| Project/Area Number |
11650819
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | Hiroshima University |
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Principal Investigator |
KURODA Akio Hiroshima University, Graduate School of Advanced Sciences of Matter, Associate Professor, 大学院・先端物質科学研究科, 助教授 (50205241)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Tsukasa Hiroshima University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (40151295)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | hydroxyapatite / phosphate / Calcium / polyphosphate / biomineralization / proteolipid / dental calculus / 歯垢 / ポリリン酸 / サイクロデキストリン / リン酸化 |
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| Research Abstract |
Corynebacterium matruchotii is a microbe isolated from dental calculus and involved in the calculus formation mainly composed of hydroxyapatite. This microbe is very important for analyzing mechanism of the calcification process. We have purified proteolipid capable of binding eight molecules of calcium ion (Ca^<2+>) from chloroform : methanol lipid extracts It is likely that the initial binding of Ca^<2+> with this proteolipid promotes inorganic phosphate association and eventually hydroxyapatite formation. A PCR-amplified DNA fragment encoding this proteolipid gene (cplX) was inserted into a p-BAD plasmid and then introduced into Escherichia coli. The CplX protein was successfully expressed in E.coli, but the recombinant was not calcified. Removal of covalently attached acyl moieties by methanolic KOH decreased its ability to form Ca-phospholipid. To analyze the differences in acylation sites of CplX in C.matruchotii and E.coli would be very important for further study of the calcification process.
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