| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Research Abstract |
1. Although transcription of the late competence genes was not detected in the B.subtilis natto strain OK2 during competence development, these genes were constitutively transcribed in the OK2 strain carrying either the mecA or clpC mutation. Both OK2 mutants exhibited high transformation frequencies, comparable with that observed for B.subtilis 168.
2. The sigA21 mutation caused a single-amino acid substitution, E314K, in region 4 of the σ^A protein. In this mutant, expression of the spoIIG gene, whose transcription depends on both and Spo0A〜P, was greatly reduced at 43℃. A spontaneous sporulation-proficient suppressor mutant at 43℃ was isolated. This suppressor mutation was mapped within the rpoB gene, encoding β subunit of RNA polymerase, and was found to have a single-amino acid substitution, A863G.
3. The expression of the spo0A and spoIIG genes, was significantly decreased in the clpP mutant at the onset of sporulation. The σ^H and Spo0A protein levels in the clpP mutant were also decreased during the initiation of sporulation, indicating that the accumulation of Spo0A〜P was inhibited in the clpP mutant.
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