| Project/Area Number |
11558090
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Tohoku University |
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Principal Investigator |
OSUMI Noriko Tohoku University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (00220343)
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| Co-Investigator(Kenkyū-buntansha) |
WAKAMATU Yoshio Tohoku University Graduate School of Medicine, Lecturer, 大学院・医学系研究科, 講師 (60311560)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1999: ¥11,300,000 (Direct Cost: ¥11,300,000)
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| Keywords | Neural development / Mammalian embryo / fetus / Manipulation / Ultrasound microscope / In utero operation / Gene transfer / Cortex / GFP |
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| Research Abstract |
Brain functions such as memory, lerning, and emotion, are based on neuronal networks connected among various neurons in specific regions. In order to elucidate molecular mechanisms of brain development, it is required to establish novel techniques to directly manipulate mammalian fetuses of the middle gestation and thereafter at molecular levels. This research project aimed to do the following subjects :
1) Establishment of techniques for manipulating and maintaining mammalian fetuses at the middle gestation and thereafter
2) Establishment of techniques for transplanting cells into the brain of mammalian fetuses at the middle gestation and thereafter
3) Establishment of techniques for transferring exogenous genes into the brain of mammalian fetuses at the middle gestation and thereafter
The following results are obtained in the period supported by this grant :
1) As a technique for manipulating and maintaining mammalian fetuses at the middle gestation and thereafter, we established ultrasound-guided in utero manipulation, thereby operated fetuses being able to survive and develop at the full term.
2) As preliminary experiments for cell transplantation and gene transfer, we tried to inject dye solutions into the operated fetuses.
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