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The method for determination of serum lipid-free apolipoprotein concentration and its clinical application

Research Project

Project/Area Number 11557204
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Laboratory medicine
Research InstitutionGifu University

Principal Investigator

SEISHIMA Mitsuru  Gifu University, Department of Laboratory Medicine, Professor, 医学部, 教授 (10171315)

Co-Investigator(Kenkyū-buntansha) WADA Hisayasu  Gifu University, Department of laboratory Medicine, Lecturer, 医学部, 助手 (10283300)
SAITO Kuniaki  Gifu University, Department of Laboratory Medicine, Assistant Professor, 医学部・附属病院, 講師 (80262765)
MINATOGUCHI Shinya  Gifu University, 2nd Department of Internal Medicine, Associate Professor, 医学部, 助教授 (20190697)
SAITO Kazunori  Daiichi Pure Chemicals Company, Research Manager, 診断薬研究所, リサーチマネージャー
斎藤 和典  第一化学(株), 診断薬研究所, リサーチマネージャー
藤井 秀比古  岐阜大学, 医学部, 助手 (50301213)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1999: ¥6,800,000 (Direct Cost: ¥6,800,000)
KeywordsLipid-free-free apolipoprotein / Corobary heart disease / Atherosclerosis / pre βHDL / Coronary heart disease / ELISA / preβ HDL
Research Abstract

It is known that HDL are heterogeneous in particle size and small HDL particle can promote more effectively cholesterol efflux from cell membrane than large HDL particle. Small HDL are detected by two-dimentional gel electrophoresis, but this method is time consuming. Lipid-free apo A-I in this study was separated by HPLC and was elutred from 51 min to 56 min. The epitope of lipid-free apo A-I is the same as tjhat of a HDL, because we used the antibody to apo A-I in the ELISA system. We added SOU/ml of trasyrol to blood and also to 500 μ1 of fractionated solutions by HPLC. Serum lipid-free apo A-I concentration increased two times by repeated freeze and thawing test and changed with time even in the store at 4℃. Thus, serum sample was immediately treated for HPLC or freezed at -80℃ until use.
Serum lipid-free apo A-I concentrations significantly increased in patients with coronary heart disease compared to healthy controls in both males and females. These findings show that reverse cholesterol transport is altered in coronary heat disease and the measurement of lipid-free apo A-I may be a good marker for atherosclerosis.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Fei H, et al.: "Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum"Clin. Chem..

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wada H, et al.: "Tumor necrosis factor-α (TNF-Q) plays a protective role in acute viral myocarditis in mice : A study using mice lacking TNF-α"Circulation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Umeda Y, et al.: "Inhibitory action of gemfibrozil on cholesterol absorption in rat intestine"J. Lipid Res..

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujii H, et al.: "Immunohistochemical localization and mRNA expression of apolipoprotein A-I in rat spinal cord"J. arterioscler. Thromb. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 清島 満, 他: "高脂血症(上巻)リポ蛋白X"日本臨床.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 清島 満, 他: "脂質検査について"臨床病理.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 藤井秀比古 他: "医学のあゆみ「サイトカインと疾患」"医歯薬出版.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fei,H., etal.: "Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum"Clin Chem. 46. 1351-1356 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wada,H., et al.: "Tumor necrosis factor-α ( TNF-α ) plays a protective role in acute viral myocarditis in mice: A study using mice lacking TNF-α"circulation. 103. 743-749 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Umeda,Y., et al.: "Inhibitory action of gemmfibrozil on cholesterol absorption in rat intestine"J Lipid Res. 42. 1214-1219 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Seishima,M., et al.: "Reference values of serum lipid"Rinsho Byori. 49. 1115-1121 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maekawa,N.,: "Improved myocardial ischemia/reperfusion injury in mice lacking tumor necrosis factor-α"J am Coll Cardiol. 39 in press. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujii,H., et al.: "Immunohistochemical localization and mRNA expression of apolipoprotein A-I in rat spinal cord"J Atheroscler Thromb. 9 ( in press ). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fei H, et al.: "Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum"Clin. Chem.. 46・9. 1351-1356 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Wada H, et al.: "Tumor necrosis factor-α(TNF-α) plays a protective role in acute viral myocarditis in mice : A study using mice lacking TNF-α"Circulation. 103・5. 626-629 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Umeda Y, et al.: "Inhibitory action of gemfibrozil on cholesterol absorption in rat intestine"J. Lipid Res.. 42・8. 1214-1219 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fujii H, et al.: "Immunohistochemical localization and mRNA expression of apolipoprotein A-I in rat spinal cord"J. arterioscler. Thromb.. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 清島 満 他: "高脂血症(上巻) リポ蛋白X"日本臨床. 59・suppl. 91-95 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 清島 満 他: "脂質検査について"臨床病理. 49・11. 1115-1121 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 藤井秀比古 他: "医学のあゆみ「サイトカインと疾患」"医歯薬出版. 222 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 清島満: "Total cholesterol(T-CHO)の基準値について"クリニシアン. 46. 691-693 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 清島満: "リポ蛋白と動脈硬化"岐阜県医師会医学雑誌. 12. 3-9 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 清島満 他: "高脂血症の診断プロトコル"Medical Technology. 27. 398-406 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fei H, et al.: "Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum"Clin.Chem.. 46. 1351-1356 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Wada H, et al.: "Tumor necrosis factor-α (TNF-α) plays a protective role in acute viral myocarditis in mice : A study using mice lacking TNF-α"Circulation. 103. 743-749 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 藤井秀比古 他: "医学のあゆみ「サイトカインと疾患」"医歯薬出版. 4

    • Related Report
      2000 Annual Research Report
  • [Publications] Vilpo J,et al.: "Blood soluble Fas/Apo-1 (CD25) in chronic lymphocytic leukemia"Eur.J.Haematol.. 62. 352-353 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 清島 満 他: "リポ蛋白X(X)"日本臨床. 57. 39-41 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 清島 満 他: "リポ蛋白(a)とそのフェノタイプ"日本臨床. 57. 42-44 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 浦上克哉 他: "髄液中アポA-IおよびE"日本臨床. 57. 158-161 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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