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New approach for drug monitoring by using human sweat secreted

Research Project

Project/Area Number 11559008
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 広領域
Research InstitutionNagoya Institute of Technology

Principal Investigator

TSUDA Takao  Nagoya Institute of Technology, Prof., 工学部, 教授 (50023156)

Co-Investigator(Kenkyū-buntansha) ISHII Akira  Nagoya University School of Medicine, Associate Prof., 医学部, 助教授 (30252175)
SUZUKI Osamu  Hamamatsu University School of Medicine, Prof., 法医学講座, 教授 (70093044)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1999: ¥5,700,000 (Direct Cost: ¥5,700,000)
KeywordsSweat / drug / drug monitoring / noninvasive / theophylline / metabolites / easy sampling / monitoring at bed side / 簡易サンプリング / カフェイン / 咳止め薬 / 代謝 / 直接サンプリング
Research Abstract

Human sweat is one of the body fluids. Human sweat is secreted on a skin surface, so that it is easy to collect non-invasively. We intend to collect human sweat by handy sampling method and to use it for drug monitoring. First we developed the handy sampling method of sweat secreted and simultaneously we measured the amount of sweat. The handy method of sampling was archived by using a small vial (inner volume 0.6 ml) containing few percentage alcohol-water solution. The vial attached on the surface of human skin for 3 to 5 min for collecting sweat. Then the component in sweat was analyzed by liquid chromatograph, capillary electrophoresis, GC/MS and etc.
1.The variation of caffeine in sweat was measured after intake of a tablet containing caffeine. Caffeine and its metabolites were found in sweat and their concentrations were varied according to the elapsed period.
2. Codeine was also found after intake of a medicine.
3. Organic carboxylic acids and glucose were founded in sweat and their concentration variation were also studied after their intakes.
4. Volatile organic gases are directly collected from skin surface. The gases was led to cold trap for 30 second, and switched to analysis by gas chromatography. New devices of trapping has been investigated and applied for international patents.
5. Inorganic anions also investigated.
From above studies, we concluded it is possible to use sweat for drug monitoring, because it contains a lot of components that have a certain relationship with the concentration in blood. The use of sweat is very attractive because it is easy to adopt for testing of body conditions by people for their control of daily life.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] Ken Naitoh: "Direct Temperature-controlled Trapping system and its use for the gas chromatographic determination of Organic vapor released from human skin"Analytical Chemistry. 72. 2797-2801 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takao Tsuda: "Proposal of sampling process for collecting human sweat and determination of caffeine concentration in it by using GC/MS"Biomedical Chromatography. 14. 505-510 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 津田孝雄: "カフェイン錠剤服用後の-汗中カフェイン及びその代謝物の存在確認とそれらの濃度経時変化"発汗学. 8. 13-19 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takao Tsuda: "Noninvasive approach for study of human reaction and transferring velocity of nervous instrumentation devised for concurrent microscopic observation and analysis of sweat secretion on human body under 1G and microgravity"Journal of gravitational Physiology. 7. 129-130 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 増村年章: "精神疾患患者におけるボールキャッチ時の発汗反応"発汗学. 7. 85-88 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 大桑哲男: "運動負荷中の汗中乳酸量のモニタリング"発汗学. 8. 47-49 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 寺澤純子: "キャピラリー電気泳動法によるヒト汗中のナトリウム、カリウム、カルシュム、マクネシュム、アンモニウムイオンの定量"分析化学. 50. 813-817 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ken Naitoh: "New measurement of hydrogen gas and acetone in gases emanating from human skin"Instrumental Science & Technology. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 増村年章: "精神分裂症におけるボールキャッチ時の発汗反応-健常対照者との比較-"発汗学. 9. 25-28 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] "Exhalation behavior of four organic substrates and water absorbed by human skin"Biological & Pharmaceutical Bulltin. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takaharu Kondou: "Assessment of colonic fermentation by hydrogen release from skin"The American J. Gastroenterology. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 津田孝雄: "楽しい化学実験"丸善. 154 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 津田孝雄: "ヒトの発汗活動と汗に含まれる化学物質から分かること(化学への招待)"日本化学会東海支部化学教育協議会. 16-29 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ken Naitoh, Yoshihito Inai, Tadamichi Hirabayashi, and Takao Tsuda: "Direct Temperature-controlled Trapping system and its use for the gas chromatographic determination of Organic vapor released from human skin"Analytical Chemistry. 72. 2797-2801 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takao Tsuda, Saeko Noda, Shinya Kitagawa, and Toru Morishita: "Proposal of sampling process for collecting human sweat and determination of caffeine concentration in it by using GC/MS"Biomedical Chromatography. 14. 505-510 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takao Tsuda, Naohiro Naito, Nao Mizuno, Shinya Kitagawa, and Akira Ishii: "Determination of Caffeine and its metabolites in sweat secreted after intake of caffeine, and their time variation (in Japanese)"Japanese Journal of perspiration. 8. 13-19 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Tsuda, S.Kitagawa, and S.Nagaoka: "Noninvasive approach for study of human reaction and transferring velocity of nervous instrumentation devised for concurrent microscopic observation and analysis of sweat secretion on human body under 1G and microgravity"Journal of gravitational Physiology. 7. 129-130 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tetsuo Okuwa, Sadaharu Sugase, Youhei Tachi, Takako Yamamoto, Shinya Kitagawa, and Tsuda Takao: "Monitoring of concentration of lactic acid in human sweat secreted during excersice by using a newly devised-handy sampling"Japanese Journal of perspiration. 8. 47-49 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Junko Terasawa, Kotaro Mitsui, Akira Ishii, Takao Tsuda: "Quantitative determination of K^+, NH4^+, Ca^+, and Mg^+ cations in secreted human sweat by capillary zone electrophoresis"Bunseki Kagaku. 50. 813-817 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ken Naitoh, Yoshiki Inai, Tadamichi Hirabayashi, Takao Tsuda: "Exhalation behavior of four organic substrates and water absorbed by human skin"Biological & Pharmaceutical Bulletin. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ken Naitoh, Takao Tsuda, Kazutodshi Nose, Takaharu Kondo, Akinori Takasu, and Tadamichi, Hirabayashi: "New measurement of hydrogen gas and acetone in gases emanating from human skin"Instrumental Science & Technology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takaharu Kondou, Takao Tsuda, Hirotoshi Ishiguro, Takahiro Mitsui, Kai-Ping Gao, and Kotoya Fujiki: "Assessment of colonic fermentation by hydrogen release from skin"The American J. Gastroenterology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hideki Shibata, Takao Tsuda: "Determination of paraben found on skin surface of human finger"Bunseki Kagaku. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ken Naitoh: "Direct Temperature-controlled Trapping system and its use for the gas chromatographic determination of Organic vapor released from human skin"Analytical Chemistry. 72. 2797-2801 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takao Tsuda: "Proposal of sampling process for collecting human sweat and determination of caffeine concentration in it by using GC/MS"Biomedical Chromatography. 14. 505-510 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 津田孝雄: "カフェイン錠剤服用後の一汗中カフェイン及びその代謝物の存在確認とそれらの濃度経時変化"発汗学. 8. 13-19 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takao Tsuda: "Noninvasive approach for study of human reaction and transferring velocity of nervous instrumentation devised for concurrent microscopic observation and analysis of sweat secretion on human body under IG and microgravity"Journal of gravitational Physiology. 7. 129-130 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 増村年章: "精神疾患患者におけるボールキャッチ時の発汗反応"発汗学. 7. 85-88 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 白井良和: "飲酒による汗中アルコール及び発汗量の変動とヒトスジシマカ誘引との相関に関する検討"発汗学. 6. 68-71 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] 大桑哲男: "運動負荷中の汗中乳酸量のモニタリング"発汗学. 8. 47-49 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 寺澤純子: "キャピラリー電気泳動法によるヒト汗中のナトリウム、カリウム、カルシュム、マグネシュム、アンモニウムイオンの定量"分析化学. 50. 813-817 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 津田孝雄: "楽しい化学実験"丸善. 154 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ken Naitoh: "Direct Temperature-controlled Trapping system and its use for the gas chromatographic determination of Organic vapor released from human skin"Analytical Chemistry. 72. 2797-2801 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takao Tsuda: "Proposal of sampling process for collecting human sweat and determination of caffeine concentration in it by using GC/MS"Biomedical Chromatography. 14. 505-510 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 津田孝雄: "カフェイン錠剤服用後の-汗中カフェイン及びその代謝物の存在確認とそれらの濃度経時変化"発汗学. 8. 13-19 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takao Tsuda: "Noninvasive approach for study of human reaction and transferring velocity of nervous instrumentation devised for concurrent microscopic observation and analysis of sweat secretion on human body under 1G and microgravity"Journal of gravitational Physiology. 7. 129-130 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 増村年章: "精神疾患患者におけるボールキャッチ時の発汗反応"発汗学. 7. 85-88 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 白井良和: "飲酒による汗中アルコール及び発汗量の変動とヒトスジシマカ誘引との相関に関する検討"発汗学. 6. 68-71 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 津田孝雄 編: "楽しい科学実験 Part2"丸善. 160 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 津田孝雄: "微小部位の発汗活動と神経伝達速度の計測"発汗学. 6. 19-22 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Naito,Y.Inai,T.Hirabayashi,T.Tsuda: "Direct temperature-controlled trapping system,and its use for gas chrom.determ.of org,vapor released from human skin"Analytical Chemistry (Accept 見込み).

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Tsuda,S.Nada,S.Kitagawa T.Morishita: "Capillary gas chromatographic determination of caffeine cmc.in human sweat and proposal of novel sampliry method"Biomedical Chromatography(Accept見込み).

    • Related Report
      1999 Annual Research Report
  • [Publications] 白井,関,諸橋,北川,津田: "飲酒による汗中アルコールおよび発汗量の変動とヒストジシマカ誘引との相関に関する検討"発汗学. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] 津田 孝雄,内藤 健: "ヒト皮膚からの揮発成分の捕集方法"国有特許申請. (手続中).

    • Related Report
      1999 Annual Research Report
  • [Publications] 津田孝雄編集: "楽しい科学実験 パートII"丸善(2000年9月予定). (200)

    • Related Report
      1999 Annual Research Report
  • [Publications] 津田孝雄他編集: "キラル分離(仮題)"学術出版(2000年9月予定). (250)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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