| Project/Area Number |
11558073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
環境保全
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
HARADA Hideki Nagaoka University of Technology, Faculty of Engineering, Professor, 工学部, 教授 (70134971)
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| Co-Investigator(Kenkyū-buntansha) |
OHASHI Akiyoshi Nagaoka University of Technology, Faculty of Engineering, Assoc.Professor, 工学部, 助教授 (70169035)
SEKIGUCHI Yoji Nagaoka University of Technology, Faculty of Engineering, Assis.Professor, 工学部, 助手 (20313570)
ARAKI Nobuo Nagaoka University of Technology, Faculty of Engineering, Assoc.Professor, 環境都市工学科, 助教授 (30193072)
TANAKA Toshihiro EBARA Corporation, Environ.Eng.Group, General Manager, 環境開発センター, 応用技術部長
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2000: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1999: ¥8,800,000 (Direct Cost: ¥8,800,000)
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| Keywords | UASB process / Thermophilic methane fermentation / Anaerobic wastewater treatment / Granular sludge / Methaiiogen / Pilotscale / FISH / 16S rRNA |
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| Research Abstract |
Whole cell in-situ hybridization with 16S rRNA targeted oligonucleotides was used to clarify the microbial ecological structure of sludge granules developed in laboratory- and pilot-scale upflow anaerobic sludge blanket (UASB) reactors receiving various types of wastewaters. Three different molecular probes : ARC915, complementary to conserve region of the 16S rRNA of the domain Archaea ; MT757, for genus Methanosaeta (Methanothrix), and MB1174, for Methanobacteriales, were employed in this study. The FISH results indicated that genera Archaea, Methanosaeta and Methanobacteriales accounted for approximately 30 to 60 %, 30 to 55%, and 10 to 30%, respectively of the DAPI (6 diamidino-2-phenylindole) stained cells (with responding to the total cell number) in the respective granular sludges.
A strong correlation was observed between cell densities and methanogenic activities for both the Methanosaeta (acetate fed) and Methanobacteriales (hydrogen fed) genera. Topology of microbial structure of both the mesophilically- and thermophilically grown granules was investigated by dividing the individual whole granules into the outer-layer and the inner-layer portions. The methanogens were found more significant in the inner-layer portion. Similarly, thermophilic granules contained higher percentage of Methanobacter group that utilized hydrogen or formate for methanogenesis, as compared to the mesophilic. This suggests that syntrophic acetate oxidizing methanogenesis through hydrogen formation becomes more prominent under thermophilic conditions.
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