| Project/Area Number |
11559014
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
広領域
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| Research Institution | Hiroshima University |
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Principal Investigator |
MICHIBATA Hitoshi Hiroshima Univ., Graduate School of Science, Professor, 大学院・理学研究科, 教授 (00111740)
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| Co-Investigator(Kenkyū-buntansha) |
UEKI Tatsuya Hiroshima Univ., Grad. Sch. Sci., Research Associate, 大学院・理学研究科, 助手 (10274705)
UYAMA Taro Hiroshima Univ., Grad. Sch. Sci., Lecturer, 大学院・理学研究科, 講師 (60232914)
KANAMORI Kan Toyama Univ., Fac. Sci., Professor, 理学部, 教授 (00019001)
HIROTSU Takahiro Nat. Inst, Adv. Ind. Sci. Tech., Shikoku Center, Stuff, 四国センター・海洋資源部・生物材料研究室, 室長
大井 健太 工業技術院, 四国工業技術研究所・海洋資源部・企画課, 課長
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2001: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2000: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1999: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | Ascidian / Vanadium / Transition metals / Highly selective accumulation / Vanadium-binding proteins / Rare metals / 金属イオン / 海水 / 選択的分取 / 吸着剤 / 遺伝子クローニング |
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| Research Abstract |
Rare metals such as vanadium, cobalt, manganese, nickel and molybdenum are dissolved in sea water at very low levels. Ascidians, especially belonging to the Phlebobranchia, are known to selectively accumulate high levels of vanadium. We previously extracted vanadium-associated proteins from vanadium-containing blood cells (vanadocytes) of Ascidia sydneiensis samea. In this study, full-length of cDNAs, encoding 12.5 kDa and 15 kDa vanadium-associated proteins (designated newly as Vanabin), respectively, which might have a clue to resolve the mechanism of vanadium accumulation, was analyzed. As a result, Vanabin was disclosed to consist of about 120 amino acids in which the content of cysteine residues is very high and to be a novel protein having α-helix. Recombinant Vanabin was found to bind with about 20 atoms of vanadium ions and its binding constant is 10^<-7>M. Metal binding sites in progress.
Recently, it has been disclosed that 12.5 kDa Vanabin binds with about 10 atoms of vanadium in the +4 oxidation state, 15 kDa Vanabin does not bind with iron ions or copper ions and copper ions inhibits the binding of Vanabin with vanadium ions, competitively.
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