats with food showed similar patterns of Fos expression in both NST and dRF.In contrast, much fewer FI cells were observed in the NST and dRF in the fasted pups and adults. The distinct localization of Fos in fed pups and adults indicates functional significance of the NST and dRF in the feeding behavior.
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Report
(3 results)
Research Products
(18 results)
