• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Re-examination on two different biosynthetic pathways of galactolipids in higher plants using molecular cell biological technique.

Research Project

Project/Area Number 11640644
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionTokyo Institute of Technology

Principal Investigator

OHTA Hiroyuki  Tokyo Institute of Technology, Associate Professor, 大学院・生命理工学部, 助教授 (20233140)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsChloroplast / Glycolipid / Glycosyltransferase / Thylakoid / Monogalactosyldiacylglycerol / モノガラクトシルジアシルブリセロール / モノガラクトシルジアシルグリセロール
Research Abstract

1) Three different types of MGDG synthase genes (AtMGD1, AtMGD2, AtMGD3) were identified in Arabidopsis thaliana.
2) Tissue specificity of gene expression for three MGDG synthase genes was determined.
3) Enzymatic property of three MGDG synthases was determined.
4) Subcellular localization of three MGDG synthases was determined.
5) According to the results described above, we classified three MGDG synthases into two types, namely type A (AtMGD1) and type B (AtMGD2, AtMGD3). Role of the two types in two different biosynthetic pathways in galactolipid synthesis was discussed, and their distinct roles were proposed.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Koichiro Awai et al.: "Two type of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana"Proc. Natl. Acad. Sci. USA. 98. 10960-10965 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Muhammed Gad et al.: "Accumulation of Plant Galactolipid Affects Cell Morphology of Esherichia celi"Biochem. Biophys. Res. Comm.. 286. 114-118 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroyuki Ohta et al.: "Glyceroglycolopids of photosyythetic organisms-Their biosynthesis and evolutionary origin-"Trends in Glycoscience and Glycotechnology. 12. 241-253 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Eric Marechal et al.: "The multigenic family of monogalactosyldiacylglycerol synthasis"Biochem. Soc. Trans.. 28. 732-738 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshiki Yamaryo et al.: "Transcriptional regulation by light and phytohormones of the MGD gene in cluculmber"Biochem. Soc. Trans.. 28. 738-740 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 太田啓之: "ようやく分かってきた高等植物の糖脂質合成系"化学と生物. 38. 37-39 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Awai, K., Marechal, E., Block, M.A, Brun, D., Masuda, T., Shimada, H., Takamiya, K., Ohta, H., Joyard, J.: "Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana"Proc. Natl. Acad. Sci., USA. 98(19). 10960-10965 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Gad, M., Awai, K., Shimojima, M., Yamaryo, Y., Shimada, H., Masuda, T., Takamiya, K., Ikai, A., Ohta, H.: "Accumulation of plant galactolipid affects cell morphology of Escherichia coli"Biochem. Biophys. Res. Comm.. 286. 114-118 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohta, H., Awai, K. and Takamiya, K.: "Glyceroglycolipids of photosynthetic organisms Their biosynthesis and evolutionary origin"Trends In Glycoscience and Glycotechnology. 12(66). 241-253 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamaryo, Y., Kanai, D., Awai, K., Masuda, T., Shimada, H., Takamiya, K., Ohta, H.: "Transcriptional regulation by light and phytochromes of the MGD gene in cucumber"Biochem. Soc. Trans.. 28. 738-740 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Marechal E, Awai K, Block MA, Brun D, Masuda T, Shimada H, Takamiya K, Ohta H, Joyard J.: "The multigenic family of monogalactosyl diacylglycerol synthases"Biochem. Soc. Trans.. 28(6). 732-738 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroyuki Ohta et al.: "Glyceroglycolipids of photosynthetic organisms-their biosynthesis and evolutionary origin"Trends in Glycosci.and Glycotech.. 12(66). 241-253 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Christine Miege: "Biochemical and topological properties of type A MGDG synthase,a spinach chloroplast envelope enzyme catalyzing the synthesis of both prokaryotic and eukaryotic MGDG"Eur.J.Biochem.. 265. 990-1001 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hiroyuki Ohta: "Advances in Plant Lipid Research"Secretariado de Publicaciones,Universidad de Sevilla. 723 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Christine Miege: "Advances in Plant Lipid Research"Secretariado de Publicaciones,Universidad de Sevilla. 723 (1998)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi