MOLECULAR WIRING TYPE DNA SENSOR
| Project/Area Number |
11650851
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
工業物理化学
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| Research Institution | Soka University |
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Principal Investigator |
KUBO Izumi Soka University, Fac.Engineering, Prof., 工学部, 教授 (40214986)
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| Co-Investigator(Kenkyū-buntansha) |
SEKI Atsushi Soka University, Fac.Engineering, Asoc.Prof., 工学部, 助教授 (70226629)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | DNA Sensor / Intercalator / Molecular wire / GOD / FDH / Co(phen)_3 / Fe(phen)_3 / DNA sensor / Co(phen)_3 / Fe(phen)_3 / DNAセンサー / インターカレータ / 金電極 / フェナントロリンコバルト / フルクトースデヒドロゲナーゼ |
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| Research Abstract |
Electrochemical detection of specific DNA sequence of human papiroma virus was investigated using the gold electrode immobilized with probe DNA through a thiol group and tris(1,10-phenanthroline) cobalt (II) complex [Co(phen)_3^<2+>] as a hybridization indicator.
25-mer probe DNA modified with a thiol group at 5' end was immobilized on a gold electrode. Tris(1,10-phenanthroline) cobalt (II) chloride was prepared according to previously reported procedure. The gold electrode immobilized with probe DNA was immersed into buffer solution (pH 7.0) containing target DNA.Hybridization reaction was performed for an hour at 65 C。.After the hybridization, the electrode was immersed into Co(phen)_3^<2+>/0.1M phosphate buffer (pH 7.0). Cyclic voltammetry was performed in Co(phen)_3^<2+>/0.1 M phosphate buffer (pH 7.0).
Anodic peak current of cyclic voltammograms of 0.1 mM Co(phen)_3^<2+>/0.1 M phosphate buffer (pH 7.0) using the probe DNA-modified gold electrode hybridized with target DNA was larger
… More
than that using the probe DNA-modified gold electrode.
When the concentration of Co(phen)_3^<2+> was 0.1 mM, the response currents depended on target DNA concentration. However, at another Co(phen)_3^<2+> concentrations of 0.2 mM, 0.3mM and 1.0 mM examined, the response currents did not clearly depend on target DNA concentration.
The response currents were linearly related to target DNA concentration in the range from 10^<-7> M to 10^<-4> M with a slope of 10.5 nA / decade. Similar measurement was performed using the probe DNA-modified gold electrode reacted with 25-mer poly-deoxy A as non-complementary DNA.The response currents were hardly increased in the same range. On the other hand, all currents were slightly decreased from that using probe DNA-modified electrode. It seems that the surface of DNA-modified electrode suffer from chemical alteration because the electrode was repeatedly used increasing the concentration of target DNA (or poly-deoxy A) after one measurement. From these results, it was shown that a certain sequence of DNA, which is complementary to probe DNA, can be detected using cobalt phenanthroline complex and probe DNA-modified gold electrode. Less
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Research Products
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