| Project/Area Number |
11558115
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
OGASAWARA Yasuo Kawasaki Medical School, Medical Engineering, Associate Professor, 医学部, 助教授 (10152365)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMOTO Hiroshi Kawasaki Medical School, Medical Engineering, Research Associate, 医学部, 助手 (10299183)
YADA Toyotaka Kawasaki Medical School, Medical Engineering, Assistant Professor, 医学部, 講師 (00210279)
KAJIYA Fumihiko Okayama University Medical School, Professor, 医学部, 教授 (70029114)
YAMAMORI Shinji Nihon Kohden Co., R&D Center, Manager, R&Dセンター, 部長
TOYOTA Eiji Kawasaki Medical School, Nuclear Medicine Research Associate, 医学部, 助手 (30248215)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2000: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1999: ¥8,200,000 (Direct Cost: ¥8,200,000)
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| Keywords | microstrain / NADH / ischemia / coronary microcirculation / high speed CCD microscope / マクロストレイン |
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| Research Abstract |
The major size of a vascular bed that a minimum arteriole governs within myocardium is several hundreds mm long. At this level of size, the heterogeneity of coronary blood-flow distribution is remarkable. However, it is unknown whether the heterogeneity of a coronary blood-flow distribution relates to the myocardial micro-deformation in a beating heart.
We have recently developed a micro-light markers implantation method onto the cardiac surface of anaesthetized open-chest dogs and directly observed the myocardial micro-deformation in hundreds micrometer order using our newly developed high-magnified(×200), high-speed(200 frames/sec)CCD intravaital videomicroscope. In comparison with this, deformation of larger scale(millimeter order sample size)was also analyzed using an intra-vital microscope with lower magnification(×50). Although the myocardial deformation by cardiac contraction in a macrodomain was comparatively uniform, deformation in a micro-domain showed heterogeneity.
In addition, measurement and analysis of oxygen metabolism image of the isolated rat heart were done using a NADH fluorescence method. The average size of the heterogeneous myocardial deformation unit and patchy NADH fluorescence unit betraying mayocardial oxygen metabolism were in the same order. Furthermore, trasitional NADH activity at myocardial wall section was observed between epimyocardium and endomyocardium. The time constant of appearance and disappearance of NADH fluorescence revealed that endomyocardium is much more vulnerable to hypoxia than epimyocardium.
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