Study on Influence of Zeta-Potentials of Solute and Membrane on Electro-Ultrafi Itration of Protein Solutions
Project/Area Number |
11650802
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
反応・分離工学
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Research Institution | Oyama National Colleg of Technology |
Principal Investigator |
YOSHIDA Hiroshi Oyama National College of Technology MateriaIs Chemistry and Bioengineering, Professor, 物質工学科, 教授 (30042542)
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Project Period (FY) |
1999 – 2001
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Project Status |
Completed (Fiscal Year 2001)
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Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
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Keywords | Electro-Ultrafi Itration / Ultrafi Itration Membrane / Protein Solution / Zeta-Potential / Electric Power Appl ications / Filtration Cake Resistance / Permeate Flux / Solute Rejection / 限外濾過流速 / ゼーダ電位 |
Research Abstract |
1. Bovine serumalbumin (BSA) and haemoglobin were used as the experimental material for protein solution. They have almost the same molecular weight (MW) but isoelectric points (IP) of them, in which zeta-potential representing surface electric charge of protein is zero, were different : MW=67,000, IP=4.8 for BSA, and MW=68,000.IP=6.8 for haemoglobin. For ultrafiItration membrane, a polymeric membrane of which MW cut off is 50,000 was used. The zeta-potentials of proteins and membrane were investigated with varying pH of protein solution and liquid permeating through the membrane. UltrafiItaration and electro-ultrafiItration were discussed on the basis of the zeta-potential data. 2. Using a dead-end filtration experimental apparatus, ultrafiItration was performed experimentally by varying pH of protein solution, and the flow rate for cake filtration, membrane permeate flux and solute rejection were investigated. The filtration characteristics were almost related with the zeta-potential o
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f protein only : the filtration rate and the permeate flux were the smallest in the IP of each protein solution and were nearly the same in the IPs in both prot-Bin solutions, and they were increased with increasing the absolute value of zeta-potential irrespective of the polarity of it. The process of ultra-filtrationwashardly in fluenced by the zeta-potential of membrane. 3. An experimental apparatus for electro-ultrafiItration was made, and the process of electroultrafiItration of BSA solution was investigated under the operation of constant voltage applied. Under D.C. electric field, the fiItration rate was increasedwith increasing the voltage, because electro-phoresis caused by the zeta-potential of protein was enhanced under higher electric field, but there was no electrical effect in the IP. In the rangeofexperimental conditions used, under A.C. field of whichthe polarityof electrodes is reversed, thefiItration ratewas recoveredperiodicallybutsiightlydecreasedwiththetime. Intermittent power application also was not available for improving electro-ultrafiItration. Less
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Report
(4 results)
Research Products
(3 results)