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Establishment of a study model for the recovery of oral functions in patients suffering from somatosensory disfunction

Research Project

Project/Area Number 11557164
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field 矯正・小児・社会系歯学
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

SHIRAKAWA Tetsuo  Hokkaido Univ., Dent.Hospital, Lec., 歯学部・附属病院, 講師 (00187527)

Co-Investigator(Kenkyū-buntansha) HONMA Sato  Hokkaido Univ., Grad.School of Med., Asso.Prof., 大学院・医学研究科, 助教授 (20142713)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2000: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥9,900,000 (Direct Cost: ¥9,900,000)
KeywordsMulti-electrode dish / Circadian rhythm / Suprachiasmatic nucleus / Neural network / Nucleus of the solitary tract / Reticular formation / Hypoglossal nucleus / Trigeminal nucleus / 分散培養 / 静置器官培養 / 神経幹細胞
Research Abstract

The present study was carried out to establish a study model for the recovery of oral functions in patients suffering from somatosensory disfunction.
We used dissociated cell culture of rat suprachiasmatic nucleus (SCN) neurons because the function of the SCN as a circadian pacemaker has been investigated enthusiastically in both in vivo and in vitro. By using a multi-electrode dish, we measured circadian firing rhythms in individual SCN neurons for more than 2 weeks continuously. Cross-correlation analysis of spontaneous action potentials revealed that a neuron pair was functionally connected by synapses when their circadian rhythms were synchronized. No correlation was found between the paired neurons whose circadian rhythms were not synchronized. Desynchronization of the firing rhythms and the disappearance of the cross-correlation after a chronic blockade of neural communication by tetrodotoxin demonstrate a causal relationship between synaptic transmission and rhythm synchrony.
Feeding-associated activation of neurons in the medulla oblongata was examined in both developing and adult rats, using the c-fos gene-encoded protein (Fos) as a marker. Neonates were isolated from mothers for 12h on postnatal day 3,7 and 14, and either breast-fed intensively or further isolated for another 3h, and sacrificed. Adult rats were fasted for 24 hours and either given food and water before sacrifice or sacrificed without food intake. In the breast-fed pups, Fos-immunoreactive (FI) neurons were predominantly localized in the nucleus of the solitary tract (NST) and dorsal medullary reticular formation (dRF). Satiated adult rats with food showed similar patterns of Fos expression in both NST and dRF.In contrast, much fewer FI cells were observed in the NST and dRF in the fasted pups and adults. The distinct localization of Fos in fed pups and adults indicates functional significance of the NST and dRF in the feeding behavior.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report

Research Products

(18 results)

All Other

All Publications

  • [Publications] Shirakawa,T.: "Multiple oscillators in the suprachiasmatic nucleus."Chronobiology Int.. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mitome,M.: "Circadian rhythm of nitric oxide production in the dorsal region of the suprachiasmatic nucleus in rats."Neurosci.Lett.. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shirakawa,T.: "Synchronization of circadian firing rhythms in cultured rat suprachiasmatic neurons."Eur.J.Neurosci.. 12. 2833-2838 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Honma,S.: "Synaptic communication of cellular oscillations in the rat suprachiasmatic neurons."Neurosci.Lett.. 294. 113-116 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hironaka,S.: "Feeding-induced c-fos expression in the nucleus of the solitary tract and dorsal medullary reticular formation in neonatal rats."Neurosci.Lett.. 293. 175-178 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Honma,S.: "Multiple circadian oscillators in the SCN and multiple pacemakers outside the SCN. (In : K.Honma & S.Honma, eds.Zeigebers, Entrainment and Masking of the Circadian System.)"Hokkaido Univ.Press, Sapporo. 299 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shirakawa T, Honma S, Honma K: "Multiple oscillators in the suprachiasmatic nucleus."Chronobiology Int. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mitome M, Shirakawa T, Oshima S, Nakamura W and Oguchi H: "Circadian rhythm of nitric oxide production in the dorsal region of the suprachiasmatic nucleus in rats."Neurosci Lett. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shirakawa T, Honma S, Katsuno Y, Oguchi H, Honma K: "Synchronization of circadian firing rhythms in cultured rat suprachiasmatic neurons."Eur J Neurosci. 12. 2833-2838 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hironaka S, Shirakawa T, Toki S, Kinoshita K, Oguchi H: "Feeding-induced c-fos expression in the nucleus of the solitary tract and dorsal medullary reticular formation in neonatal rats."Neurosci Lett. 293. 175-178 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Honma S, Shirakawa T, Nakamura W and Honma K: "Synaptic communication of cellular oscillations in the rat suprachiasmatic neurons."Neurosci Lett. 294. 113-116 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Honma S, Masubuchi S, Nakamura W, Shirakawa T and Honma K: "Multiple circadian oscillators in the SCN and multiple pacemakers outside the SCN."In Zeitgebers, Entrainment and Masking of the Circadian System, ed.by K.Honma and S.Honma. Hokkaido Univ. Press, Sapporo. 239-250 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shirakawa, T.: "Multiple oscillators in the suprachiasmatic nucleus."Chronobiology Int.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mitome, M.: "Circadian rhythm of nitric oxide production in the dorsal region of the suprachiasmatic nucleus in rats."Neurosci.Lett.. in press- (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shirakawa, T.: "Synchronization of circadian firing rhythms in cultured rat suprachiasmatic neurons."Eur.J.Neurosci.. 12. 2833-2838 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Honma, S.: "Synaptic communication of cellular oscillations in the rat suprachiasmatic neurons."Neurosci.Lett.. 294. 113-116 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hironaka, S.: "Feeding-induced c-fos expression in the nucleus of the solitary tract and dorsal medullary reticular formation in neonatal rats."Neurosci.Lett.. 293. 175-178 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Honma, S.: "Multiple circadian oscillators in the SCN and multiple pacemakers outside the SCN. (In : K.Honma & S.Honma, eds.Zeigebers, Entrainment and Masking of the Circadian System.)"Hokkaido Univ.Press, Sapporo (in press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 1999-03-31   Modified: 2016-04-21  

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