| Project/Area Number |
11650835
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
工業分析化学
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
KUSU Fumiyo Tokyo University of Pharmacy and Life Science, School of Pharmacy, Professor, 薬学部, 教授 (70057371)
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| Co-Investigator(Kenkyū-buntansha) |
ARAI Kensuke Tokyo University of Pharmacy and Life Science, School of Pharmacy, Associate Professor, 薬学部, 講師 (10266885)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Electrochemiluminescence / Flow-through cell / High-sensitive determination / Luminol / Hydrogen peroxide / Glucose / Uric acid / Immobilized enzyme |
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| Research Abstract |
A system containing a flow-through electrochemiluminescent detector was developed for high-sensitive determination of biologically important substances. The main contents of the results are as follows :
1. Construction of the electrochemiluminescent detection system
The system developed was fabricated from a carrier solution reservoir, degasser, pump, injector of test solution, electrochemiluminescent cell and phototube. The experimental conditions, such as the composition of carrier solution, the size and length of Teflon tube for the flow line and the cell design, were optimized to give reproducible data with high sensitivity.
2. High-sensitive determination of hydrogen peroxide
Determination of hydrogen peroxide was carried out using luminol, an electrochemiluminescent reagent. Hydrogen peroxide amount ranging from 59 fmol to 59 nmol could be determined using 5.0 μM luminol in a phosphate buffer (pH 7.6) at a flow rate of 0.4 ml/min and an applied potential of 1.1 V vs. Ag/AgCl.
3. Analysis of serum
As examples of analysis of biological samples, glucose and uric acid in serum were determined. Glucose oxidase or uricase were immobilized on Chitopearl filled in an enzyme reactor tube located between the injector and detector. The present system should be applicable to a simple method for determining biologically important substances with high sensitivity.
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