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Basic study on mass production of therapeutic proteins for serious disease by genetically engineered rice cell

Research Project

Project/Area Number 11650821
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物・生体工学
Research InstitutionOsaka Prefecture University

Principal Investigator

TERASHIMA Masaaki  Osaka Prefecture Univ., Chem.Eng., Associate Prof., 工学研究科, 助教授 (30172092)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Hiroyuki  Osaka Prefecture Univ., Chem.Eng., Professor, 大学院・工学研究科, 教授 (50081360)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordsrice cell / recombinant protein / antitrypsin / high density culture / specific growth rate / continuous culture / secretion / therapeutic protein / 植物細胞培養 / α_1-アンチトリプシン / 組換えタンパク質 / 治療用タンパク質 / 遺伝子組換え / ヒトタンパク質
Research Abstract

The following significant results were obtained from the research on production of therapeutic protein, α_1-antitrypsin (AAT) by genetically engineered rice cell.
(1) Growth rate of genetically engineered cell was the same as that of non-transformed rice cell. Specific growth rate of rice cell, 0.3 d^<-1>, was of the same order of magnitude as those of other plant cells.
(2) Oxygen transfer coefficient K_La was 7-8 h^<-1>, and aeration rate did not affect the growth rate of rice cell.
(3) Ammonium was produced at cell growth phase and AAT production phase. However, ammonium did not affect the AAT productivity.
(4) Adjustment of osmotic pressure between growth medium and production medium has favorable effective for AAT production.
(5) AAT productivity was tripled using pyrvic acid as an altemative carbon source.
(6) Continuous production was achieved in the continuous stirred tank reactor.
(7) Cell density was increased up to 14 g-dry cell/L in the batch-type reactor.
(8) Since maximum productivity of AAT at optimal condition was 25 mg/g-dry cell, at least 350 mg/L of AAT can be produced in batch-type reactor.
The recombinant proteins can be rapidly induced by sugar depletion in the employed expression system. Our research strongly suggests that protein production system more efficient than microbial system can be constructed by the rice cell culture system. We hope the mass production system using plant cell culture can be achieved by exploring process-engineering research based on this fundamental research.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] M.Terashima,Y.Ejiri,N.Hashikawa H.Yoshida: "Effect of osmotic pressure on human α_1-antitrypsin production by plant cell culture"Biochemical Engineering Journal. 4・1. 31-36 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Terashima,Y.Ejiri,N.Hashikawa H.Yoshida: "Effects of sugar concentration on recombinant human al-antitrypsin production by genetically engineered rice cell"Biochemical Engineering Journal. 6・3. 201-205 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Terashima, Y.Ejiri, N.Hashikawa, H.Yoshida: "Effect of osmotic pressure on human α_1-antitrypsin production by plant cell culture"Biochem.Eng.J.. 4-1. 31-36 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Terashima, Y.Ejiri, N.Hashikawa, H.Yoshida: "Effect of sugar concentration on recombinant human α_1-antitrypsin production by egnetically engineered rice cell"Biochem.Eng.J.. 6-3. 201-205 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Terashima,Y.Ejiri,N.Hashikawa H.Yoshida: "Effect of osmotic pressure on human α_1-antitrypsin-production by plant cell culture"Biochemical Engineering Journal. 4・1. 31-36 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Terashima,Y.Ejiri,N.Hashikawa,H.Yoshida: "Effects of sugar concentration on recombinant human al-antitrypsin production by genetically engineered rice cell"Biochemical Engineering Journal. 6・3. 201-205 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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