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Impurity effects of protein oligomer for protein crystallization and crystal quality

Research Project

Project/Area Number 11650023
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied materials science/Crystal engineering
Research InstitutionRitsumeikan University

Principal Investigator

NAKADA Toshitaka  Ritsumeikan Univ., Fac., Science and Engineering, Associate Professor, 理工学部, 助教授 (20237308)

Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
KeywordsAtomic force microscopy / Protein / Growth step / Fluorescent / Lysozyme / Impurity / crystal
Research Abstract

In this study, impurity effects on the growth of protein (hen egg white lysozyme) crystals have been studied using in-situ atomic force microscopy (AFM) and fluorescent microscopy (FM). The main results are as follows;
1. Development of solution exchange system for AFM observations.
We successively developed a solution exchange system to observe adsorption process of protein molecules by using AFM.
2. Observation of adsorption process of protein on crystal surfaces.
The adsorption of (pure) lysozyme to crystal surfaces has been directly observed by using the system described above.
3. Morphology changes of protein crystals induced by a protein impurity
We observed surfaces of tetragonal lysozyme crystals contaminated by ovotransferrin, which has rather larger molecular weight than that of lysozyme by AFM, and revealed that ovotransferrin preferentially adsorbs on the (101). FM observation also showed that the density of the impurities near the (101) surface is relatively higher than the other region. These results explain the morphological changes of protein crystals induced by the lysozyme crystals.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 内山幸昌, 水谷武臣, 中田俊隆: "原子間力顕微鏡によるタンパク質吸着過程のその場観察"立命館大学理工学研究所紀要. 60. (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Uchiyama, T. Mizutani and T. Nakada: "In-situ observation of protein adsorption process by atomic force microscopy"Memories of the institute of Science & engineering Ritsumeikan Univ. Shiga, Japan. 60. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 内山幸昌, 水谷武臣, 中田俊隆: "原子間力顕微鏡によるタンパク質吸着過程のその場観察"立命館大学理工学研究所紀要. 61. (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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