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Development of analytical technique of change in microbial population for remediation in the polluted soil

Research Project

Project/Area Number 11558075
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 環境保全
Research InstitutionToyohashi University of Technology

Principal Investigator

FUJIE Koichi  Toyohashi University of Technology, Department of Ecological Engineering, Professor, 工学部, 教授 (30134836)

Co-Investigator(Kenkyū-buntansha) DAIMON Hiroyuki  Toyohashi University of Technology, Department of Ecological Engineering, Assistant Professor, 工学部, 助手 (60335106)
GOTO Naohiro  Toyohashi University of Technology, Department of Ecological Engineering, Associate Professor, 工学部, 助教授 (50303706)
HIRAISHI Akira  Toyohashi University of Technology, Department of Ecological Engineering, Professor, 工学部, 教授 (40283486)
胡 洪営 (胡 供営)  豊橋技術科学大学, 工学部, 助教授 (30262976)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 2001: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥6,200,000 (Direct Cost: ¥6,200,000)
KeywordsMicrobial community structure / Quinone profile / FISH / CTC reduction / Soil microorganism / Tidal flat ecosystem / Remediation / Bacteria extraction / DTAF / CTC / 土壤汚染 / FISH / キノン量 / PCR / 土壌汚染 / バイオマス / 微生物多様性
Research Abstract

Microorganisms perform an important role for remediation in various polluted environment. To optimize the activity of microorganisms on contaminated sites, the ability of microorganisms must be clarified on the optimal condition for decomposing bacteria. The behavior of specific bacteria and microbial population should be clarified. The objective of this study is to develop the analytical technique for clarification of the behavior of specific bacteria and microbial community structure in natural waters and soils.
The direct application of molecular genetic methods on soil bacteria is often prevented by the large amounts of soil colloids. Hence, an extraction of bacteria cells from soil is usually required before analysis of their genetic material. For this reason, the selection of a suitable extractant was investigated. The extraction efficiency of bacteria and the representation of the microbial population extracted from soil by using quinone-profiling method were evaluated in the exp … More eriments. The fraction of the respiratory active cell extracted from soil was also evaluated by using CTC (5-cyano-2, 3-ditolyl tetorazolium chloride) reduction and plate count techniques. 1/15M Na-phosphate, 0.03M Na-oxalate and 0.03M Na-pyrophosphate were used as the extractants. The extraction efficiency of bacteria was defined as the ratio of quinone contents in the extract to that in soil. Compared with other two extractants, Na-pyrophosphate demonstrated the highest extraction efficiency of bacteria and respiratory active cells in the extract from soil. In addition, cell numbers of Proteobacteria, and γ-subclass in extraction with Na- pyrophosphate was measured by FISH. Almost all bacteria of Proteobacteria α, β and γ-subclass contain UQ-10, UQ-8, UQ-9, respectively. The experimental results showed that there was very similar relationship between ratio of Proteobacteria β, γ to α-subclasses and ratio of UQ-8, UQ-9 to UQ-10, respectively. It indicates that specific bacteria in soil sample can be quantified by FISH technique.
In addition, the quinone profile and CTC reduction can be used to clarify the effect of particle size of sediment on microbial-ecosystem and water purification using an experimental facility of the tidal flat, and to analyze microbial community structure in sediment. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (52 results)

All Other

All Publications (52 results)

  • [Publications] Hong-Ying Hu: "Quantitative analyses of the change in microbial diversity in a bioreactor for wastewater treatment based on respiratory quinones"Water Research. 33(15). 3263-3270 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hong-Ying Hu: "Development of a novel solid phase extraction method for the analysis of bacterial quinones in activated sludge with a higher reliability"Journal of Bioscience and Bloengineering. 87(3). 378-382 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuaki Saitou: "Detection of microbial groups metabolizing a substrate in soil based on the[^<14>C]quinone profile"Soil Science and Plant Nutrition. 45(3). 669-679 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuaki Saitou: "Linear relation between the amount of respiratory quinones and the microbial biomass in soil"Soil Science and Plant Nutrition. 45(3). 775-778 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Satoko Uchida: "Changes in microbial community structure in soil percolated with pentachlorophenol with reference to respiratory quinone profile"Journal of Pesticide Science. 24. 186-188 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hong-Ying Hu: "Characterization of microbial community in an activated sludge process treating domestic wastewater using quinone profiles"Water Science and Technology. 43(1). 99-106 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akira Hiraishi: "Toward the bioremediation of dioxin-polluted soil : structural and functional analyses of in situ microbial populations by quinone profiling and culture-dependent methods"Applied Microbiology and Biotechnology. 57. 248-256 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Arata Katayama: "Changes in the respiratory quinone profile of a soil treated with pesticides"Biology and Fertility of Soils. 33. 454-459 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hong-Ying Hu: "Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples"Journal of Microbiological Methods. 47(1). 17-24 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 内藤ゆかり: "キノンプロファイル法による池微生物群集構造と水質との関係解析"第33回日本水環境学会講演集. 220 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 林炳蘭: "キノンプロファイル法による生活排水処理活性汚泥曝気槽内微生物群集の季節変動の解析"第33回日本水環境学会講演集. 245 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 國弘忠生: "キノンプロファイル法による河川微生物相変化の解析"環境科学会1999年年会講演集. 92-93 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 國弘忠生: "キノンによる土壌微生物抽出法の評価"日本水環境学会シンポジウム. 73 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 國弘忠生: "土壌より抽出された微生物の群集構造の評価"日本微生物生態学会講演集. 138 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 平石明: "Dioxin汚染土壌の微生物調査と生物学的環境修復の可能性についての考察"日本農芸化学会講演集. 388 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 國弘忠生: "土壌微生物定量化方法の検討"第35回水環境学会年会講演要旨集. 535 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 佐久間元成: "干潟における微生物群集構造の解析"第35回水環境学会年会講演要旨集. 256 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 佐久間元成: "干潟における底質特性と微生物群集構造の関係解析"第36回水環境学会年会講演要旨集. 125 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hong-Ying Hu, Koichi Fujie, Hiroki Nakagome, Kohei Urano and Arata Katayama: "Quantitative analyses of the change in microbial diversity in a bioreactor for wastewater treatment based on respiratory quinones"Water Research. 33(15). 3263-3270 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hong-Ying Hu, Koichi Fujie and Kohei Urano: "Development of a novel solid phase extraction method for the analysis of bacterial quinones in activated sludge with a higher reliability"Journal of Bioscience and Bioengineering. 87(3). 378-382 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuaki Saitou, Koichi Fujie and Arata Katayama: "Detection of microbial groups metabolizing a substrate in soil based on the [^<14>C] quinone profile"Soil Science and Plant Nutrition. 45(3). 669-679 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuaki Saitou, Ken-ichiro Nagasaki, Haruyoshi Yamanaka, Hong-Ying Hu, Koichi Fujie and Arata Katayama: "Linear relation between the amount of respiratory quinones and the microbial biomass in soil"Soil Science and Plant Nutrition. 45(3). 775-778 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Satoko Uchida, Hajime Tanaka, Koichi Fujie and Arata Katayama: "Changes in microbial community structure in soil percolated with pentachlorophenol with reference to respiratory quinone profile"Journal of Pesticide Science. 24. 186-188 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hong-Ying Hu, Byung-Ran Lim, Naohiro Goto, Vishvesh K. Bhupathiraju and Koichi Fujie: "Characterization of microbial community in aan activated sludge process treating domestic wastewater using quinone profiles"Water Science and Technology. 43(1). 99-106 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akira Hiraishi, Hideki Miyakoda, Byung-Ran Lim, Hong-Ying Hu, Koichi Fujie and Jiro Suzuki: "Toward the bioremediation of dioxin-polluted soil: structural and functional analyses of in situ microbial populations by quinone profiling and culture-dependent methods"Applied Microbiology and Biotechnology. 57. 248-256 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Arata Katayama, Keiko Funasaka and Koichi Fujie: "Changes in the respiratory quinone profile of a soil treated with pesticides"Biology and Fertility of Soils. 33. 454-459 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hong-Ying Hu, Byung-Ran Lim, Naohiro Goto and Koichi Fujie: "Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples"Journal of Microbiological Methods. 47(1). 17-24 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tadao Kunihiro, Byung-Ran Lim, Yukari Naito, Hong-Ying Hu, Naohiro Goto and Koichi Fujie: "Analysis of Microbial Community Change in River Using Quinone Profile"Proceedings of 1999 Annual Conference of Janan Society of Environmental Science. 92-93 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yukari Naito, Byung-Ran Lim, Tadao Kinihiro, Hong-Ying Hu, Naohiro Goto and Koichi Fujie: "Analyses of Water Quality and Microbial Community Change in Ponds Using Quinone Profile"Proceedings of 33^<rd> Annual Conference of Japan Society on Water Environment. 220 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Byung-Ran Lim, Hong-Ying Hu, Naohiro Goto and Koichi Fujie: "Analysis of the Seasonal Change in Microbial Phase in an Aeration Tank Treating Domestic Water using Quinone Profile"Proceedings of 33^<rd> Annual Conference of Japan Society on Water Environment. 245 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tadao Kunihiro, Hong-Ying Hu, Naohiro Goto and Koichi Fujie: "Investigation of Quantitative Technique for Microorganisms in Soil"Proceedings of 35^<th> Annual Conference of Japan Society on Water Environment. 535 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tadao Kunihiro, Hong-Ying Hu, Naohiro Goto and Koichi Fujie: "Evalution of Direct Extraction Method for Bacteria in Soil Based on Quinone Profile"Proceedings of Symposium of Japan Society on Water Environment. 73 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Motonari Sakuma, Tadao Kunihiro, Naohiro Goto, Koichi Fujie, Teruaki Suzuki and Hiroaki Aoyama: "Analysis of Microbiai Community Structure in Tidal flat"Proceedings of 35^<th> Annual Conference of Japan Society on Water Environment. 256 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Motonari Sakuma, Tadao Kunihiro, Masafumi Fujita, Naohiro Goto, Koichi Fujie, Teruaki Suzuki and Hiroaki Aoyama: "Analysis of Relationship between Soil Properties and Microbial Community Structure in Tidal Flat"Proceedings of 36^<th> Annual Conference of Japan Society on Water Environment. 125 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A.HIRAISHI, H.MIYAKODA, B.-R.LIM, H.-Y.HU, K.FUJIE, J.SUZUKI: "Toward the bioremediation of dioxin-polluted soil:structural and functional analyses of in situ microbial populations by quinone profiling and culture-dependent methods"Appl.Microbiol Biotechnol.. 57. 248-256 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Katayama, K.Funasaka, K.Fujie: "Changes in the respiratory quinone profile of a soil treated with pesticides"Biol.Fertil.Soils. 33. 454-459 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.-Y.HU, B.-R.Lim, N.Goto, K.Fujie: "Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples"Journal of Microbiological Methods. 47(1). 17-24 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 國弘忠生, 胡 洪営, 後藤尚弘, 藤江幸一: "土壌微生物定量化方法の検討"第35回水環境学会年会講演要旨集. 535 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐久間元成, 國弘忠生, 胡洪営, 後藤尚弘, 藤江幸一, 鈴木輝明, 青山裕晃: "干潟における微生物群集構造の解析"第35回水環境学会年会講演要旨集. 256 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐久間元成, 國弘忠生, 藤田昌史, 後藤尚弘, 藤江幸一, 胡洪営、他: "干潟における底質特性と微生物群集構造の関係解析"第36回水環境学会年会講演要旨集. (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hong-Ying Hu: "Quantitative Analyses of the Change in Microbial Diversity in a Bioreactor for Wastewater Treatment based on Respiratory Quinones"Water Research. 33(15). 3263-3270 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hong-Ying Hu: "Development of a Novel Solid Phase Extraction Method for the Analysis of Bacterial Quinones in Activated Sludge with a Higher Reliability"J.Bioscience and Bioeng.. 87(3). 378-382 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Katsuaki Saitou: "Linear relation between the amount of respiratory quinones and the microbial biomass in soil"Science and Plant Nutrition. 45(3). 775-778 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Satoko Uchida: "Changes in microbial community structure in soil percolated with pentachlorophenol with reference to respiratory quinone profile"J.Pesticide Sci.. 24(2). 186-188 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 国弘忠生: "土壌より抽出された微生物の群集構造の評価"日本微生物生態学会(稲敷). 138 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hong-Ying Hu: "Characterization of microbial community in an activated sludge process treating domestic wastewater using quinone profiles"Water Science and Technology. 43(1). 99-106 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hong-Ying Hu: "Quantitative Analyses of the Change in Microbial Diversity in a Bioreactor for Wastewater Treatment based on Respiratory Quinones"Water Research. 33(15). 3263-3270 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hong-Ying Hu: "Development of a Novel Solid Phase Extraction Method for the Analysis of Bacterial Quinones in Activated Sludge with a Higher Reliability"J.Bioscience and Bioeng.. 87(3). 378-382 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuaki Saitou: "Linear relation between the amount of respiratory quinones and the microbial biomass in soil"Science and Plant Nutrition. 45(3). 775-778 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 内藤ゆかり: "キノンプロファイル法による池微生物群集構造と水質との関係解析"第33回水環境学会年会講演要旨集. 220 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 林炳蘭: "キノンプロファイル法による生活排水処理活性汚泥曝気槽内微生物群集の季節変動の解析"第33回水環境学会年会講演要旨集. 245 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 國弘忠生: "土壌・水系におけるキノン組成及び存在比"第33回水環境学会年会講演要旨集. 372 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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