| Project/Area Number |
11558113
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
MIKUNI-TAKAGAKI Yuko Kanagawa Dental College, Faculty of Dentistry, Lecturer, 歯学部, 講師 (60050689)
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| Co-Investigator(Kenkyū-buntansha) |
SATOYOSHI Masanori Kanagawa Dental College, Faculty of Dentistry, Research Associate, 歯学部, 助手 (90257303)
OOTA Tomohiro Teijin Limited, Manager, 課長(研究職)
ITOMAN Moritoshi Kitasato University, Faculty of Medicine, Professor, 医学部, 教授 (70104528)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1999: ¥8,200,000 (Direct Cost: ¥8,200,000)
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| Keywords | osteocytes / ultrasound / osteopenia / disuse / synergy / mechanotransduction / bone formation / crosstalk / 運動負荷 / 機械的刺激 / 骨萎縮 / 骨 / 骨芽細胞 / 骨髄間質細胞 |
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| Research Abstract |
Anabolic response to low-intensity, pulsed ultrasound and the associated mechanotransduction pathways were studied in rat bone marrow cells. Effects of 20-min exposure to low intensity, pulsed ultrasound were examined in both primary rat bone marrow cells and mouse bone marrow derived ST2 cells. The marrow-derived cells cultured in the presence and absence of ascorbate were treated with Dexamethazone for 1, 7, and 14 days prior to the exposure to the ultrasound so that their responses at different developmental stages can be distinguished. We determined the steady state levels of IGF-I, osteocalcin and other bone protein mRNAs including immediate early genes such as c-fos or cox 2 by using RT-PCR analysis. Upregulation of the mRNA levels that we have reported in ST2 cells (BBRC 268 : 216, 2000) was reproducible only under limited conditions, suggesting that the low intensity, pulsed ultrasound induce anabolic responses in cells only at early stages of osteogenic lineage. Our findings indicate that the direct reaction of certain population of cells to low intensity, pulsed ultrasound influences other cells in a paracrine manner resulting in acceleration of various phases of fracture repair.
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