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Development of a cooling device for cryogenic X-ray and neutron Crystallography

Research Project

Project/Area Number 11558086
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biophysics
Research InstitutionThe University of Tokyo

Principal Investigator

NAKASAKO Masayoshi  The Institute of Molecular and Cellular Biosciences, The University of Tokyo, Lecturer, 分子細胞生物学研究所, 講師 (30227764)

Co-Investigator(Kenkyū-buntansha) SAWANO Masahito  Rigaku-denki Corp., System design group, associate engineer, Researcher, システム設計部・機会設計グループ, 技師補(研究職)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1999: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsProteins / Radiation damage / Cryogenic Crystallography / Third-generation synchrotron facility / cryogenic cooling / Cryogenic protein crystallography / X-ray crystallography / 構造生物学 / X線解析 / X線回折 / 放射光X線 / 中性子回折
Research Abstract

An open-flow cryostat using helium gas has been developed for cryogenic X-ray crystallography. The device is composed of a cryostat and a heat exchange apparatus, and dry nitrogen gas is supplied for shielding the cooled helium gas from atmosphere. The lowest temperature reached by the device is 35 K within a fluctuation of ±0.5 K at a flow rate of 6 litter/min. This device is easy to operate and enables to study crystal structures and the temperature-dependent phenomena occurring in biological macromolecules and materials above 35 K. In addition, The techniques in cryogenic experiments around the liquid nitrogen temperature have been applied to the structural determinations of various enzymes, the hydration structure analysis of proteins and the investigation of internal vibrational properties of proteins around the glass-transition temperature.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (70 results)

All Other

All Publications (70 results)

  • [Publications] M.Nakasako: "Structural characteristics in protein hydration investigated by cryogenic X-ray crystal structure analyses"Journal of Biological Physics. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Nakasako, M.Sawano, M.Kawamoto: "An open-flow helium cryostat for cryogenic X-ray diffraction experiments"Review of Scientific Instruments. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Motoyama, M.Nakasako, I.Yamaguchi: "Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37 K"Acta Crystallographica Sect. D. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Nakasako, F.Tsuchiya, Y.Arata: "Roles of hydration water molecules in molecular packing of the killer toxin in its crystalline state investigated by cryogenic X-ray crystallography"Biophysical Chemistry. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Nakasako: "Large-scale networks of hydration water molecules around proteins investigated by cryogenic X-ray crystallography"Cellular and Molecular Biology. 47. 767-790 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] I.Endo, M.Nojiri, M.Tsujimura, M.Nakasako, S.Nagashima, et al.: "Fe-type Nitrile Hydratase"Journal of Inorganic Biochemistry. 83. 247-253 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Nakasako, T.Fujisawa, S.Adachi, T.Kudo, S.Higuchi: "Large-scale domain movements and hydration structure changes in the active-site cleft in unligated glutamate dehydrogenase from Thermococcus profundus"Biochemistry. 40. 3069-3079 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Z.Zhang, D.Lewis, C.Strock, G.Inesi, M.Nakasako, H.Nomura et al.: "Detailed characterization of the cooperative mechanism of Ca^<2+> binding and catalytic activation in the Ca^<2+> transport(SERCA) ATPase"Biochemistry. 39. 8758-8767 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] C.Toyoshima, M.Nakasako, H.Nomura, H.Ogawa: "Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 Åresolution"Nature. 405. 647-655 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Higuchi, M.Nakasako, T.Kudo: "Crystallization and preliminary X-ray diffraction studies of hyperthermostable glutamate dehydrogenase from Thermococcus profundus"Acta Crystallographica Sect. D. 55. 1917-1919 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Nakasako, M.Odaka, M.Yohda, N.Dohmae, K.Takio, N.Kamiya et al.: "Tertiary and quaternary structures of photoreactive Fe-type nitrile hydratase from Rhodococcus sp. N-771 : Roles of hydration water molecules in stabilizing the structures and the structural origin for the substrate specificity of the enzyme"Biochemistry. 38. 9887-9898 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Nakasako, H.Takahashi, N.Shimba, I.Shimada, Y.Arata: "The pH-dependent structural variation of complementarity-determining region H3 in the Fv fragment from an anti-dansyl monoclonal antibody, IgG2a(s)"Journal of Molecular Biology. 291. 117-134 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Nakasako: "Large-scale networks of hydration water molecules around b-trypsin revealed by cryogenic X-ray crystal structure analyses"Journal of Molecular Biology. 289. 547-564 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Sato, K.Takeda, K.Tani, T.Okada, M.Nakasako, N.Kamiya et al.: "Specific lipid-protein interactions in a novel honeycomb lattice structure of bacteriorhodopsin"Acta Crystallographica Sect. D. 55. 1251-1256 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Nakasako, M.Kimura, I.Yamaguchi: "Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus"Acta Crystallographica Sect. D. 55. 547-548 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中迫雅由, 澤野成民, 河本正秀: "低温ヘリウムガス吹き付け型結晶冷却装置の開発"放射光. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Nakasako, M.Sawano, M.Kawamoto: "Development of an open-flow cryostat utilizing helium gas"The Rigaku-Denki Journal. 18. 47-53 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中迫雅由, 澤野成民, 河本正秀: "低温ヘリウムガス吹き付け型結晶冷却装置の開発"理学電機ジャーナル. 32. 8-15 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 豊島近, 中迫雅由, 野村博美, 小川治夫: "筋小胞体カルシウムポンプの構造決定"蛋白質・核酸・酵素. 46. 1374-1380 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 豊島近, 中迫雅由, 野村博美, 小川治夫: "筋小胞体カルシウムポンプの構造決定"放射光. 14. 204-210 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Nakasako, H.Nomura, H.Ogawa, C.Toyoshima: "Crystal structure analyses of membrane proteins : a case study for calcium pump from sarcoplasmic reticulum"The Japanese Journal of Pharmacology. 85. Suppl.I, S1-1 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中迫雅由, 野村博美, 小川治夫, 豊島近: "筋小胞体カルシウムポンプの結晶構造解析"日本結晶学会誌. 42. 478-485 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中迫雅由: "低温蛋白質結晶構造解析"日本結晶学会誌. 41. 47-56 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] C.Toyoshima, M.Nakasako, H.Nomura: "Na/K-ATPase and related ATPase (ed. Taniguchi & Kayama)"Three-dimensional structural study of Ca^<2+>-ATPase from sarcoplasmic reticulum. 193-196 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Nakasako: "Structural characteristics in protein hydration investigated by cryogenic X-ray crystal structure analyses"Journal of Biological Physics. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Nakasako, M. Sawano and M. Kawamoto: "An open-flow helium cryostat for cryogenic X-ray diffraction experiments"Review of Scientific Instruments. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Motoyama, M. Nakasako and I. Yamaguchi: "Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37 K"Acta Crystallographica Sect. D. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Nakasako, F. Tsuchiya and Y. Arata: "Roles of hydration water molecules in molecular packing of the killer toxin in its crystalline state investigated by cryogenic X-ray crystallography"Biophysical Chemistry. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Nakasako: "Large-scale networks of hydration water molecules around proteins investigated by cryogenic X-ray crystallography"Cellular and Molecular Biology. 47. 767-790 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Nakasako, T. Fujisawa, S. Adachi, T. Kudo and S. Higuchi: "Large-scale domain movements and hydration structure changes in the active-site cleft in unligated glutamate dehydrogenase from Thermococcus profundus studied by cryogenic X-ray crystal structure analysis and small-angle X-ray scattering"Biochemistry. 40. 3069-3079 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Endo, M. Nojiri, M. Tsujimura, M. Nakasako, S. Nagashima, M. Yohda and M. Odaka: "Fe-type Nitrile Hydratase"Journal of Inorganic Biochemistry. 83. 247-253 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Z. Zhanq, D. Lewis, C. Strock, G. Inesi, M. Nakasako., H. Nomura and C. Toyoshima: "Detailed characterization of the cooperative mechanism of Ca^<2+> binding and catalyti activation in the Ca^<2+> transport (SERCA) ATPase"Biochemistry. 39. 8758-8767 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] C. Toyoshima, M Nakasako. H. Nomura and H. Ogawa: "Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 Å resolution"Nature. 405. 647-655 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Higuchi, M Nakasako and T. Kudo: "Crystallization and preliminary X-ray diffraction studies of hyperthermostable glutamate dehydrogenase from Thermococcus profundus"Acta Crystallographica Sect.. D 55. 1917-1919 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Nakasako, M. Odaka, M. Yohda, N. Dohmae, K. Takio, N. Kamiya and I. Endo: "Tertiary and quaternary structures of photoreactive Fe-type nitrile hydratase from Rhocdococcus sp. N-771 : Roles of hydration water molecules in stabilizing the structures and the structural origin for the substrate specificity of the enzyme"Biochemistry. 38. 9887-9898 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Nakasako. H. Takahashi, N. Shimba, I. Shimada and Y. Arata: "The pH-dependent structural variation of complementarity-determining region H3 in the Fv fragment from an anti-dansyl monoclonal antibody, lgG2a(s)"Journal of Molecular Biology. 291. 117-134 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Nakasako: "Large-scale networks of hydration water molecules around b-trypsin revealed by cryogenic X-ray crystal structure analyses"Journal of Molecular Biology. 289. 547-564 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Sato, K. Takeda, K. Tani, I Okada, M. Nakasako, N. Kamiya and I Kouyama: "Specific lipid-protein interactions in a novel honeycomb lattice structure of bacteriorhodopsin"Acta Crystallographica Sect. D55. 1251-1256 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Nakasako, M. Kimura and I. Yamaguchi: "Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus"Acta Crystallographica Sect.. D55. 547-548 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Nakasako, M. Sawano and M. Kawamoto: "Development of an open-flow cryostat utilizing helium gas"The Rigaku-Denki Journal. 18. 47-53 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Nakasako, H. Nomura, H. Ogawa and C. Toyoshima: "Crystal structure analyses of membrane proteins: a case study for calcium pump from sarcoplasmic reticulum"The Japanese Journal of Pharmacology. 85 Suppl. l.. S1-1 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] C. Toyoshima, M. Nakasako and H. Nomura: "Three-dimensional structural study of Ca^<2+>-_-ATPase from sarcoplasmic reticulum"Na/K-ATPase and related ATPase (ed. Taniguchi & Kayama). 193-196 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Nakasako: "Structural characteristics in protein hydration investigated by cryogenic X-ray crystal structure analyses"Journal of Biologocal Physics. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中迫雅由, 肥後順一: "水とタンパク質が織りなす生命活動の素過程"現代化学. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Nakasako, F.Tsuchiya, Y.Arata: "Roles of hydration water molecules in molecular packing of the killer toxin in its crystalline state investigated by cryogenic X-ray crystallography"Biophysical Chemistry. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中迫雅由, 澤野成民, 河本正秀: "低温ヘリウムガス吹き付け型結晶冷却装置の開発"放射光. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] J.Higo, M.Nakasako: "Hydration structure of human lysozyme investigated by molecular dynamics simulation and cryogenic X-ray crystal structure analyses"Journal of Computational Chemistry. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Nakasako, M.Sawano, M.Kawamoto: "An open-flow helium cryostat for cryogenic X-ray diffraction experiments"Review of Scientific Instruments. 73. 1318-1320 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Motoyama, M.Nakasako, I.Ymmaguchi: "Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37K"Acta Crystallographica Sect.D. 58. 148-150 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Nakasako, M.Sawano, M.Kawamoto: "Development of an open-flow cryostat utilizing helium gas"The Rigaku-Denki Journal. 18. 47-53 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中迫雅由, 澤野成民, 河本正秀: "低温ヘリウムガス吹き付け型結晶冷却装置の開発"理学電機ジャーナル. 32. 8-15 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Nakasako: "Large-scale networks of hydration water molecules around proteins investigated by cryogenic X-rayc crystallography"Cellular and Molecular Biology. 47. 767-790 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] I.Endo, M.Nojiri, M.Tsujimura, M.Nakasako, S.Nagashima, et al.: "Fe-type Nitrile Hydratase"Journal of Inorganic Biochemistry. 83. 247-253 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Nakasako, T.Fujisawa, S.Adachi, T.Kudo, S.Higuchi: "Large-scale domain movements and hydration structure changes in the active-site cleft in unliated glutamate dehydrogenase from Thermococcus profundus"Biochemistry. 40. 3069-3079 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 豊島近, 中迫雅由, 野村博美, 小川治夫: "筋小胞体カルシウムポンプの構造決定"蛋白質・核酸・酵素. 46. 1374-1380 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 豊島近, 中迫雅由, 野村博美, 小川治夫: "筋小胞体カルシウムポンプの構造決定"放射光. 14. 204-210 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Nakasako, H.Nomura, H.Ogawa, C.Toyoshima: "Clystal structure analyses of membrane proteins:a case study for calcium pump from sarcoplasmic reticulum"The Japanese Journal of Pharmacology. 85(Suppl. I, S1-1). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Nakasako: "Hydration structures of proteins investigated by cryogenic X-ray crystal structure analyses"Cellular and Molecular Biology "Water in Cell" issue . (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Nakasako et al.: "Large-scale domains movements and hydration structure changes in the active-site cleft in unligated glutamate dehychogenase."Biochemistry. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] I,Endo et al.: "Fe-type nitrile hyodratase"Journal of Inorganic Biochemistry. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 中迫雅由 ら: "筋小胞体カルシウムポンプの結晶構造解析"日本結晶学会誌.. 42. 478-485 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Zhang et al.: "Detailed characterzation of the cooperative mechamism of Ca^<2+> binding and catalytic activation in the Ca^<2+> transport (SECA) ATPase"Biochemistry. 39. 8758-8767 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Toyoshima et al.: "Caystal structure of the calgium pump of sarcoplasmic reticulum at 2.6 A resolution"Nature. 405. 647-655 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S. Higuchi: "Crystallization and preliminary X-ray diffraction studies of hyper-thermostable glutamate dehydrogenase from Thermococcus profundus"Acta Crystallographica. D55. 1917-1919 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Nakasako: "Tertiary and quaternary structures of photoactive Fe-Type nitrile"Biochemistry. 38. 9887-9898 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Nakasako: "The pH-dependent suructural variation of complementary-determining region H3 in the Fv fragment"Journal of Molecular Biology. 291. 117-134 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Nakasako: "Lorge-scale networks of hydration water molecules around β-trypsin revealed by cryogenic X-ray crystal structure analyses"Journal of Molecular Biology. 289. 547-564 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H. Sato: "Specific Lipid-protein interactions in a nobel honycomb lattice structure of bacteriorhodopsin"Acta Crystallographica. D55. 1251-1256 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Nakasako: "Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus tereus"Acta Crystallographica. D55. 547-548 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中迫雅由: "低温蛋白質結晶構造解析"日本結晶学会誌. 41. 47-65 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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