| Project/Area Number |
11650023
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied materials science/Crystal engineering
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
NAKADA Toshitaka Ritsumeikan Univ., Fac., Science and Engineering, Associate Professor, 理工学部, 助教授 (20237308)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Atomic force microscopy / Protein / Growth step / Fluorescent / Lysozyme / Impurity / crystal |
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| Research Abstract |
In this study, impurity effects on the growth of protein (hen egg white lysozyme) crystals have been studied using in-situ atomic force microscopy (AFM) and fluorescent microscopy (FM). The main results are as follows;
1. Development of solution exchange system for AFM observations.
We successively developed a solution exchange system to observe adsorption process of protein molecules by using AFM.
2. Observation of adsorption process of protein on crystal surfaces.
The adsorption of (pure) lysozyme to crystal surfaces has been directly observed by using the system described above.
3. Morphology changes of protein crystals induced by a protein impurity
We observed surfaces of tetragonal lysozyme crystals contaminated by ovotransferrin, which has rather larger molecular weight than that of lysozyme by AFM, and revealed that ovotransferrin preferentially adsorbs on the (101). FM observation also showed that the density of the impurities near the (101) surface is relatively higher than the other region. These results explain the morphological changes of protein crystals induced by the lysozyme crystals.
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