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Characterization of the mus-10 and recQ genes involving in DNA repair, recombination, and senescence

Research Project

Project/Area Number 11640619
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionSaitama University

Principal Investigator

INOUE Hirokazu  SAITAMA University, Fac. Science Dept. of Regulation-Biology, Professor, 理学部, 教授 (60114203)

Co-Investigator(Kenkyū-buntansha) ISHII Chizu  SAITAMA University, Fac. Science Dept. of Regulation-Biology, Associate Professor, 理学部, 助教授 (00114215)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsrecQ / DNA repair / Recombination / Neurospora / queling / mitochondria / Instability / aging / mus-10 / ミトコンドリアDNA / DNAシークエンス
Research Abstract

Neurospora crassa has 2 recQ homolog genes, recQ1 and recQ2, which encode RecQ helicase. We disrupted those genes and characterized phenotypes. Gene recQ1 was same to mus-19 and also qde-3 genes. The mus-19 mutant is sensitive to MMS. EMS, MNNG, 4NQO and camptotecin. Epistasis analyzes showed that mus-19 belonged to recombination repair and postreplication repair. It did not show high mutation frequency and high recombination frequency. In the mus-19 strain we could not identify "quering" that is gene silencing in N. crassa. Also aging of the hyphae was not observed in this strain. Like the mus-19 strain, the recQ2 mutant was sensitive to mutagens. We are investigating other phenotype of this strain.
The mus-10 mutant grow normally after germination, but several days later the growth became slow and finally stopped. This strain was sensitive UV and MMS. Epistasis analyzes showed that combination with mus-18 become synergistic in mutagen sensitivity, but other combination did not. Telomere length did not change at the aging step. Some data showed this mutant had defect in mitochondria DNA stability.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] A.Kato, et al.: "Cloning and charaterization of a RECQ homolog in Neurospora crassa"Gene & Genetic Systems. 74. 329 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Sawada, et al.: "Analysis of the mutagen-sensitive mutant, mus-10, in Neurospora crassa"Gene & Genetic Systems. 74. 329 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A.Kato, et al.: "Characterization of a recQ homolog in Neurospora crassa"Gene & Genetic Systems. 76. 389 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kato, A., Y. Akamatsu, Y. Sakuraba, H. Inoue: "Cloning and characterization of a RECQ homologue in Neurospora crassa"Genes & Genetic System. 74 (6). 329 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sawada, S., M. Kobori, S. Hatakeyama, H. Inoue.: "Analyses of the mutagen-sensitive mutant, mus-10, in Neurospora crassa"Genes & Genetic Systems. 74 (6). 329 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kato, A., Y. Akamatsu, Y. Sakuraba H. Inoue: "Characterization of a recQ homologue in Neurospora crassa : involvement in recombination and gene silencing"Genes & Genetic Systems. 76 (6). 389 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary

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Published: 1999-04-01   Modified: 2016-04-21  

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